Podcast
Questions and Answers
What is the process of introducing foreign DNA into a bacterial cell called?
What is the process of introducing foreign DNA into a bacterial cell called?
What is the enzyme used to catalyze the process of joining two pieces of DNA from different sources together?
What is the enzyme used to catalyze the process of joining two pieces of DNA from different sources together?
What are proteins that cleave DNA molecules at specific sites called?
What are proteins that cleave DNA molecules at specific sites called?
Which conformation of plasmid DNA migrates fastest in a gel during electrophoresis?
Which conformation of plasmid DNA migrates fastest in a gel during electrophoresis?
Signup and view all the answers
What is the first step in rapidly isolating plasmid?
What is the first step in rapidly isolating plasmid?
Signup and view all the answers
What is the purpose of using molecular weight markers during electrophoresis?
What is the purpose of using molecular weight markers during electrophoresis?
Signup and view all the answers
What is the process of cutting plasmid vector with AvaI called?
What is the process of cutting plasmid vector with AvaI called?
Signup and view all the answers
What is the main function of restriction enzymes in bacterial cells?
What is the main function of restriction enzymes in bacterial cells?
Signup and view all the answers
What is the primary purpose of cloning a piece of DNA using endonucleases?
What is the primary purpose of cloning a piece of DNA using endonucleases?
Signup and view all the answers
What is required for DNA ligation to occur?
What is required for DNA ligation to occur?
Signup and view all the answers
How can expression of a cloned gene be accomplished?
How can expression of a cloned gene be accomplished?
Signup and view all the answers
What type of gel electrophoresis has high resolving power and separates DNA only over a narrow size range?
What type of gel electrophoresis has high resolving power and separates DNA only over a narrow size range?
Signup and view all the answers
Which type of molecules can be resolved from one another with the applied electric field in polyacrylamide gel electrophoresis?
Which type of molecules can be resolved from one another with the applied electric field in polyacrylamide gel electrophoresis?
Signup and view all the answers
What can plasmids carry naturally?
What can plasmids carry naturally?
Signup and view all the answers
What can plasmids be engineered to be useful for?
What can plasmids be engineered to be useful for?
Signup and view all the answers
What is the cloning marker for plasmid vectors that contain the lacZ gene?
What is the cloning marker for plasmid vectors that contain the lacZ gene?
Signup and view all the answers
Which type of gel electrophoresis has less resolving power and separates DNA molecules of up to tens, and even hundreds, of kilobases?
Which type of gel electrophoresis has less resolving power and separates DNA molecules of up to tens, and even hundreds, of kilobases?
Signup and view all the answers
Study Notes
Genetic Engineering
- The process of introducing foreign DNA into a bacterial cell is called transformation.
DNA Manipulation
- Ligase is the enzyme used to catalyze the process of joining two pieces of DNA from different sources together.
- Restriction enzymes are proteins that cleave DNA molecules at specific sites.
- The main function of restriction enzymes in bacterial cells is to protect against viral infections.
Plasmid DNA
- The supercoiled conformation of plasmid DNA migrates fastest in a gel during electrophoresis.
- The first step in rapidly isolating plasmid is alkaline lysis.
- Cutting plasmid vector with AvaI is called restriction.
Electrophoresis
- The purpose of using molecular weight markers during electrophoresis is to determine the size of the DNA fragments.
- Polyacrylamide gel electrophoresis (PAGE) has high resolving power and separates DNA only over a narrow size range.
- Agarose gel electrophoresis has less resolving power and separates DNA molecules of up to tens, and even hundreds, of kilobases.
- In polyacrylamide gel electrophoresis, small molecules such as oligonucleotides can be resolved from one another with the applied electric field.
Cloning
- The primary purpose of cloning a piece of DNA using endonucleases is to amplify the DNA fragment.
- DNA ligation requires ATP and a cofactor such as NAD+ or ATP.
- Expression of a cloned gene can be accomplished by inducing the transcription and translation of the gene in a host cell.
Plasmid Vectors
- Plasmids can carry antibiotic resistance genes naturally.
- Plasmids can be engineered to be useful for protein expression, gene therapy, and vaccine development.
- The cloning marker for plasmid vectors that contain the lacZ gene is β-galactosidase.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Related Documents
Description
Test your knowledge about the process of separating DNA and RNA molecules through gel electrophoresis. This quiz covers topics such as gel matrix, resolving power, and staining methods for visualizing bands.
