Mycobacteria and Acid-Fast Property
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Questions and Answers

What property of mycobacteria makes them acid-fast?

  • Their high lipid content in the cell wall (correct)
  • Their resistance to antibiotics
  • Their ability to survive in acidic environments
  • Their ability to form spores

What component of the cell wall is responsible for the acid-fast property of M. tuberculosis?

  • Nucleic acids
  • Carbohydrates
  • Mycolic acids (correct)
  • Proteins

What is the significance of the carbol fuchsin stain in identifying mycobacteria?

  • It helps to identify the shape of mycobacteria
  • It helps to determine the antibiotic resistance of mycobacteria
  • It helps to retain the stain despite subsequent treatment (correct)
  • It helps to differentiate between different species of mycobacteria

Study Notes

Mycobacteria Properties

  • Mycobacteria are acid-fast due to the high concentration of mycolic acids in their cell walls.
  • Mycolic acids are long-chain, alpha-alkyl, beta-hydroxy fatty acids that create a hydrophobic environment, making the cells resistant to acidic and alkaline conditions.
  • In M. tuberculosis, the cell wall component responsible for the acid-fast property is the unique mycolic acid-rich peptidoglycan layer.

Importance of Carbol Fuchsin Stain

  • The carbol fuchsin stain is a critical diagnostic tool for identifying mycobacteria, particularly M. tuberculosis.
  • This stain helps to differentiate mycobacteria from other bacteria due to their unique acid-fast properties.

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Description

Test your knowledge on the aerobic, acid-fast bacilli known as mycobacteria. Learn about their unique ability to retain the carbol fuchsin stain and the high lipid content of their cell wall that makes them acid-fast.

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