Molecular Biology and Diagnostics Review

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Who is credited with introducing the term 'molecular disease' in the medical vocabulary?

Gregor Mendel

James Watson

Francis Crick

Linus Pauling (correct)

Who isolated genetic material in 1869?

James Watson

Gregor Mendel

Johann Friedrich Miescher (correct)

Francis Crick

What is the product of transcription?

RNA (correct)

DNA

mRNA

rRNA

Who established the fundamental laws of heredity or inheritance?

Gregor Mendel Signup and view all the answers

Who discovered the three-dimensional structure of DNA?

James Watson and Francis Crick Signup and view all the answers

In what year did Johann Friedrich Miescher begin his experiment to isolate genetic material?

1866 Signup and view all the answers

What is necessary to generate primers for selective amplification of a target sequence?

Prior knowledge about the target sequence Signup and view all the answers

What can occur as a result of PCR errors?

Mutations in PCR fragments Signup and view all the answers

How should nasopharyngeal specimens be collected?

Through the mouth and sweeping the area above the uvula Signup and view all the answers

Why is RNA isolation more challenging than DNA isolation?

RNA is very unstable and sensitive to external factors Signup and view all the answers

What should be used during RNA extraction to prevent RNA degradation?

RNase-free or nuclease-free solutions Signup and view all the answers

What is the most important step in the laboratory diagnosis of infectious diseases?

Proper specimen collection Signup and view all the answers

What is the primary function of Personal Protective Equipment in a laboratory setting?

To protect against hazards in the laboratory Signup and view all the answers

Which of the following equipment is used to measure the absorption of light by a chemical substance?

Spectrophotometer Signup and view all the answers

What is the term for the process of continually improving the biorisk management system?

Performance Signup and view all the answers

What is the temperature range of a -20oC Freezer?

Below -20oC Signup and view all the answers

What is the term for process and activities that have been shown to be effective in reducing risks?

Practices and Procedures Signup and view all the answers

Which bodily fluid is NOT typically collected for Epstein-Barr Virus PCR amplification?

Urine Signup and view all the answers

What does PCR provide information about in an organism?

The species to which bacteria are most closely related to Signup and view all the answers

Which primer is specific for the detection of Brugia malayi?

HhA1 Signup and view all the answers

On what gene sequence is the MASPCR method based for differentiating Cryptosporidium Genotype 1 and 2?

Hha1 gene sequence Signup and view all the answers

Which Plasmodium species produces Histidine-rich protein?

Plasmodium falciparum Signup and view all the answers

What is detected by the OptiMAL Assay and Diamed Optimal IT?

PARASITIC LDH present in viable malarial parasites Signup and view all the answers

What is the primary concern of Laboratory Biosafety?

Protecting people from harmful biological agents Signup and view all the answers

What is the term for the introduction of a substance into the body through a puncture wound?

Inoculation Signup and view all the answers

Who is known for accidentally pricking his finger with a modified version of the smallpox virus?

Alasdair Pennycook Signup and view all the answers

What is the primary goal of Physical Security in Biosecurity?

Assuring safety from physical intrusion Signup and view all the answers

What is the definition of Biorisk?

The effect of uncertainty expressed by the combination of the consequences of an event and the associated likelihood of occurrence Signup and view all the answers

Who is known for contaminating pastries with Shigella dysentariae and giving them to hospital workers?

Diane Thompson Signup and view all the answers

What type of hazard is related to poor posture and repetitive tasks?

Ergonomic hazard Signup and view all the answers

What is the purpose of prioritizing hazards based on their level of risk?

To allocate resources effectively Signup and view all the answers

Which hazard identification technique involves breaking down tasks into steps to identify hazards?

Job Safety Analysis (JSA) Signup and view all the answers