Podcast
Questions and Answers
What metal ions are crucial for creatine kinase activity?
What metal ions are crucial for creatine kinase activity?
Magnesium ions (Mg2+) are crucial for creatine kinase activity and form complexes with ATP and ADP, serving as obligate activating ions.
What is the role of creatine kinase in cellular energy metabolism?
What is the role of creatine kinase in cellular energy metabolism?
Creatine kinase plays a crucial role in cellular energy metabolism, particularly in muscle cells, by catalyzing the reversible phosphorylation of creatine by ATP.
What is the optimal pH value for the forward reaction of creatine kinase?
What is the optimal pH value for the forward reaction of creatine kinase?
The forward reaction of creatine kinase, which generates ATP from creatine phosphate, is favored at a pH of 9.0.
What metal ions inhibit creatine kinase activity?
What metal ions inhibit creatine kinase activity?
What is the molecular weight of creatine kinase?
What is the molecular weight of creatine kinase?
What is the role of creatine kinase during muscle contraction?
What is the role of creatine kinase during muscle contraction?
What compounds negatively affect CK activity?
What compounds negatively affect CK activity?
Why is maintaining low concentrations of chloride and sulfate ions essential in enzyme assay systems based on the reverse reaction?
Why is maintaining low concentrations of chloride and sulfate ions essential in enzyme assay systems based on the reverse reaction?
Which sulfhydryl compounds can help restore some of the lost activity of creatine kinase?
Which sulfhydryl compounds can help restore some of the lost activity of creatine kinase?
What is the advantage of using N-acetylcysteine in current assays?
What is the advantage of using N-acetylcysteine in current assays?
Which tissues contain the highest levels of CK activity?
Which tissues contain the highest levels of CK activity?
Which tissues exhibit significantly lower levels of CK activity compared to striated muscle and heart tissue?
Which tissues exhibit significantly lower levels of CK activity compared to striated muscle and heart tissue?
Which tissues are essentially devoid of CK activity?
Which tissues are essentially devoid of CK activity?
What are the molecular weights of the subunits composing creatine kinase?
What are the molecular weights of the subunits composing creatine kinase?
How many distinct pairs of subunits can combine to form various isoenzymes of creatine kinase?
How many distinct pairs of subunits can combine to form various isoenzymes of creatine kinase?
Based on electrophoretic mobility, how have the isoenzymes of creatine kinase been named?
Based on electrophoretic mobility, how have the isoenzymes of creatine kinase been named?
