Podcast
Questions and Answers
Which of the following is NOT a method of non-thermal processing?
Which of the following is NOT a method of non-thermal processing?
- High Hydrostatic Pressure (HHP)
- Thermal processing (correct)
- Ultrasound (US)
- Pulsed Electric Field (PEF)
High Pressure Processing (HPP) can degrade vitamins, flavor, and color molecules.
High Pressure Processing (HPP) can degrade vitamins, flavor, and color molecules.
False (B)
What is the main purpose of using Pulsed Electric Field (PEF) in food processing?
What is the main purpose of using Pulsed Electric Field (PEF) in food processing?
To inactivate pathogenic microorganisms
High Hydrostatic Pressure (HHP) can apply pressures up to _____ MPa.
High Hydrostatic Pressure (HHP) can apply pressures up to _____ MPa.
Match the preservation technique with its benefit:
Match the preservation technique with its benefit:
What is the typical pressure level used in High Pressure Processing to achieve a tenfold reduction in bacterial cells?
What is the typical pressure level used in High Pressure Processing to achieve a tenfold reduction in bacterial cells?
Bacterial spores are easily destroyed by High Pressure Processing.
Bacterial spores are easily destroyed by High Pressure Processing.
What is the shelf life of fruit-based chilled HPP products?
What is the shelf life of fruit-based chilled HPP products?
What is the typical electric field intensity used in PEF treatment?
What is the typical electric field intensity used in PEF treatment?
PEF treatment can be applied to any type of food product without restrictions.
PEF treatment can be applied to any type of food product without restrictions.
What is the mechanism called that allows cell membranes to develop pores during PEF treatment?
What is the mechanism called that allows cell membranes to develop pores during PEF treatment?
The shelf life of raw skim milk treated with PEF was increased to _____ days.
The shelf life of raw skim milk treated with PEF was increased to _____ days.
Match the food item with its corresponding PEF treatment parameters:
Match the food item with its corresponding PEF treatment parameters:
What was the shelf life increase of apple juice after PEF treatment compared to fresh squeezed juice?
What was the shelf life increase of apple juice after PEF treatment compared to fresh squeezed juice?
PEF treatment causes significant physical or chemical changes in ascorbic acid or sugars.
PEF treatment causes significant physical or chemical changes in ascorbic acid or sugars.
What is the typical duration of the pulses used in PEF treatment?
What is the typical duration of the pulses used in PEF treatment?
What is a major disadvantage of using ultrasound for sterilization?
What is a major disadvantage of using ultrasound for sterilization?
The oscillating magnetic field (OMF) can have a static intensity over time.
The oscillating magnetic field (OMF) can have a static intensity over time.
What are free radicals generated during the collapse of bubbles responsible for in the sterilization process?
What are free radicals generated during the collapse of bubbles responsible for in the sterilization process?
OMF treatment for food preservation typically involves a frequency range of _____ kHz.
OMF treatment for food preservation typically involves a frequency range of _____ kHz.
Match the following terms with their definitions:
Match the following terms with their definitions:
What is the primary benefit of treating raw skim milk with 80°C for 6 seconds followed by PEF treatment?
What is the primary benefit of treating raw skim milk with 80°C for 6 seconds followed by PEF treatment?
Electroporation refers to the alteration of membrane permeability in microorganisms.
Electroporation refers to the alteration of membrane permeability in microorganisms.
List two mechanisms through which pulse light disrupts bacterial cells.
List two mechanisms through which pulse light disrupts bacterial cells.
Ultrasound waves are defined as energy generated by sound waves of over ______ vibrations per second.
Ultrasound waves are defined as energy generated by sound waves of over ______ vibrations per second.
Match the following types of treatment with their main characteristic:
Match the following types of treatment with their main characteristic:
Which component is NOT a part of the pulse light treatment process?
Which component is NOT a part of the pulse light treatment process?
The effectiveness of pulse light treatment does not depend on the intensity of light delivered.
The effectiveness of pulse light treatment does not depend on the intensity of light delivered.
What is a major limitation of pulse light treatment?
What is a major limitation of pulse light treatment?
Flashcards
High Pressure Processing (HPP)
High Pressure Processing (HPP)
A non-thermal food preservation method using high pressure to kill microorganisms without altering taste, color or nutrients.
MegaPascal (MPa)
MegaPascal (MPa)
Measure of pressure applied in HPP, typically reaching up to 900MPa.
Isostatic pressure
Isostatic pressure
The ability of HPP to evenly distribute pressure throughout the food package.
