Monosaccharides and Polysaccharides

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Questions and Answers

How does the orientation of the 1-OH group affect glucose?

  • It alters the number of covalent bonds within the glucose molecule.
  • It distinguishes glucose from other monosaccharides like fructose.
  • It determines whether glucose is a disaccharide or a polysaccharide.
  • It results in two isomers (α or β) of glucose. (correct)

What characteristic of monosaccharides facilitates their transportation between cells?

  • Their polar and hydrophilic nature. (correct)
  • Their non-polar molecular structure.
  • Their ability to form complex associations with proteins.
  • Their relatively large molecular weight.

Which process allows energy stored in the covalent bonds of monosaccharides to be released?

  • Hydrolysis.
  • Condensation.
  • Isomerization.
  • Oxidation via cellular respiration. (correct)

What dictates the properties of the polymer formed from monosaccharides?

<p>The monomers involved and their bonding arrangements. (A)</p> Signup and view all the answers

In what form do organisms typically store glucose for later use?

<p>As starch (in plants) or glycogen (in animals). (C)</p> Signup and view all the answers

What type of reaction creates a glycosidic bond?

<p>Condensation. (B)</p> Signup and view all the answers

If a researcher is studying the energy source that cells primarily utilize, which monosaccharide should they focus on?

<p>Glucose (A)</p> Signup and view all the answers

A scientist discovers a new disaccharide composed of two glucose molecules. What is this disaccharide most likely to be?

<p>Maltose. (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of monosaccharides?

<p>They readily dissolve in nonpolar substances. (B)</p> Signup and view all the answers

What kind of molecule is a glycoprotein?

<p>A complex of polysaccharide and a protein. (D)</p> Signup and view all the answers

How does the presence of double bonds in unsaturated fatty acids affect their physical properties, compared to saturated fatty acids?

<p>They decrease the melting point because the kinked structure reduces packing efficiency. (A)</p> Signup and view all the answers

Which statement best describes why saturated fats are typically solid at room temperature?

<p>The straight chains allow for tight packing and increased intermolecular forces. (C)</p> Signup and view all the answers

A scientist is analyzing a lipid sample and finds it is liquid at room temperature and contains multiple double bonds. What type of fatty acid is most likely present?

<p>Polyunsaturated fatty acid (A)</p> Signup and view all the answers

How does the molecular structure of unsaturated fatty acids affect the way they pack together, and what is the consequence of this packing?

<p>Kinked chains; lower melting point (D)</p> Signup and view all the answers

Which structural feature of saturated fatty acids allows them to have higher melting points compared to unsaturated fatty acids?

<p>Linear carbon chains (B)</p> Signup and view all the answers

A food manufacturer is deciding between using saturated or unsaturated fats in a product. They need a fat that remains solid at room temperature. Which type of fat should they choose and why?

<p>Saturated, because its straight chains allow for tighter packing. (D)</p> Signup and view all the answers

How do the intermolecular forces differ between saturated and unsaturated fatty acids, and how does this difference manifest in their physical state at room temperature?

<p>Saturated fats have stronger forces, resulting in a solid state. (B)</p> Signup and view all the answers

What is the primary structural difference between monounsaturated and polyunsaturated fatty acids, and how does this difference affect their properties?

<p>Number of double bonds; polyunsaturated acids have multiple, leading to lower melting points. (A)</p> Signup and view all the answers

Why do plants in warmer climates tend to produce more saturated fats compared to those in cooler climates?

<p>Plants cannot thermoregulate and produce primarily unsaturated liquid oils but more saturated fats are produced in warmer climates. (C)</p> Signup and view all the answers

Why are unsaturated fatty acids less likely to contribute to the formation of fatty deposits in blood vessels compared to saturated fatty acids?

<p>Unsaturated fatty acids tend to stay liquid at body temperature. (B)</p> Signup and view all the answers

How do trans fatty acids differ structurally from cis-unsaturated fatty acids, and what is the consequence of this difference?

