Monoclonal Antibodies in Medicine
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Questions and Answers

What substance is used to facilitate the fusion of myeloma cells with immune spleen cells?

  • Freund's adjuvant
  • Polyethylene glycol (PEG) (correct)
  • Monoclonal antibodies
  • Thymidine

What type of medium is used to select for hybridoma cells after fusion?

  • HAT medium (correct)
  • RPMI 1640
  • LB broth
  • DMEM

Which method can be used for cloning hybridoma cell lines?

  • Polymerase chain reaction
  • Injection into mouse peritoneal cavity (correct)
  • Thermal cycling
  • Optical imaging

What is a common diagnostic application of monoclonal antibodies?

<p>Blood typing (B)</p> Signup and view all the answers

Chimeric antibodies are beneficial because they contain which type of portion?

<p>Human Fc portion (D)</p> Signup and view all the answers

What role do monoclonal antibodies play in cancer treatment?

<p>Coupling with radioactive atoms to kill cancer cells (A)</p> Signup and view all the answers

Which of the following is an incorrect application of monoclonal antibodies?

<p>Enhancing the immune response through vaccines (B)</p> Signup and view all the answers

What is a step involved in ensuring sufficient immune response in mice before hybridoma cell production?

<p>Measuring serum antibody titers (A)</p> Signup and view all the answers

Flashcards

Monoclonal Antibody

A type of antibody produced by a single clone of B cells, ensuring all antibodies are identical and specific for a single epitope.

Hybridoma Technology

The process of fusing an immortal myeloma cell line with an antibody-producing B cell from an immunized animal to create a hybrid cell that continuously produces a specific antibody.

HAT Medium

A medium containing hypoxanthine, aminopterin, and thymidine used to select for hybridoma cells.

Immunofluorescence

A technique used to identify and enumerate different types of immune cells based on their surface markers using specific monoclonal antibodies.

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Radioimmunotherapy

The process of using monoclonal antibodies conjugated with radioactive isotopes to deliver targeted radiation to specific cells, like cancer cells.

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Chimeric Antibodies

Antibodies with a human Fc region and a non-human variable region, minimizing the risk of immune rejection in humans.

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mAb (monoclonal antibody)

A type of specific antibody used in diagnostics, research, and treatment, created through hybridoma technology.

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Ascites Production

A technique used for generating monoclonal antibodies by injecting the hybridoma cells into the peritoneal cavity of a mouse, resulting in the production of ascites fluid containing high concentrations of the antibody.

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