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Molecular Biology: Restriction Enzymes Digestion
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Molecular Biology: Restriction Enzymes Digestion

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Questions and Answers

What is the total length of the DNA fragment used in the restriction mapping example?

  • 18 kb
  • 20 kb
  • 15 kb (correct)
  • 12 kb
  • Which enzymes are involved in the restriction mapping example?

  • EcoRI, PstI, HindIII
  • BamHI, NotI, PstI
  • BamHI, EcoRI, HindIII
  • BamHI, EcoRI, PstI (correct)
  • What technique is used to determine the relative locations of restriction enzyme sites?

  • Gel electrophoresis
  • Polymerase chain reaction
  • DNA sequencing
  • Restriction mapping (correct)
  • What is the purpose of performing single enzyme reactions in this restriction mapping example?

    <p>To confirm the presence of a single cutting site for each enzyme</p> Signup and view all the answers

    Which enzyme is mentioned as providing the joining function in recombinant DNA production?

    <p>DNA ligase</p> Signup and view all the answers

    What method is used to analyze the sizes of the DNA fragments produced in the digestion?

    <p>Agarose gel electrophoresis</p> Signup and view all the answers

    What is confirmed by performing a triple digest in the restriction mapping process?

    <p>The locations of all cutting sites</p> Signup and view all the answers

    How are the restriction enzymes and DNA ligase related in the context of recombinant DNA molecules?

    <p>One cuts DNA, and the other joins DNA fragments</p> Signup and view all the answers

    What do restriction endonucleases do?

    <p>Cut within a DNA strand</p> Signup and view all the answers

    Which fragments are yielded from a 15 kb fragment when cut with BamHI?

    <p>14 and 1 kb</p> Signup and view all the answers

    Which option shows the correct fragments yielded from double digestion with BamHI and EcoRI?

    <p>11, 3, and 1 kb</p> Signup and view all the answers

    What type of enzyme is deoxyribonuclease I (DNase I)?

    <p>Endonuclease</p> Signup and view all the answers

    Which enzyme degrades DNA from the termini of the molecule?

    <p>Exonuclease</p> Signup and view all the answers

    What is the primary function of ribonucleases (RNAases)?

    <p>Acting on RNA</p> Signup and view all the answers

    Which enzymes is NOT classified as an exonuclease?

    <p>Si-nuclease</p> Signup and view all the answers

    Which of the following enzymes provides strategies for attacking DNA?

    <p>All of these</p> Signup and view all the answers

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