<p><em>Trans</em> fatty acids have hydrogen atoms on opposite sides of the double bond and function more like saturated fats. (B)</p> Signup and view all the answers

Why are trans fats considered particularly unhealthy compared to other types of fats?

<p><em>Trans</em> fats greatly increase blood cholesterol levels. (A)</p> Signup and view all the answers

How does the production of trans fats typically occur?

<p><em>Trans</em> fats are primarily produced via industrial cooking practices. (A)</p> Signup and view all the answers

What are the two key functions of adipose tissue in animals?

<p>Long-term energy storage and thermal insulation (B)</p> Signup and view all the answers

How does blubber, a specialized form of adipose tissue in marine mammals, contribute to their survival in aquatic environments?

<p>Blubber keeps the animal warm and helps to keep it buoyant in water. (B)</p> Signup and view all the answers

Why are triglycerides considered effective as a long-term energy storage molecule?

<p>Triglycerides produceroughly twice as much energy per gram (B)</p> Signup and view all the answers

Which of the following statements accurately describes the roles of estradiol and testosterone?

<p>Estradiol is the main form of estrogen produced by the ovaries, while testosterone is the primary androgen produced by the testes. (B)</p> Signup and view all the answers

Why does continual over-eating lead to the accumulation of adipose tissue?

<p>The body stores excess energy as triglycerides in adipose tissue. (A)</p> Signup and view all the answers

What is the basic structural unit of steroids?

<p>Four-fused candon rings (A)</p> Signup and view all the answers

What characteristic of proteins enables them to perform a wide range of functions within a cell?

<p>Their diverse properties due to varying amino acid sequences and the ability to change expression levels. (A)</p> Signup and view all the answers

How does the non-polar and hydrophobic nature of steroids affect their function in the body?

<p>It enables them to pass through the phospholipid bilayer and find to intracellular receptors (A)</p> Signup and view all the answers

How do protein expression levels adjust to meet cellular requirements?

<p>Genetic instructions are activated or deactivated based on the cell's needs. (A)</p> Signup and view all the answers

Which chemical groups are common to all amino acids?

<p>An amine group, a carboxyl group, and a variable R group. (C)</p> Signup and view all the answers

What is the precursor molecule from which steroid hormones are synthesized?

<p>Cholesterol (D)</p> Signup and view all the answers

Which of the following is an example of a steroid hormone?

<p>Those produced by the gonads. (C)</p> Signup and view all the answers

If an amino acid is described as 'essential,' what does this imply about its availability to an organism?

<p>It must be obtained from the diet because the organism cannot synthesize it. (D)</p> Signup and view all the answers

How do the solubility properties of triglycerides relate to their function as a long-term energy storage molecule?

<p>Triglycerides insoluble in water (much harder to transport) (B)</p> Signup and view all the answers

Under what circumstances might a conditional amino acid become essential?

<p>When the organism's synthesis rate is insufficient to meet its needs. (A)</p> Signup and view all the answers

If a plant species is introduced from a cold climate to a warmer climate, what changes in its lipid composition might be expected over several generations?

<p>An increase in the proportion of saturated fats to maintain membrane fluidity. (C)</p> Signup and view all the answers

Consider a scenario where a cell is deficient in a particular non-essential amino acid. What mechanism would the cell most likely employ to address this deficiency?

<p>Synthesize the amino acid from other available amino acids. (A)</p> Signup and view all the answers

A researcher analyzes a newly discovered organism and finds that its proteins are composed of only 15 different amino acids. What would be a valid conclusion based on this observation?

<p>The organism has a unique genetic code that specifies fewer amino acids than other known organisms. (C)</p> Signup and view all the answers

A patient has a metabolic disorder that impairs their ability to convert one non-essential amino acid into another. How should their diet be modified to address this issue?

<p>Increase intake of the non-essential amino acid they cannot produce. (C)</p> Signup and view all the answers

During periods of intense physical stress, such as recovery from severe burns, the body's demand for certain amino acids increases significantly. How does this change affect the classification of some amino acids?

