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Questions and Answers
What is the main purpose of gel electrophoresis?
What is the main purpose of gel electrophoresis?
Which technique uses an antibody linked to an enzyme to quantify the amount of a molecule?
Which technique uses an antibody linked to an enzyme to quantify the amount of a molecule?
What is the fundamental importance of PCR in genetic testing?
What is the fundamental importance of PCR in genetic testing?
Which process involves the use of allele-specific tests?
Which process involves the use of allele-specific tests?
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What is the function of an antibody in diagnostic tests?
What is the function of an antibody in diagnostic tests?
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Study Notes
Gel Electrophoresis
- Main purpose is to separate charged biological molecules, such as DNA, RNA, and proteins, based on size and charge.
- Utilizes an electric field to pull molecules through a gel matrix, creating distinct bands for analysis.
Enzyme-Linked Immunosorbent Assay (ELISA)
- Technique that employs antibodies linked to an enzyme for detection and quantification of specific molecules, often proteins.
- Commonly used in various fields, including clinical diagnostics and research, for measuring concentrations of antigens or antibodies.
Polymerase Chain Reaction (PCR)
- Crucial for amplifying small segments of DNA, making it easier to analyze genetic material.
- Essential in genetic testing for identifying genetic mutations, pathogens, or for forensic analysis.
Allele-Specific Testing
- Involves using tests that can differentiate between alleles, helping to detect specific genetic variants associated with diseases.
- Utilizes techniques like PCR or hybridization to assess the presence of particular alleles in a sample.
Antibody Function in Diagnostics
- Antibodies serve as specific binding agents for target antigens in diagnostic tests, allowing for the identification of diseases or conditions.
- Their specificity enables the development of tests that provide reliable results in detecting infections or autoimmune disorders.
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Description
Learn about molecular processes, gene cloning, restriction analysis, DNA sequencing, and DNA electrophoresis in this session by Dr. Ibrahim Naqid at University of Zakho Zakho Medical College.