Holding Time
Holding Time
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Pulsed Electric Field (PEF)
Pulsed Electric Field (PEF)
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PEF's primary purpose
PEF's primary purpose
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PEF's advantage
PEF's advantage
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PEF's application
PEF's application
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Electroporation
Electroporation
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Field Strength (kV/cm)
Field Strength (kV/cm)
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Pulse Duration
Pulse Duration
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Frequency
Frequency
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PEF Treatment Chamber
PEF Treatment Chamber
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PEF Treatment of Apple Juice
PEF Treatment of Apple Juice
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PEF Treatment of Milk
PEF Treatment of Milk
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How does ultrasound kill microorganisms?
How does ultrasound kill microorganisms?
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What types of food are best suited for ultrasound sterilization?
What types of food are best suited for ultrasound sterilization?
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What is Oscillating Magnetic Field (OMF)?
What is Oscillating Magnetic Field (OMF)?
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How does OMF affect microorganisms?
How does OMF affect microorganisms?
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Explain the OMF treatment process.
Explain the OMF treatment process.
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Pulse Light
Pulse Light
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Ultrasound Waves
Ultrasound Waves
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High Voltage Feed
High Voltage Feed
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Photochemical Mechanism
Photochemical Mechanism
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Photothermal Mechanism
Photothermal Mechanism
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Cavitation
Cavitation
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Packaging Material Transparency
Packaging Material Transparency
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Study Notes
Other Preservation Techniques
- Non-thermal processing is a promising approach for fruit juice and beverage preservation.
- Products using non-thermal techniques retain more sensory and nutritional qualities compared to traditionally processed products.
- Examples include high hydrostatic pressure (HHP), pulsed electric field (PEF), and ultrasound (US).
High Pressure Processing (HPP)
- Also known as high hydrostatic pressure (HHP)
- HHP uses up to 900 MPa to kill microorganisms in food, without degrading vitamins, flavour, or colour.
- High pressures (up to 1000 MPa) are applied to submerged food packages, distributing pressure evenly.
- 350 MPa for 30 minutes or 400 MPa for 5 minutes significantly reduces vegetative cells of bacteria, yeasts, or molds.
- HPP doesn't involve heating, so sensory and nutritional qualities are preserved similar to unprocessed products.
- Processing times are faster than thermal processing.
- Bacterial spores are resistant; therefore, products are chilled or refrigerated.
- Shelf-life varies by product type (e.g., fruit-based chilled products have a shelf-life of 21-28 days, while meat products have a shelf-life of 30-60 days). Meat products may use curing agents to prevent botulism.
Pulse Electric Field (PEF)
- Non-thermal pasteurization technique.
- Used on liquid foods (liquid egg, milk, fruit juices).
- Main purpose is to inactivate pathogenic microorganisms.
- Minimizes energy loss associated with heating foods.
- Superior to traditional heat treatment, as it avoids or greatly reduces detrimental changes in the sensor and physical properties of foods.
- Involves applying pulses of high voltage (typically between 20-80 kV/cm) to food located between electrodes.
- Pulses have specific frequency, duration, and intensity.
- Application is restricted to foods that can withstand high electric fields. PEF treatment chamber consists of at least two electrodes and insulation, creating a volume where food receives pulses. Electrodes are made of inert materials (e.g., titanium).
- The application of electric fields to biological cells causes a buildup of electrical charges at the cell membrane. The resulting pore formation increases membrane permeability leading to cell content loss or intrusion of external media.
- Treatment effectiveness depends on the intensity and number of pulses (frequency).
- Apple juice treated with PEF had a longer shelf life (28 days) compared to fresh squeezed apple juice (21 days).
- No significant changes in ascorbic acid or sugars were observed.
Pulse Light
- Non-thermal method.
- Three main components: power supply, pulse configuration device, and xenon lamp.
- Converts high-speed electronic pulses into high-energy light pulses.
- Uses high-intensity flashes of broad-spectrum light (UV to infrared) at short durations.
- High energy is delivered to the lamp, producing intense pulses of light focused on the treatment area.
- Exposing food to this light disrupts bacterial cell membranes.
- Effectiveness depends on intensity and number of pulses.
Ultrasound Waves
- Defined as energy generated by sound waves over 20,000 vibrations per second.
- Sonic waves with frequencies above human hearing (16-20 kHz).
- Generated by mechanical vibrations above 18 kHz.
- During implosion, very high temperatures and pressures are reached inside bubbles.
- Potentially most useful for sterilizing liquids (e.g., milk and juices).
- Disadvantages have limited lethal effect and effective if applied with pressure (e.g., Mano Sonication or Mano Thermo Sonication).
Oscillating Magnetic Field (OMF)
- Static Magnetic Field (SMF): Constant magnetic field intensity.
- Food is treated with magnetic pulses (millisecond range) with exposure times of 25 to over 100ms
- Frequency applied is between 5-50 kHz and field strength is between 5-50 Tesla.
- Effectiveness is dependent on pulse number and frequency.
- OMF might loosen bonds between ions and proteins in microorganisms.
- The effect on microorganisms may be due to the rotating electric field formed by the variable magnetic field.
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