Molecular Biology and Diagnostics Review
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Questions and Answers

Who is credited with introducing the term 'molecular disease' in the medical vocabulary?

  • Gregor Mendel
  • James Watson
  • Francis Crick
  • Linus Pauling (correct)
  • Who isolated genetic material in 1869?

  • James Watson
  • Gregor Mendel
  • Johann Friedrich Miescher (correct)
  • Francis Crick
  • What is the product of transcription?

  • RNA (correct)
  • DNA
  • mRNA
  • rRNA
  • Who established the fundamental laws of heredity or inheritance?

    <p>Gregor Mendel</p> Signup and view all the answers

    Who discovered the three-dimensional structure of DNA?

    <p>James Watson and Francis Crick</p> Signup and view all the answers

    In what year did Johann Friedrich Miescher begin his experiment to isolate genetic material?

    <p>1866</p> Signup and view all the answers

    What is necessary to generate primers for selective amplification of a target sequence?

    <p>Prior knowledge about the target sequence</p> Signup and view all the answers

    What can occur as a result of PCR errors?

    <p>Mutations in PCR fragments</p> Signup and view all the answers

    How should nasopharyngeal specimens be collected?

    <p>Through the mouth and sweeping the area above the uvula</p> Signup and view all the answers

    Why is RNA isolation more challenging than DNA isolation?

    <p>RNA is very unstable and sensitive to external factors</p> Signup and view all the answers

    What should be used during RNA extraction to prevent RNA degradation?

    <p>RNase-free or nuclease-free solutions</p> Signup and view all the answers

    What is the most important step in the laboratory diagnosis of infectious diseases?

    <p>Proper specimen collection</p> Signup and view all the answers

    What is the primary function of Personal Protective Equipment in a laboratory setting?

    <p>To protect against hazards in the laboratory</p> Signup and view all the answers

    Which of the following equipment is used to measure the absorption of light by a chemical substance?

    <p>Spectrophotometer</p> Signup and view all the answers

    What is the term for the process of continually improving the biorisk management system?

    <p>Performance</p> Signup and view all the answers

    What is the temperature range of a -20oC Freezer?

    <p>Below -20oC</p> Signup and view all the answers

    What is the term for process and activities that have been shown to be effective in reducing risks?

    <p>Practices and Procedures</p> Signup and view all the answers

    Which bodily fluid is NOT typically collected for Epstein-Barr Virus PCR amplification?

    <p>Urine</p> Signup and view all the answers

    What does PCR provide information about in an organism?

    <p>The species to which bacteria are most closely related to</p> Signup and view all the answers

    Which primer is specific for the detection of Brugia malayi?

    <p>HhA1</p> Signup and view all the answers

    On what gene sequence is the MASPCR method based for differentiating Cryptosporidium Genotype 1 and 2?

    <p>Hha1 gene sequence</p> Signup and view all the answers

    Which Plasmodium species produces Histidine-rich protein?

    <p>Plasmodium falciparum</p> Signup and view all the answers

    What is detected by the OptiMAL Assay and Diamed Optimal IT?

    <p>PARASITIC LDH present in viable malarial parasites</p> Signup and view all the answers

    What is the primary concern of Laboratory Biosafety?

    <p>Protecting people from harmful biological agents</p> Signup and view all the answers

    What is the term for the introduction of a substance into the body through a puncture wound?

    <p>Inoculation</p> Signup and view all the answers

    Who is known for accidentally pricking his finger with a modified version of the smallpox virus?

    <p>Alasdair Pennycook</p> Signup and view all the answers

    What is the primary goal of Physical Security in Biosecurity?

    <p>Assuring safety from physical intrusion</p> Signup and view all the answers

    What is the definition of Biorisk?

    <p>The effect of uncertainty expressed by the combination of the consequences of an event and the associated likelihood of occurrence</p> Signup and view all the answers

    Who is known for contaminating pastries with Shigella dysentariae and giving them to hospital workers?

    <p>Diane Thompson</p> Signup and view all the answers

    What type of hazard is related to poor posture and repetitive tasks?

    <p>Ergonomic hazard</p> Signup and view all the answers

    What is the purpose of prioritizing hazards based on their level of risk?

    <p>To allocate resources effectively</p> Signup and view all the answers

    Which hazard identification technique involves breaking down tasks into steps to identify hazards?

    <p>Job Safety Analysis (JSA)</p> Signup and view all the answers

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