Questions and Answers
Which sequencing technique is commonly referred to as NGS?
Illumina sequencing
What is one major difference between NGS and Sanger sequencing?
NGS can sequence both strands simultaneously, while Sanger sequencing cannot
Which sequencing technique uses the principle of dideoxy termination?
Sanger sequencing
What is the main advantage of NGS over Sanger sequencing?
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What is the goal of NGS?
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Study Notes
Next-Generation Sequencing (NGS)
- Next-Generation Sequencing (NGS) is commonly referred to as NGS.
- One major difference between NGS and Sanger sequencing is that NGS is high-throughput, parallel sequencing, whereas Sanger sequencing is low-throughput and serial.
Sanger Sequencing
- Sanger sequencing uses the principle of dideoxy termination.
- The main advantage of NGS over Sanger sequencing is its high-throughput capability and ability to analyze millions of DNA sequences in parallel, making it faster and more cost-effective.
Goals of NGS
- The goal of NGS is to determine the order of nucleotides (A, C, G, and T) that make up an organism's DNA or RNA.
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