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Questions and Answers
The NOM-062-ZOO-1999 establishes technical specifications for the production, care, and use of laboratory animals.
The NOM-062-ZOO-1999 establishes technical specifications for the production, care, and use of laboratory animals.
True (A)
NOM-062-ZOO-1999 applies exclusively to government-funded research facilities throughout Mexico.
NOM-062-ZOO-1999 applies exclusively to government-funded research facilities throughout Mexico.
False (B)
According to NOM-062-ZOO-1999, only type A facilities are required to have an internal committee for the care and use of laboratory animals.
According to NOM-062-ZOO-1999, only type A facilities are required to have an internal committee for the care and use of laboratory animals.
False (B)
The NOM-062-ZOO-1999 regulation is solely focused on animal welfare and does not address the reliability of scientific results.
The NOM-062-ZOO-1999 regulation is solely focused on animal welfare and does not address the reliability of scientific results.
Rodents, rabbits, carnivores, non-human primates, and pigs are examples of species covered under the NOM-062-ZOO-1999.
Rodents, rabbits, carnivores, non-human primates, and pigs are examples of species covered under the NOM-062-ZOO-1999.
According to the guidelines, the inadequate treatment of laboratory animals decreases stress factors, thereby improving their resistance to diseases.
According to the guidelines, the inadequate treatment of laboratory animals decreases stress factors, thereby improving their resistance to diseases.
One of the key objectives is minimizing animal use while maintaining reliable results.
One of the key objectives is minimizing animal use while maintaining reliable results.
It is unnecessary for facilities to have a designated responsible person for operations involving laboratory animals.
It is unnecessary for facilities to have a designated responsible person for operations involving laboratory animals.
Technical staff working with lab animals are not required to meet any specific qualifications, as long as they have experience.
Technical staff working with lab animals are not required to meet any specific qualifications, as long as they have experience.
According to NOM-062-ZOO-1999, facilities are discouraged from designating a veterinarian and an administrative manager to oversee compliance with the Standard.
According to NOM-062-ZOO-1999, facilities are discouraged from designating a veterinarian and an administrative manager to oversee compliance with the Standard.
Health certificates only need to include the supplier information.
Health certificates only need to include the supplier information.
Health and quality certificates are not requirements for research animals.
Health and quality certificates are not requirements for research animals.
Proper record-keeping is not necessary to maintain research validity.
Proper record-keeping is not necessary to maintain research validity.
Laboratory animal diet must be free of additives, drugs, hormones, antibiotics, pesticides and contaminants.
Laboratory animal diet must be free of additives, drugs, hormones, antibiotics, pesticides and contaminants.
Rodents in the laboratory must be maintained without a cage.
Rodents in the laboratory must be maintained without a cage.
Environmental controls, such as temperature, humidity, and lighting, are unnecessary requirements.
Environmental controls, such as temperature, humidity, and lighting, are unnecessary requirements.
All the factors considered with laboratory animals must be verified regularly.
All the factors considered with laboratory animals must be verified regularly.
Specifications for primary enclosures are recommended during transport.
Specifications for primary enclosures are recommended during transport.
A key consideration when administering fluids and substances to lab animals is minimizing distress and pain.
A key consideration when administering fluids and substances to lab animals is minimizing distress and pain.
There is no need to provide proper pain management to animals post administering fluids and substances.
There is no need to provide proper pain management to animals post administering fluids and substances.
According to the information, electrocution is considered an acceptable method of euthanasia for dogs.
According to the information, electrocution is considered an acceptable method of euthanasia for dogs.
A veterinarian's expertise is irrelevant when dealing with exotic or rare species in research settings.
A veterinarian's expertise is irrelevant when dealing with exotic or rare species in research settings.
According to the guideline, it's acceptable to refrigerate bodies before allowing volatilization.
According to the guideline, it's acceptable to refrigerate bodies before allowing volatilization.
It is always ethically sound to prioritize research needs over animal welfare during euthanasia.
It is always ethically sound to prioritize research needs over animal welfare during euthanasia.
One benefit of group housing for primates in research settings is that it addresses their social needs.
