Next Generation Sequencing (NGS) Overview

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Questions and Answers

What is the main difference between Next Generation Sequencing (NGS) and Sanger Sequencing?

  • NGS is faster than Sanger Sequencing
  • NGS is used for RNA sequencing, while Sanger Sequencing is used for DNA sequencing
  • NGS requires a reference genome, while Sanger Sequencing does not
  • NGS can sequence billions of DNA strands simultaneously, while Sanger Sequencing can only sequence one strand at a time (correct)

What was the duration of the Human Genome Project?

  • 13 years
  • 32 years (correct)
  • 10 years
  • 22 years

Why does Next Generation Sequencing (NGS) enable faster sequencing compared to the Human Genome Project?

  • It uses a different chemical process for sequencing
  • It requires less computational analysis
  • It can sequence billions of DNA strands simultaneously (correct)
  • It has better accuracy in sequencing

What enabled Next Generation Sequencing (NGS) to work effectively?

<p>The creation of a human reference DNA sequence by the Human Genome Project (B)</p>
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What is the key principle behind Next Generation Sequencing (NGS)?

<p>&quot;DNA can be cut into small pieces and sequenced, then assembled based on the reference genome&quot; (D)</p>
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