Molecular Biology: Restriction Enzymes Digestion

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What is the total length of the DNA fragment used in the restriction mapping example?

18 kb

20 kb

15 kb (correct)

12 kb

Which enzymes are involved in the restriction mapping example?

EcoRI, PstI, HindIII

BamHI, NotI, PstI

BamHI, EcoRI, HindIII

BamHI, EcoRI, PstI (correct)

What technique is used to determine the relative locations of restriction enzyme sites?

Gel electrophoresis

Polymerase chain reaction

DNA sequencing

Restriction mapping (correct)

What is the purpose of performing single enzyme reactions in this restriction mapping example?

To confirm the presence of a single cutting site for each enzyme Signup and view all the answers

Which enzyme is mentioned as providing the joining function in recombinant DNA production?

DNA ligase Signup and view all the answers

What method is used to analyze the sizes of the DNA fragments produced in the digestion?

Agarose gel electrophoresis Signup and view all the answers

What is confirmed by performing a triple digest in the restriction mapping process?

The locations of all cutting sites Signup and view all the answers

How are the restriction enzymes and DNA ligase related in the context of recombinant DNA molecules?

One cuts DNA, and the other joins DNA fragments Signup and view all the answers

What do restriction endonucleases do?

Cut within a DNA strand Signup and view all the answers

Which fragments are yielded from a 15 kb fragment when cut with BamHI?

14 and 1 kb Signup and view all the answers

Which option shows the correct fragments yielded from double digestion with BamHI and EcoRI?

11, 3, and 1 kb Signup and view all the answers

What type of enzyme is deoxyribonuclease I (DNase I)?

Endonuclease Signup and view all the answers

Which enzyme degrades DNA from the termini of the molecule?

Exonuclease Signup and view all the answers

What is the primary function of ribonucleases (RNAases)?

Acting on RNA Signup and view all the answers

Which enzymes is NOT classified as an exonuclease?

Si-nuclease Signup and view all the answers

Which of the following enzymes provides strategies for attacking DNA?

All of these Signup and view all the answers

Molecular Biology: Restriction Enzymes Digestion

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