Podcast
Questions and Answers
What is the name of the scientist who invented the polymerase chain reaction (PCR) method?
What is the name of the scientist who invented the polymerase chain reaction (PCR) method?
- Kary B. Mullis (correct)
- Buckingham
- La Jolla
- Taq polymerase
What is the primary function of the primers in PCR?
What is the primary function of the primers in PCR?
- To denature the DNA template
- To extend the binding site of the opposite primer
- To bind to the complementary strand of the target fragment (correct)
- To synthesize new strands of DNA
What is the purpose of heating the DNA template in PCR?
What is the purpose of heating the DNA template in PCR?
- To extend the binding site of the primer
- To cool the reaction mixture
- To denature the DNA template (correct)
- To promote primer annealing
What is the result of the PCR process?
What is the result of the PCR process?
What is the name of the enzyme used in PCR?
What is the name of the enzyme used in PCR?
What is the advantage of PCR technology?
What is the advantage of PCR technology?
What is target amplification similar to?
What is target amplification similar to?
What is the advantage of target amplification over cell culture?
What is the advantage of target amplification over cell culture?
What is the first and most used method for amplifying target nucleic acid?
What is the first and most used method for amplifying target nucleic acid?
Who won the Nobel Prize in Chemistry in 1993?
Who won the Nobel Prize in Chemistry in 1993?
What will be discussed in this topic?
What will be discussed in this topic?
What is the purpose of this topic?
What is the purpose of this topic?
What is the result of repeating the steps of denaturation, annealing, and extension in PCR?
What is the result of repeating the steps of denaturation, annealing, and extension in PCR?
What is the temperature range for the annealing step in PCR?
What is the temperature range for the annealing step in PCR?
What is the purpose of the denaturation step in PCR?
What is the purpose of the denaturation step in PCR?
What is the optimal temperature range for DNA polymerase to act in PCR?
What is the optimal temperature range for DNA polymerase to act in PCR?
What is an important parameter in PCR primer design?
What is an important parameter in PCR primer design?
What is the formula for the number of PCR product copies?
What is the formula for the number of PCR product copies?
What is a major advantage of multiplex PCR over singleplex PCR?
What is a major advantage of multiplex PCR over singleplex PCR?
What is the primary purpose of using sequence-specific PCR?
What is the primary purpose of using sequence-specific PCR?
What is a common problem encountered during multiplex PCR?
What is a common problem encountered during multiplex PCR?
What is the primary application of multiplex PCR in a clinical molecular laboratory?
What is the primary application of multiplex PCR in a clinical molecular laboratory?
Who first reported multiplex PCR in 1988?
Who first reported multiplex PCR in 1988?
What type of PCR involves using RNA templates?
What type of PCR involves using RNA templates?
What is the main difference between real-time PCR and conventional PCR?
What is the main difference between real-time PCR and conventional PCR?
What does the CT (threshold cycle) parameter represent?
What does the CT (threshold cycle) parameter represent?
What is the advantage of real-time PCR quantitation?
What is the advantage of real-time PCR quantitation?
What does the fluorescence threshold signify?
What does the fluorescence threshold signify?
What is the correlation between the starting copy number of the nucleic acid target and the fluorescence increase?
What is the correlation between the starting copy number of the nucleic acid target and the fluorescence increase?
What is the purpose of setting a fixed fluorescence threshold?
What is the purpose of setting a fixed fluorescence threshold?
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