<p>Non-essential amino acids may become conditional because the body's synthesis rate is insufficient. (D)</p> Signup and view all the answers

How does altering the isoelectric point (pI) of an amino acid typically affect its properties within a protein?

<p>It modifies the amino acid's charge and solubility, potentially affecting protein folding and interactions. (B)</p> Signup and view all the answers

Which of the following best describes the central dogma of molecular biology regarding protein synthesis?

<p>Amino acid sequence determines protein structure, which is encoded by a nucleotide sequence within DNA. (B)</p> Signup and view all the answers

What is the primary role of transcription in protein expression?

<p>Creating an mRNA transcript using a DNA template. (B)</p> Signup and view all the answers

How can alternative splicing increase protein diversity, and why is this important?

<p>Alternative splicing allows a single gene to produce multiple polypeptide variants, which increases proteome diversity beyond what's directly encoded in the genome. (B)</p> Signup and view all the answers

What is the significance of post-translational modifications in protein function?

<p>They promote changes to an amino acid sequence, potentially altering protein activity, localization, or interactions. (A)</p> Signup and view all the answers

Given that the human genome encodes approximately 20,000 proteins, how can the human proteome include approximately 100,000 proteins?

<p>Post-translational modifications and alternative splicing can generate multiple protein variants from a single gene. (D)</p> Signup and view all the answers

How would a mutation affecting the splice sites within a gene most likely impact the resulting protein?

<p>It would lead to the production of a protein with an altered amino acid sequence due to changes in exon inclusion. (A)</p> Signup and view all the answers

Which of the following describes a scenario where a gene does not get translated?

<p>The gene codes for a non-coding RNA molecule with a regulatory function. (A)</p> Signup and view all the answers

What is a 'proteome', and how does it differ from the genome?

<p>The proteome is the total number of proteins expressed within a cell or organism at a given time; the genome is the complete set of genetic instructions. (B)</p> Signup and view all the answers

Considering post-translational modifications, which of the following modifications is most likely to influence protein-protein interactions?

<p>Glycosylation, which can add bulky sugar moieties to the protein surface. (A)</p> Signup and view all the answers

What structural feature distinguishes amylopectin from amylose?

<p>Amylopectin is branched due to $\alpha$-1,6-glycosidic linkages, while amylose is a linear chain with only $\alpha$-1,4-glycosidic linkages. (C)</p> Signup and view all the answers

Which characteristic of cellulose contributes most to its mechanical stability?

<p>The arrangement of $\beta$-glucose monomers leading to linear chains that can form hydrogen bonds between them. (B)</p> Signup and view all the answers

What is the primary function of glycoproteins, in the context of cell recognition?

<p>To serve as surface markers that facilitate cell-cell interactions and recognition. (C)</p> Signup and view all the answers

Which of the following properties is characteristic of non-polar molecules, such as lipids, that defines their interaction with water?

<p>Repulsion by polar molecules, leading to aggregation in aqueous solvents. (C)</p> Signup and view all the answers

What type of reaction is involved in the formation of ester linkages in lipids?

<p>Condensation, which links fatty acids to an alcohol group, releasing water. (C)</p> Signup and view all the answers

How does the structure of phospholipids contribute to their ability to form biological membranes?

<p>They have both polar and non-polar regions, enabling them to form a bilayer structure with the hydrophobic tails facing inward and the hydrophilic heads facing outward. (B)</p> Signup and view all the answers

Which of the following best explains why animals store energy as fats rather than as glycogen?

<p>Fats provide more energy per unit mass compared to glycogen, and they do not require as much water for storage. (B)</p> Signup and view all the answers

How does the presence of double bonds in unsaturated fatty acids affect their physical properties?

<p>They create kinks in the fatty acid chains, preventing them from packing tightly and decreasing the melting point. (C)</p> Signup and view all the answers

How do waxes provide effective water-repelling surfaces in plants and animals?

