Podcast
Questions and Answers
What is the purpose of gel electrophoresis?
What is the purpose of gel electrophoresis?
- To crystallize nucleic acids and proteins
- To combine nucleic acids and proteins
- To analyze the size and charge of nucleic acids and proteins (correct)
- To extract nucleic acids and proteins
Why is agarose used as a medium in gel electrophoresis?
Why is agarose used as a medium in gel electrophoresis?
- To form a network of bundles for molecule separation (correct)
- To inhibit the movement of molecules
- To accelerate the movement of molecules
- To neutralize the charge of molecules
How does the concentration of agarose affect pore size in the gel?
How does the concentration of agarose affect pore size in the gel?
- Higher concentration leads to smaller pores and denser matrix (correct)
- Higher concentration leads to larger pores and lighter matrix
- Higher concentration has no effect on pore size
- Higher concentration leads to no matrix formation
What role does the running buffer play in gel electrophoresis?
What role does the running buffer play in gel electrophoresis?
What determines the migration speed of molecules in an agarose gel?
What determines the migration speed of molecules in an agarose gel?
Why is it important to control the porosity of the gel in gel electrophoresis?
Why is it important to control the porosity of the gel in gel electrophoresis?
What is the purpose of adding a loading buffer to DNA samples in gel electrophoresis?
What is the purpose of adding a loading buffer to DNA samples in gel electrophoresis?
Why is it important to use the same batch of electrophoresis buffer in both the electrophoresis tank and the gel preparation?
Why is it important to use the same batch of electrophoresis buffer in both the electrophoresis tank and the gel preparation?
What is the function of a DNA ladder in gel electrophoresis?
What is the function of a DNA ladder in gel electrophoresis?
Why is it important to avoid puncturing the gel when loading DNA samples using a micropipette?
Why is it important to avoid puncturing the gel when loading DNA samples using a micropipette?
What is the purpose of adding ethidium bromide to the agarose gel solution?
What is the purpose of adding ethidium bromide to the agarose gel solution?
Why is it important to set the appropriate voltage in gel electrophoresis?
Why is it important to set the appropriate voltage in gel electrophoresis?
What does a loading buffer for DNA samples typically contain?
What does a loading buffer for DNA samples typically contain?
What happens when too much voltage is applied in gel electrophoresis?
What happens when too much voltage is applied in gel electrophoresis?
What is the role of Tris Borate EDTA (TBE) or Tris Acetate EDTA (TAE) buffers in DNA electrophoresis?
What is the role of Tris Borate EDTA (TBE) or Tris Acetate EDTA (TAE) buffers in DNA electrophoresis?
Which dye is commonly used to stain DNA by intercalating its strands, giving an orange color under UV light?
Which dye is commonly used to stain DNA by intercalating its strands, giving an orange color under UV light?
What is used as a standard to determine the size of unknown DNA molecules that were separated?
What is used as a standard to determine the size of unknown DNA molecules that were separated?
What is added to agarose gel solution to ensure that no air bubbles are formed during pouring?
What is added to agarose gel solution to ensure that no air bubbles are formed during pouring?
What is the purpose of agarose gel in gel electrophoresis?
What is the purpose of agarose gel in gel electrophoresis?
How does the concentration of agarose affect the size of pores in the gel?
How does the concentration of agarose affect the size of pores in the gel?
What is the function of a DNA ladder in gel electrophoresis?
What is the function of a DNA ladder in gel electrophoresis?
How does gel electrophoresis separate molecules that differ in size, charge or conformation?
How does gel electrophoresis separate molecules that differ in size, charge or conformation?
What is the role of Tris Borate EDTA (TBE) or Tris Acetate EDTA (TAE) buffers in DNA electrophoresis?
What is the role of Tris Borate EDTA (TBE) or Tris Acetate EDTA (TAE) buffers in DNA electrophoresis?
Why do large molecules migrate slower than small ones in agarose gel electrophoresis?
Why do large molecules migrate slower than small ones in agarose gel electrophoresis?
What determines the migration speed of molecules in an agarose gel during electrophoresis?
What determines the migration speed of molecules in an agarose gel during electrophoresis?
What is the purpose of adding ethidium bromide to the agarose gel solution?
What is the purpose of adding ethidium bromide to the agarose gel solution?
What is added to agarose gel solution to ensure that no air bubbles are formed during pouring?
What is added to agarose gel solution to ensure that no air bubbles are formed during pouring?
Why is it important to control the porosity of the gel in gel electrophoresis?
Why is it important to control the porosity of the gel in gel electrophoresis?
What role does the running buffer play in gel electrophoresis?
What role does the running buffer play in gel electrophoresis?
What is used as a standard to determine the size of unknown DNA molecules that were separated?
What is used as a standard to determine the size of unknown DNA molecules that were separated?
What is the purpose of adding a dye to the loading buffer in DNA electrophoresis?
What is the purpose of adding a dye to the loading buffer in DNA electrophoresis?
What is the function of the density agent added to the loading buffer in DNA electrophoresis?
What is the function of the density agent added to the loading buffer in DNA electrophoresis?
What is the purpose of the UV-Transilluminator in gel electrophoresis?
What is the purpose of the UV-Transilluminator in gel electrophoresis?
Why is GelRed considered a safer alternative to ethidium bromide in DNA electrophoresis?
Why is GelRed considered a safer alternative to ethidium bromide in DNA electrophoresis?
What is the role of a DNA ladder in gel electrophoresis?
What is the role of a DNA ladder in gel electrophoresis?
Why is it important to use the same batch of electrophoresis buffer in both the electrophoresis tank and the gel preparation?
Why is it important to use the same batch of electrophoresis buffer in both the electrophoresis tank and the gel preparation?
What determines the migration speed of molecules in an agarose gel during electrophoresis?
What determines the migration speed of molecules in an agarose gel during electrophoresis?
What does a loading buffer for DNA samples typically contain in gel electrophoresis?
What does a loading buffer for DNA samples typically contain in gel electrophoresis?
What happens when too much voltage is applied in gel electrophoresis?
What happens when too much voltage is applied in gel electrophoresis?
Why is it important to avoid puncturing the gel when loading DNA samples using a micropipette?
Why is it important to avoid puncturing the gel when loading DNA samples using a micropipette?
How does the concentration of agarose affect pore size in the gel during electrophoresis?
How does the concentration of agarose affect pore size in the gel during electrophoresis?
Why is it important to set the appropriate voltage in gel electrophoresis?
Why is it important to set the appropriate voltage in gel electrophoresis?