One benefit of group housing for primates in research settings is that it addresses their social needs.
According to the information, there is no minimum air change per hour requirement.
According to the information, there is no minimum air change per hour requirement.
The species doesn't matter when it comes to climate control.
The species doesn't matter when it comes to climate control.
According to the information, colony housing does not require speacial precautions.
According to the information, colony housing does not require speacial precautions.
It is unnecessary to use trained personnel when dealing with laboratory primates.
It is unnecessary to use trained personnel when dealing with laboratory primates.
According to the information, it is unnecessary to isolate sick animals.
According to the information, it is unnecessary to isolate sick animals.
Medical evaluations and preventive measures are not part of biosafety and occupational health protocols.
Medical evaluations and preventive measures are not part of biosafety and occupational health protocols.
All euthanasia methods work for all species and situations.
All euthanasia methods work for all species and situations.
The ultimate goal of euthanasia in research animals is to induce death in a inhumane way.
The ultimate goal of euthanasia in research animals is to induce death in a inhumane way.
Rapid unconsciousness is not really a criteria for properly performing euthanasia.
Rapid unconsciousness is not really a criteria for properly performing euthanasia.
According to the document, it is unethical to check if death has occured.
According to the document, it is unethical to check if death has occured.
Under all circumstances, it is ethical to dispose of biological products and lab experiments remains together in the same container.
Under all circumstances, it is ethical to dispose of biological products and lab experiments remains together in the same container.
The selection of appropriate euthanasia methods isn't important to ethical lab procedures.
The selection of appropriate euthanasia methods isn't important to ethical lab procedures.
It's unnecessary for personnel administering euthanasia to have all types of training.
It's unnecessary for personnel administering euthanasia to have all types of training.
The lab conditions need to be thoroughly sanitary to assure there are no contaminants.
The lab conditions need to be thoroughly sanitary to assure there are no contaminants.
With euthanasia of dogs and cats, there is no need to handle the animal with care.
With euthanasia of dogs and cats, there is no need to handle the animal with care.
Flashcards
NOM-062-ZOO-1999
NOM-062-ZOO-1999
Mexican regulation for laboratory animal care and use.
NOM-062-ZOO-1999 aims
NOM-062-ZOO-1999 aims
Ensures animal welfare and reliable scientific results are achieved.
Scope of NOM-062-ZOO-1999
Scope of NOM-062-ZOO-1999
Mandatory throughout Mexico for facilities handling specific laboratory animals.
Common lab animals
Common lab animals
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Reliable results
Reliable results
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Key objective
Key objective
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Uniform Criteria
Uniform Criteria
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Ensure Animal Welfare
Ensure Animal Welfare
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Minimize animal use
Minimize animal use
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Facility Requirements
Facility Requirements
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Staff Qualifications
Staff Qualifications
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Employee training
Employee training
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Vivariums
Vivariums
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Animal Committee
Animal Committee
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Animal Sourcing
Animal Sourcing
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Health Certificate
Health Certificate
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Identification
Identification
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Health certificate
Health certificate
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Optimal diet
Optimal diet
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Proper Storage
Proper Storage
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indoor/outdoor facilities
indoor/outdoor facilities
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Species guidelines
Species guidelines
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Transit needs
Transit needs
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Experimental Techniques
Experimental Techniques
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Euthanasia Protocols
Euthanasia Protocols
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Euthanasia
Euthanasia
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Euthanasia: key goals
Euthanasia: key goals
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Euthanasia rules
Euthanasia rules
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Selection criteria
Selection criteria
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Understanding Mechanisms
Understanding Mechanisms
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Competence
Competence
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inhalant agents
inhalant agents
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Equipment Maintenance
