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Questions and Answers
What is the main purpose of gel electrophoresis?
Which technique uses an antibody linked to an enzyme to quantify the amount of a molecule?
What is the fundamental importance of PCR in genetic testing?
Which process involves the use of allele-specific tests?
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What is the function of an antibody in diagnostic tests?
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Study Notes
Gel Electrophoresis
- Main purpose is to separate charged biological molecules, such as DNA, RNA, and proteins, based on size and charge.
- Utilizes an electric field to pull molecules through a gel matrix, creating distinct bands for analysis.
Enzyme-Linked Immunosorbent Assay (ELISA)
- Technique that employs antibodies linked to an enzyme for detection and quantification of specific molecules, often proteins.
- Commonly used in various fields, including clinical diagnostics and research, for measuring concentrations of antigens or antibodies.
Polymerase Chain Reaction (PCR)
- Crucial for amplifying small segments of DNA, making it easier to analyze genetic material.
- Essential in genetic testing for identifying genetic mutations, pathogens, or for forensic analysis.
Allele-Specific Testing
- Involves using tests that can differentiate between alleles, helping to detect specific genetic variants associated with diseases.
- Utilizes techniques like PCR or hybridization to assess the presence of particular alleles in a sample.
Antibody Function in Diagnostics
- Antibodies serve as specific binding agents for target antigens in diagnostic tests, allowing for the identification of diseases or conditions.
- Their specificity enables the development of tests that provide reliable results in detecting infections or autoimmune disorders.
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Description
Learn about molecular processes, gene cloning, restriction analysis, DNA sequencing, and DNA electrophoresis in this session by Dr. Ibrahim Naqid at University of Zakho Zakho Medical College.
