mRNA Albumin Probe and In Situ Hybridization Quiz
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Questions and Answers

What is the purpose of in situ hybridization in the medical laboratory?

  • Identify iron-protein complexes in cells
  • Localize radioactive material within tissues
  • Analyze lipid content in tissues
  • Detect gene sequences or mRNAs of cells (correct)

How does autoradiography contribute to the detection of substances in tissues?

  • Identifies iron-protein complexes in cytoplasm
  • Measures hemoglobin levels in tissues
  • Uses photographic emulsion to localize radioactive material (correct)
  • Analyzes lipid content of normoblasts

What is the major storage form of iron in cells?

  • Normoblasts
  • Iron-protein complexes
  • Hemosiderin (correct)
  • Histocytes

Which reaction is used to detect polysaccharides like glycogen?

<p>PAS-amylase reaction (D)</p> Signup and view all the answers

What type of substances are detected using Perls' Prussian blue reaction?

<p>Iron (C)</p> Signup and view all the answers

What is the technique commonly used to study virus-infected cells and cancer cells with unique gene expression in tumors microscopically?

<p>ISH (C)</p> Signup and view all the answers

Which histochemical procedure is used for the detection of polysaccharides like glycogen in tissues?

<p>PAS-amylase reaction (B)</p> Signup and view all the answers

What is the purpose of Perls' Prussian blue reaction in a histological study?

<p>Detecting iron (C)</p> Signup and view all the answers

In which medical laboratory technique would one commonly employ ISH to detect specific gene expressions?

<p>In Situ Hybridization (ISH) (A)</p> Signup and view all the answers

Which of the following is NOT a reaction commonly used for the detection of biomolecules in histological studies?

<p>Perls' Prussian blue reaction for iron (A)</p> Signup and view all the answers

Which of the following is NOT a use of In Situ Hybridization (ISH) as described in the text?

<p>Detecting the presence of a specific protein in cells (C)</p> Signup and view all the answers

What is the main purpose of using mRNA Albumin Probe in In Situ Hybridization (ISH)?

<p>To identify specific messenger RNAs in cells (D)</p> Signup and view all the answers

Which enzyme histochemical procedure would be most suitable for detecting polysaccharides in tissues?

<p>PAS-amylase reaction (B)</p> Signup and view all the answers

What is one key advantage of using In Situ Hybridization (ISH) for identifying messenger RNAs compared to other techniques?

<p>ISH allows visualization of RNA sequences in their cellular context (A)</p> Signup and view all the answers

In which scenario would Perls' Prussian blue reaction be most useful in a medical laboratory setting?

<p>To determine iron accumulation in tissues (D)</p> Signup and view all the answers

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