Equipment Maintenance
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Cats and Dogs approach
Cats and Dogs approach
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Verify cessation
Verify cessation
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Responsibilities
Responsibilities
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Primate Housing
Primate Housing
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Group housing
Group housing
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Proper Lightning
Proper Lightning
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Appropriate Features
Appropriate Features
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Study Notes
- NOM-062-ZOO-1999 covers animal research ethics and regulations
Introduction to NOM-062-ZOO-1999
- Official Mexican regulation addresses the care and use of laboratory animals
- Establishes technical guidelines for the production, care, and use of laboratory animals
- Focuses on animal welfare and ensuring reliable scientific results
Scope and Applicability
- Mandatory throughout Mexico
- Applies to places that handle specific laboratory animals
- Specific laboratory animals: rodents (rats, mice, guinea pigs, hamsters, gerbils), rabbits, carnivores (dogs, cats), non-human primates, and pigs
Considerations
- Achieving reliable research results requires optimal conditions for lab animals
- It is important to use the least amount of animals as possible
- Poor treatment and handling during movement increases stress and disease susceptibility
Key Objectives
- Promote proper production, care, and handling of lab animals
- Establish consistent standards for animal care and handling
- Ensure animal welfare in scientific research, technological advancement, and education
- Minimize animal usage while maintaining reliable results
Personnel Requirements
- Facilities require one assigned responsible person for operations
- Technical staff need to meet specific qualifications
- Proper training in animal care, handling, and ethical factors is needed
- All vivariums need to assign a vet and administrative manager for compliance
- Type B and C facilities required to have an "Internal Committee for the Care and Use of Laboratory Animals"
Animal Sourcing and Documentation
- Guidelines that need to be followed for obtaining research animals
- Health and quality certificates are required
- Proper identification and record-keeping are a requirement as well
Health certification
- All animals need documents that confirm their health and quality, including:
- Supplier information
- Specie
- Race, strain
- Total number of animals & Sex
- Birth date
- Lab tests and dates
- Vet signature
Facility Requirements
- Specifications are provided for indoor and outdoor housing
- There are Guidelines for housing of different species
- Environmental controls (temperature, humidity, lighting) are a requirement
Transportation Guidelines
- Proper animal consignment procedures need to be followed
- There are specific requirements for primary enclosures during transport
- Requires appropriate vehicles for animal
- There are provisions concerning food, water, and care during transit
Experimental Techniques
- Focuses on reducing animal distress and pain
- Procedures are provided for administering fluids and substances
Euthanasia Protocols
- Looks at objectives and general considerations for euthanasia
- Gives recommended and conditionally acceptable methods in carrying out euthanasia
- Lists of prohibited practices and methods
Biosafety and Occupational Health
- General safety standards for personnel
- Required training and protective equipment
- Measures for medical evaluations and prevention
- Proper disposal of biological products and animal remains
Introduction to Animal Euthanasia
- Humane method to end a research animal's life
- Primary objectives are to reduce pain, stress, and suffering
- Must cause a rapid loss of consciousness and death
- Factors: Animal welfare, research needs, and personnel safety
General Considerations
- Must comply with federal and state laws on drug use
- No single method works for all species/scenarios
- Selection criteria: Research compatibility, speed, reliability, safety
- Responsibility lies with the researcher/veterinarian
- Ethics committee approval is needed for non-standard methods
Criteria for Choosing Euthanasia Methods
- Induce death without panic or anxiety
- Rapid drop in consciousness
- Should be able to repeat the method and get similar results
- Safe for personnel
- Compatible with research goals
- Minimal environmental effects
- Fail-safe
- Should be done in separate location from animal housing
Personnel Requirements (Euthanasia)
- Must have proper training and related experience
- Knows normal animal behavior
- Understands the euthanasia mechanism
- Proves skill under supervision
Inhalant Agents
- Provide quick exposure to high concentrations
- Is reliant on well-maintained equipment
- Requires safety precautions
- Is less effective on Neonates given their resistance to hypoxemia
- Requires clean chambers that are free of odors
- Only same species together in appropriate numbers
Recommended Inhalant Anesthetics
- For animals that are 7 kg or less or that are difficult to perform venipuncture
- Recommended Order: halothane, enflurane, isoflurane, methoxyflurane, ether
- Can be combined with nitrous oxide
- Use closed container with anesthetic-soaked cotton
- Ensure sufficient oxygen to counter possible hypoxemia
- Anesthetic should not come in contact with skin
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