Molecular Biology and Diagnostics Review
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Questions and Answers

Who is credited with introducing the term 'molecular disease' in the medical vocabulary?

  • Gregor Mendel
  • James Watson
  • Francis Crick
  • Linus Pauling (correct)

Who isolated genetic material in 1869?

  • James Watson
  • Gregor Mendel
  • Johann Friedrich Miescher (correct)
  • Francis Crick

What is the product of transcription?

  • RNA (correct)
  • DNA
  • mRNA
  • rRNA

Who established the fundamental laws of heredity or inheritance?

<p>Gregor Mendel (C)</p> Signup and view all the answers

Who discovered the three-dimensional structure of DNA?

<p>James Watson and Francis Crick (A)</p> Signup and view all the answers

In what year did Johann Friedrich Miescher begin his experiment to isolate genetic material?

<p>1866 (B)</p> Signup and view all the answers

What is necessary to generate primers for selective amplification of a target sequence?

<p>Prior knowledge about the target sequence (D)</p> Signup and view all the answers

What can occur as a result of PCR errors?

<p>Mutations in PCR fragments (D)</p> Signup and view all the answers

How should nasopharyngeal specimens be collected?

<p>Through the mouth and sweeping the area above the uvula (D)</p> Signup and view all the answers

Why is RNA isolation more challenging than DNA isolation?

<p>RNA is very unstable and sensitive to external factors (C)</p> Signup and view all the answers

What should be used during RNA extraction to prevent RNA degradation?

<p>RNase-free or nuclease-free solutions (A)</p> Signup and view all the answers

What is the most important step in the laboratory diagnosis of infectious diseases?

<p>Proper specimen collection (A)</p> Signup and view all the answers

What is the primary function of Personal Protective Equipment in a laboratory setting?

<p>To protect against hazards in the laboratory (C)</p> Signup and view all the answers

Which of the following equipment is used to measure the absorption of light by a chemical substance?

<p>Spectrophotometer (A)</p> Signup and view all the answers

What is the term for the process of continually improving the biorisk management system?

<p>Performance (C)</p> Signup and view all the answers

What is the temperature range of a -20oC Freezer?

<p>Below -20oC (C)</p> Signup and view all the answers

What is the term for process and activities that have been shown to be effective in reducing risks?

<p>Practices and Procedures (D)</p> Signup and view all the answers

Which bodily fluid is NOT typically collected for Epstein-Barr Virus PCR amplification?

<p>Urine (A)</p> Signup and view all the answers

What does PCR provide information about in an organism?

<p>The species to which bacteria are most closely related to (B)</p> Signup and view all the answers

Which primer is specific for the detection of Brugia malayi?

<p>HhA1 (D)</p> Signup and view all the answers

On what gene sequence is the MASPCR method based for differentiating Cryptosporidium Genotype 1 and 2?

<p>Hha1 gene sequence (C)</p> Signup and view all the answers

Which Plasmodium species produces Histidine-rich protein?

<p>Plasmodium falciparum (C)</p> Signup and view all the answers

What is detected by the OptiMAL Assay and Diamed Optimal IT?

<p>PARASITIC LDH present in viable malarial parasites (B)</p> Signup and view all the answers

What is the primary concern of Laboratory Biosafety?

<p>Protecting people from harmful biological agents (D)</p> Signup and view all the answers

What is the term for the introduction of a substance into the body through a puncture wound?

<p>Inoculation (D)</p> Signup and view all the answers

Who is known for accidentally pricking his finger with a modified version of the smallpox virus?

<p>Alasdair Pennycook (D)</p> Signup and view all the answers

What is the primary goal of Physical Security in Biosecurity?

<p>Assuring safety from physical intrusion (A)</p> Signup and view all the answers

What is the definition of Biorisk?

<p>The effect of uncertainty expressed by the combination of the consequences of an event and the associated likelihood of occurrence (C)</p> Signup and view all the answers

Who is known for contaminating pastries with Shigella dysentariae and giving them to hospital workers?

<p>Diane Thompson (D)</p> Signup and view all the answers

What type of hazard is related to poor posture and repetitive tasks?

<p>Ergonomic hazard (A)</p> Signup and view all the answers

What is the purpose of prioritizing hazards based on their level of risk?

<p>To allocate resources effectively (B)</p> Signup and view all the answers

Which hazard identification technique involves breaking down tasks into steps to identify hazards?

<p>Job Safety Analysis (JSA) (B)</p> Signup and view all the answers

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