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Questions and Answers
What are the intended learning outcomes at the end of the session?
Adhere to personal health and safety practices, maintain a clean and organized workspace, disinfect and dispose of infectious materials, take appropriate actions following accidental exposure to potentially infectious specimens, follow written safety procedures and keep proper safety records.
Which of the following is considered a process of decontamination?
Hand dryers are recommended for drying hands after washing in the laboratory.
False
The most common concentration of Sodium Hypochlorite typically bought is ____.
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What is the recommended contact time for Sodium Hypochlorite for general surface decontamination?
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What should never be done in the laboratory according to safety practices?
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What are the potential routes of exposure to biological waste?
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Ultraviolet light can effectively destroy all microorganisms.
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What is the primary role of personal protective equipment in a laboratory?
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In the event of a spill or splash, the first step is to ____ the room.
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Match the cleaning agents to their functions:
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Study Notes
Lesson Intended Learning Outcomes (LILO)
- Adhere to personal health and safety practices in the laboratory.
- Maintain a workspace that is organized and clean.
- Properly disinfect and dispose of infectious materials.
- Follow protocols following accidental exposure to potentially infectious specimens.
- Comply with written safety procedures and maintain safety records.
Laboratory Quality System
- Includes organization, personnel management, equipment, stock management, quality control, data management, SOPs, and occurrence assessment.
- Emphasizes process improvement and the management of safety and waste in the laboratory.
Importance of Safety
- Contact with human blood or hazardous chemicals can pose severe risks.
- Safety measures protect against infection, injury, and poisoning.
Hand Hygiene
- Required to wash hands after handling hazardous materials and before leaving the laboratory.
- Handwashing process includes lathering for 20 seconds, rinsing for 10 seconds, and total duration of 40-60 seconds.
Decontamination Processes
- Decontamination: Removal or neutralization of biological agents to eliminate hazards.
- Sterilization: Complete removal or destruction of all forms of life including spores.
- Disinfection: Use of chemical agents to inhibit or kill microorganisms on inanimate objects.
- Antisepsis: Application of chemicals to the skin to prevent infection.
Standard Precautions
- Treat all specimens as potentially infectious.
- Avoid touching the face with gloved or unwashed hands.
- Keep work areas clean and organized and avoid unnecessary items.
Decontamination/Disinfection Agents
- Effective against enveloped RNA viruses include 70% ethyl alcohol and sodium hypochlorite at specific concentrations.
- Ensure contact time for bleach is 10-15 minutes for effective decontamination.
Chemical Agents and Efficacy
- Efficacy influenced by factors like organic and microbial load, type of organism, and disinfectant characteristics.
Sodium Hypochlorite
- Common concentrations range from 5-8.25% with important preparation ratios for use.
- Contact time critical for effective decontamination; avoid exposure to air and direct sunlight.
Alcohol
- Preferred concentration: 60-90%, enhances contact time for effectiveness.
- Should be allowed to evaporate; ineffective alone for molecular testing.
Ultraviolet Light
- Effective in damaging DNA; maximum lethality at 260nm wavelength.
- Only effective on surfaces directly exposed to UV light.
Lysol Disinfection
- Active ingredients like Benzalkonium Chloride and Hydrogen Peroxide effectively target microorganisms.
Prion Inactivation
- Requires immersion in alkaline solutions followed by autoclaving for effective deactivation.
Autoclaving
- Utilizes steam under pressure to sterilize; Biological indicators confirm effectiveness (e.g., Geobacillus stearothermophilus).
Biosafety Cabinet
- Must be calibrated and certified for use; provides protection through HEPA filters.
- Ensures containment and protection of both personnel and environment.
Biosafety Management
- Development of plans and training programs essential for laboratory personnel safety.
Health and Medical Surveillance
- Immunization, early detection for infections, and exclusion of susceptible individuals are vital.
Safe Work Practices
- Fundamental practices include handwashing, proper PPE use, and immediate disposal of hazardous waste.
Biological Waste Disposal
- Hazardous exposures can include blood, swabs, tissues, and other body fluids.
- Proper inactivation methods are necessary for safe disposal.
Chemical Hazards Management
- Requires label verification, use of PPE, and safe handling techniques including proper disposal of spills.
Carcinogen Awareness
- Includes various laboratory agents like Acetic Acid and Ethidium Bromide; caution is needed due to potential health impacts.
Spill and Accident Protocols
- Immediate evacuation of area, proper cleaning methods, and reporting protocols.
Definitions in Biosafety
- Distinguishes between disinfectants, antiseptics, microbicides, and other relevant terms for effective laboratory practices.
Action Plan for Safety
- Identify hazards, establish safety policies, conduct training, and perform regular audits for safety management.
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Description
Learn about personal health and safety practices, maintaining a clean workspace, disinfecting and disposing of infectious materials in a molecular laboratory. This quiz is based on a lesson from Our Lady of Fatima University.