<p>Waxes are non-polar molecules that prevent water from adhering to the surface. (D)</p> Signup and view all the answers

In the context of lipid classification, how are derived lipids different from simple and compound lipids?

<p>Derived lipids are produced through the hydrolysis of simple and compound lipids. (A)</p> Signup and view all the answers

What is the role of the endoplasmic reticulum and Golgi body in the context of glycoproteins

<p>They facilitate glycosylation, the process of attaching carbohydrates to proteins to form glycoproteins. (B)</p> Signup and view all the answers

What is the significance of 1'-4' and 1'-6' linkages in polysaccharides like starch and glycogen?

<p>1'-4' linkages create the main chain of the polymer, while 1'-6' linkages facilitate branching. (B)</p> Signup and view all the answers

How does the alternating arrangement of $\beta$-glucose monomers affect the structure of cellulose?

<p>It allows for the formation of long, straight chains that can pack tightly together, increasing mechanical stability. (A)</p> Signup and view all the answers

What is the role of thermal insulation in lipids and their properties?

<p>Lipids like fats and oils help maintain body temperature due to their insulating properties (D)</p> Signup and view all the answers

Considering the chemical structure, what makes saturated fatty acids different from unsaturated fatty acids?

<p>Saturated acids contains the max number of hydrogen atoms. (D)</p> Signup and view all the answers

Flashcards

Glucose

A simple sugar and primary energy source for cells.

Isomers

Different compounds with the same molecular formula but different structures.

Monosaccharides

Single sugar molecules that are the building blocks of carbohydrates.

Hydrophilic

Substances that can interact well with water, making them soluble.

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Cellular Respiration

Process by which cells convert glucose into energy (ATP).

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ATP

A molecule that carries energy within cells, made from glucose.

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Glycosidic Bond

A bond that forms between two monosaccharides in a condensation reaction.

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Disaccharide

A sugar molecule composed of two monosaccharides linked together.

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Polysaccharides

Complex carbohydrates formed by linking multiple monosaccharides together.

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Starch

A storage form of glucose in plants, made of long chains of glucose units.

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Saturated Fatty Acids

Fatty acids with no double bonds in their chains; solid at room temperature.

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Melting Point

The temperature at which a substance changes from solid to liquid.

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Intermolecular Forces

Forces that hold molecules together; stronger in saturated fats.

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Unsaturated Fatty Acids

Fatty acids that contain one or more double bonds; typically liquid at room temperature.

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Double Bonds

Chemical bonds where two pairs of electrons are shared; found in unsaturated fats.

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Mono-unsaturated

Fatty acids with one double bond in their structure.

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Polyunsaturated

Fatty acids with multiple double bonds in their chains; more loosely packed.

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Kinked Chains

Structure of unsaturated fatty acids that prevents tight packing; results in liquid form.

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Estradiol

The main form of estrogen produced by ovaries.

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Testosterone

The primary androgen produced by the testes.

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Proteins

Diverse class of organic compounds fulfilling various functions for cells.

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Macromolecule

A large molecule, such as protein, that makes up more than 50% of cell's dry weight.

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Protein Synthesis

Process by which proteins are produced based on genetic instructions from DNA.

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Amino Acids

Building blocks of proteins; 20 common types in living organisms.

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Essential Amino Acids

Cannot be synthesized by the body, must come from diet.

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Non-Essential Amino Acids

Amino acids that can be synthesized by the body.

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Conditional Amino Acids

Amino acids that are essential under certain conditions or times.

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Protein Expression Levels

Change according to the cellular requirements and instructions from DNA.

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Amino Acid Charge

Changing pH alters charge and solubility of amino acids.

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Protein Structure

Protein structure is determined by the amino acid sequence encoded by DNA.

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Gene Expression

Production of proteins involves transcription and translation.

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Transcription

Process where mRNA is synthesized from a DNA template.

