Podcast
Questions and Answers
What is the most abundant di-nucleotide repeat motif in plants?
What is the most abundant di-nucleotide repeat motif in plants?
What is the main reason why AT-enrichment methods have not been developed for SSR markers?
What is the main reason why AT-enrichment methods have not been developed for SSR markers?
What makes SSR markers perfectly suited for use in map-based cloning?
What makes SSR markers perfectly suited for use in map-based cloning?
What distinguishes the polymorphisms of SSRs based on the length of PCR products?
What distinguishes the polymorphisms of SSRs based on the length of PCR products?
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What type of DNA polymorphisms result from differences in the number of copies of a DNA sequence repeated many times in tandem at a particular site in a chromosome?
What type of DNA polymorphisms result from differences in the number of copies of a DNA sequence repeated many times in tandem at a particular site in a chromosome?
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Which molecular marker technique involves the PCR amplification of genomic restriction fragments generated by specific restriction enzymes and oligonucleotide adapters?
Which molecular marker technique involves the PCR amplification of genomic restriction fragments generated by specific restriction enzymes and oligonucleotide adapters?
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In AFLP, what is used to ligate to the ends of the DNA fragments?
In AFLP, what is used to ligate to the ends of the DNA fragments?
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What is the purpose of AFLP in molecular breeding?
What is the purpose of AFLP in molecular breeding?
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Which restriction enzymes are generally used in AFLP for digesting genomic DNA?
Which restriction enzymes are generally used in AFLP for digesting genomic DNA?
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What makes AFLP a DNA fingerprinting technique?
What makes AFLP a DNA fingerprinting technique?
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Study Notes
Di-Nucleotide Repeat Motif in Plants
- The most abundant di-nucleotide repeat motif in plants is (AT) n, indicating a high frequency of adenine and thymine pairs.
AT-Enrichment and SSR Markers
- Lack of AT-enrichment methods for SSR markers primarily stems from the difficulty in isolating sequences with high adenine-thymine content effectively due to their repetitive nature.
Suitability of SSR Markers in Map-Based Cloning
- SSR markers are ideal for map-based cloning due to their high polymorphism levels, co-dominant inheritance, and ability to amplify products of variable lengths for precise mapping.
Polymorphisms in SSRs
- SSR polymorphisms can be distinguished based on the length of PCR products; variations in length correspond to differences in the number of repeat units in the DNA sequence.
DNA Polymorphisms from Tandem Repeats
- DNA polymorphisms arise from differences in the number of tandem repeated DNA sequences at a specific locus on a chromosome, leading to variability in genomic structure.
AFLP Molecular Marker Technique
- AFLP employs PCR amplification of genomic restriction fragments created by specified restriction enzymes, combined with selected oligonucleotide adapters.
Ligating DNA Fragments in AFLP
- Specific adaptors are used to ligate to the ends of the DNA fragments in AFLP, allowing for subsequent amplification and analysis.
Purpose of AFLP in Molecular Breeding
- AFLP is utilized in molecular breeding for genetic diversity assessment, marker-assisted selection, and creation of genetic maps.
Restriction Enzymes Used in AFLP
- Typically, restriction enzymes used in AFLP for digesting genomic DNA include EcoRI and MseI, chosen for their ability to cut at specific sequences.
DNA Fingerprinting with AFLP
- AFLP acts as a DNA fingerprinting technique due to its capacity to produce a unique pattern of bands based on the fragment sizes of amplified products, reflecting genetic variation.
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Description
Test your knowledge about molecular markers and their types used in molecular breeding. Learn about RFLP, PCR-based markers like RAPD, AFLP, SSR, and SNP. Explore the applications of AFLP in molecular breeding.
