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DNA Sequencing Techniques

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Questions and Answers

What was the primary aim of the Human Genome Project?

To track inherited traits over generations

To produce maps showing the locations of genes

To identify genetic variations in different organisms

To determine the order of all the bases in the genome's DNA (correct)

What is the primary technique used in the first-generation sequencing of the Human Genome Project?

DNA/RNA sequencing

Sanger sequencing (correct)

Protein structure determination

Next Generation Sequencing

What is the main benefit of the Human Genome Project in relation to genetic testing?

Development of new gene editing techniques

Creation of many genetic tests (correct)

Advancement in stem cell research

Identification of all genetic variations

What is the purpose of sequencing techniques such as Sanger sequencing and Next Generation Sequencing?

To determine the primary structure of DNA, RNA, and proteins Signup and view all the answers

What is the main advantage of Illumina sequencing in NGS technology?

It can read 100 – 150 bp at a time Signup and view all the answers

Why is single-read sequencing best suited for small RNA sequencing?

Because it reads 100 – 150 bp at a time Signup and view all the answers

What is the purpose of paired-end (PE) sequencing in NGS technology?

To produce a higher number of SNV calls Signup and view all the answers

What is the significance of analyzing differential read-pair spacing in PE sequencing?

It allows removal of PCR duplicates Signup and view all the answers

What is the main purpose of high throughput sequencing (HTS) mentioned in the text?

To understand single-nucleotide polymorphisms Signup and view all the answers

When was Sanger sequencing first commercialized?

1986 Signup and view all the answers

What is the maximum length of DNA regions routinely sequenced using Sanger sequencing?

Up to 900 base pairs Signup and view all the answers

Which type of genetic variation does high throughput sequencing (HTS) help in understanding?

Single-nucleotide polymorphisms Signup and view all the answers