Podcast
Questions and Answers
What is the primary process that the nitrite test relies on?
What is the primary process that the nitrite test relies on?
Why is a first morning urine specimen preferred for the nitrite test?
Why is a first morning urine specimen preferred for the nitrite test?
What indicates a positive result in a nitrite test?
What indicates a positive result in a nitrite test?
What can lead to a false positive result in the nitrite test?
What can lead to a false positive result in the nitrite test?
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Which reagent strip contains p-arsanilic acid for nitrite testing?
Which reagent strip contains p-arsanilic acid for nitrite testing?
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What is the detection threshold for nitrite in the Multistix test?
What is the detection threshold for nitrite in the Multistix test?
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How long does the Chemstrip test take to produce a result?
How long does the Chemstrip test take to produce a result?
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What should be interpreted as a negative result in the nitrite test?
What should be interpreted as a negative result in the nitrite test?
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What does the Chemstrip contain to react with nitrite?
What does the Chemstrip contain to react with nitrite?
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What is the minimum duration required for bladder incubation for effective nitrite testing?
What is the minimum duration required for bladder incubation for effective nitrite testing?
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Study Notes
Nitrite Testing in Urine
- Mechanism: The test relies on bacteria converting nitrate to nitrite in urine.
- Specimen Collection: A first morning specimen is ideal, as overnight bladder incubation (at least 4 hours) is needed for bacterial conversion.
- Positive Result: A positive result suggests a urinary tract infection (UTI) and warrants further culture testing. However, contamination after collection can also yield a positive result.
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Reagent Strips:
- Multistix: Uses p-arsanilic acid, which reacts with nitrite to create a diazonium salt, then couples with benzoquinoline to form a pink azo dye. Detects 0.075 mg/dL nitrite at 40 seconds.
- Chemstrip: Contains benzoquinoline and sulfanilamide, which react with nitrite to form a pink azo dye at 30 seconds. Detects 0.05 mg/dL nitrite.
- Interpretation Note: Pink spots or edges on the reagent strips are considered negative results.
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Description
This quiz covers the mechanisms and procedures for nitrite testing in urine, highlighting its significance in diagnosing urinary tract infections (UTIs). Learn about specimen collection, the use of reagent strips like Multistix and Chemstrip, and how to interpret the results accurately.