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Translation

The process that produces polypeptides from mRNA transcripts.

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Proteome

The total set of proteins expressed in a cell at a given time.

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Alternative Splicing

A process that creates multiple protein variants from a single gene.

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Post-Translational Modifications

Changes made to proteins after translation, affecting their function.

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Polypeptide Variability

Not all genes translate to proteins; some are skipped or modified.

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Human Proteins

The human genome encodes approximately 20,000 proteins, but the proteome can have around 100,000.

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Energy Storage Polymers

Polymers like glycogen and starch used for short-term energy storage.

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Glycogen Structure

Branched polymer of glucose, more branched than amylopectin.

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Starch Types

Includes amylose (helical structure) and amylopectin (branched).

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Cellulose

Polysaccharide made from beta-glucose, forms linear chains.

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Microfibrils

Bundles of cellulose chains providing structural stability.

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Glycoproteins

Proteins with attached carbohydrates via glycosidic linkages.

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Glycosylation

Process of attaching carbohydrates to proteins.

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Lipids

Non-polar molecules used for long-term energy storage.

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Amphipathic Molecules

Molecules with both polar and non-polar regions.

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Triglycerides

Simple lipids made of three fatty acids and glycerol.

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Phospholipids

Lipids with a phosphate group replacing one fatty acid chain.

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Condensation Reaction

Chemical reaction that forms an ester bond in lipids.

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Derived Lipids

Lipids produced from the hydrolysis of other lipids.

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Hydrogen Bonds in Cellulose

Cross-linkages that increase mechanical stability.

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Unsaturated oils

Liquid oils produced by plants; cannot thermoregulate.

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Saturated fats in plants

Plants in warmer climates produce more saturated fats compared to cooler ones.

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Atherosclerosis

Condition caused by fatty deposits in blood vessels leading to high blood pressure.

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Cis vs Trans fatty acids

Cis has hydrogen on the same side; Trans has them on opposite sides of the double bond.

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Trans fats

Unsaturated fats that behave like saturated fats, increasing cholesterol levels.

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Adipose tissue

Tissue in animals that stores triglycerides and maintains thermal insulation.

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Blubber

Thick layer of adipose tissue in marine mammals for warmth and buoyancy.

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Solubility of triglycerides

Triglycerides are insoluble in water, making transport difficult.

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Osmotic pressure

Triglycerides do not exert osmotic pressure due to being hydrophobic.

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Energy yield of triglycerides

Triglycerides produce roughly twice as much energy per gram compared to carbohydrates.

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Steroids

Lipids made of four fused carbon rings, signaling molecules in the body.

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Steroid hormones

Hormones synthesized from cholesterol that pass through cell membranes.

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Intracellular receptors

Receptors inside the cells that bind to steroid hormones.

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Fatty deposits

Clogs in blood vessels from saturated fatty acids, leading to health issues.

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Study Notes

Carbohydrates

  • Made of C, H, and O (typically in the ratio CHâ‚‚O)â‚™
  • Monosaccharides are the repeating monomers, forming simple sugars
  • Monosaccharides form ring structures, which is more energetically favorable
  • Monosaccharides - most have 5 carbons (pentose sugars) or 6 carbons (hexose sugars)
  • Examples of pentoses include ribose and deoxyribose
  • Glucose, a hexose sugar, is a primary energy source in cells.
  • Isomers exist for glucose with different orientations, examples a-glucose and ß-glucose

Energy Source

  • Primary role: chemical energy, often glucose
  • Energy stored in covalent bonds, released via cellular respiration
  • Smaller, polar molecules making them hydrophilic and easy transport between cells
  • Glucose converted to starch or glycogen for storage

Polysaccharides

  • Glycosidic bonds form in condensation reactions, linking monosaccharides
  • Disaccharides: two monosaccharides linked together (e.g., maltose, sucrose, lactose)
  • Polysaccharides can be complexed with other molecules (e.g., glycoproteins)
  • Energy storage polymers:
    • Glycogen (animals)
    • Starch (plants)
      • Amylose: helical, 1-4' linkages
      • Amylopectin: branched, additional 1-6' linkages
  • Structure
    • Cellulose: linear chains of β-glucose with alternating arrangements. Hydrogen bonds increase mechanical stability, forming microfibrils.

