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Questions and Answers
What is the purpose of using monoclonal antibodies (mAbs) as diagnostic tools?
What is the purpose of using monoclonal antibodies (mAbs) as diagnostic tools?
- To produce hybridoma technology
- To treat bacterial infections
- To stimulate the immune system
- To identify the presence of specific proteins (correct)
How are monoclonal antibodies (mAbs) produced by hybridoma technology?
How are monoclonal antibodies (mAbs) produced by hybridoma technology?
- By injecting mice with antigens
- By directly extracting them from animals that have been immunized
- By transforming human B-cells with Epstein-Barr virus
- By fusing un-dividing B lymphocytes producing specific antibodies with myeloma cells (correct)
Which method is an alternative way to produce monoclonal antibodies using human B-cells?
Which method is an alternative way to produce monoclonal antibodies using human B-cells?
- By transforming human B-cells with Epstein-Barr virus (correct)
- By injecting mice with antigens
- By directly extracting them from animals that have been immunized
- By fusing B lymphocytes with myeloma cells
What is the basis of hybridoma technology for producing monoclonal antibodies?
What is the basis of hybridoma technology for producing monoclonal antibodies?
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What is the main benefit of using monoclonal antibodies (mAbs) for therapeutic purposes?
What is the main benefit of using monoclonal antibodies (mAbs) for therapeutic purposes?
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Study Notes
Antibody Types
- Polyclonal Antibodies: Generated from the immune response to an antigen injected into an animal, leading to a mix of antibodies from multiple lymphocyte clones.
- Polyclonal Antisera: Serum collected post-immunization that contains a diverse range of antibodies that target the original antigen.
Monoclonal Antibodies
- Defined as antibodies produced by a single clone of B-cells, resulting in identical heavy and light chain structures.
- Advantages:
- High specificity towards the target antigen.
- More consistent efficacy in research and therapeutic applications.
- Predictable toxicity profiles in vivo.
Historical Development
- Monoclonal antibodies (mAbs) were first created in 1970.
- Hybridoma Technology: This method enabled the expansion and immortalization of individual B-cell clones, establishing a foundation for producing mAbs.
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Description
Learn about the differences between monoclonal and polyclonal antibodies and how they are produced in the immune response. Understand the enrichment process of antibodies specific to antigens.
