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Northern Blot Technique in Molecular Biology

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Northern Blot Technique in Molecular Biology

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Questions and Answers

What is the main purpose of the northern blot technique in molecular biology research?

To study gene expression by detecting RNA in a sample (correct)

To study gene structure by detecting DNA in a sample

To study cellular metabolism by detecting DNA in a sample

To study protein expression by detecting mRNA in a sample

Who developed the northern blot technique?

Watson and Crick

Francis Crick

James Alwine, David Kemp, and George Stark (correct)

Edwin Southern

What is the major difference between the northern blot and the Southern blot techniques?

The northern blot was developed at Stanford University, while the Southern blot was developed at Yale University

The northern blot uses capillary transfer, while the Southern blot uses gel electrophoresis

There is no major difference between the two techniques

The northern blot analyzes RNA, while the Southern blot analyzes DNA (correct)

Which chromatography technique can be used to isolate eukaryotic mRNA for the northern blot?

<p>Oligo (dT) cellulose chromatography</p> Signup and view all the answers

What is the primary detection method used in northern blotting?

<p>Hybridization probe complementary to part of or the entire target sequence</p> Signup and view all the answers

Study Notes

Northern Blot Technique

The primary purpose of the northern blot technique is to detect and quantify specific RNA molecules in a sample.
The northern blot technique was developed by James E. Alwine, David J. Kemp, and George R. Stark in 1977.

Comparison with Southern Blot

The main difference between the northern blot and Southern blot techniques is the type of nucleic acid analyzed: the northern blot detects RNA, while the Southern blot detects DNA.

RNA Isolation

Size-exclusion chromatography, also known as gel filtration chromatography, can be used to isolate eukaryotic mRNA for the northern blot.

Detection Method

The primary detection method used in northern blotting is autoradiography or chemiluminescence, which involves hybridizing a labeled probe to the target RNA molecule and then detecting the labeled probe.

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Description

Test your knowledge on the northern blot technique which is used to study gene expression by detecting RNA in a sample. Explore how it is used to observe gene expression rates and cellular control over structure and function.