Recognition

  • Glycoproteins: proteins with attached carbohydrates via glycosidic linkages.
  • Glycosylation occurs in the endoplasmic reticulum or Golgi body
  • ABO blood types are examples of glycoproteins on red blood cells.

Lipids

  • Nonpolar molecules, some with polar regions (amphipathic)
  • Roles: energy storage, thermal insulation, membrane structure, signaling
  • Properties: nonpolar, low solubility in polar solvents, aggregate in aqueous solvents
  • Types of Lipids
    • Simple lipids:
      • Triglycerides (fats and oils), waxes
    • Compound lipids:
      • Phospholipids, glycolipids
    • Derived lipids:
      • Steroids, prostaglandins
  • Ester linkages: fatty acid chains covalently linked to an alcohol group via condensation reactions
  • Triglycerides: 3 fatty acid chains linked to a glycerol
  • Phospholipids: one fatty acid replaced by a polar phosphate group

Fatty Acids

  • Saturated: no double bonds, straight chains, solid at room temp (higher melting point)
  • Unsaturated: one or more double bonds, kinked chains, liquid at room temp (lower melting point)
  • Fats vs. Oils - based on saturation level and their respective properties
    • Endotherms store more saturated fats
    • Plants in warmer climates store more saturated fats compared to plants in cooler climates

Health Consequences

  • Saturated fats tend to stay solid at high temperatures → more likely to form deposits in blood vessels, leading to atherosclerosis and high blood pressure.
  • Unsaturated fats stay liquid → less likely to clog blood vessels.
  • Trans fats: unsaturated fatty acids with different arrangements, acting more like saturated fats and raising cholesterol levels.
  • Industrial cooking processes produce trans fats.
  • Triglycerides store energy in adipose tissue, providing thermal insulation and buoyancy.

Steroids

  • Derived lipids composed of four fused carbon rings
  • Signaling molecules (hormones): pass through the phospholipid bilayer and bind to intracellular receptors
  • Examples: oestrogen (produced by ovaries) and testosterone (produced by testes)

Proteins

  • Extremely diverse organic compounds, fulfilling a wide range of functions

  • Made of amino acids

  • Amino acids have an amine group, carboxyl group, and variable group.

  • Types of amino acids exist:

    • Essential: body cannot synthesize, must be obtained from the diet.
    • Nonessential: body can synthesize.
    • Conditional: body may synthesize but under certain conditions (e.g., pregnancy).
  • Protein folding: amino acid sequence determines the 3D structure, crucial for function

  • Protein functions include: structure, hormones, immunity, transport, sensitivity, movement and enzymes

  • Denaturation: Change in protein structure due to factors like temperature or pH changes, leading to loss of biological activity.

Proteome

  • Total proteins expressed within a cell or organism at a given time
  • Larger than the totality of genes due to alternative splicing.
  • Protein expression levels change with the requirements of the cell, controlled by genetic instructions.

Nucleic Acids

  • Store genetic instructions and pass them between generations
  • Two types:
    • DNA: Deoxyribonucleic acid (double-stranded)
    • RNA: Ribonucleic acid (single-stranded)
  • Nucleotides: monomers consisting of a pentose sugar, phosphate group and a nitrogenous base
    • Purines (adenine, guanine)
    • Pyrimidines (cytosine, thymine, uracil)
  • DNA and RNA structures: DNA is a double helix , RNA is a single strand. Each have a backbones, which are combinations of sugars and phosphates

###DNA Organization

  • Prokaryotes have circular chromosomes (unpackaged form)
  • Eukaryotes have linear chromosomes with packaged DNA (DNA and histone proteins form chromatin)

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