Restriction Enzymes and DNA
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Questions and Answers

What was the year when the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated?

  • 1960
  • 1963 (correct)
  • 1968
  • 1965
  • What is the function of the first enzyme mentioned in the passage?

  • Cuts DNA
  • Replicates DNA
  • Adds methyl groups to DNA (correct)
  • Transcribes DNA
  • How many restriction enzymes have been isolated from bacteria as of the passage?

  • More than 1500
  • More than 900 (correct)
  • More than 500
  • More than 1000
  • What is the recognition sequence of Hind II?

    <p>A specific sequence of six base pairs</p> Signup and view all the answers

    What is the origin of the first letter in the naming convention of restriction enzymes?

    <p>The genes</p> Signup and view all the answers

    What is the origin of the 'R' in EcoRI?

    <p>It comes from the name of the bacterial strain</p> Signup and view all the answers

    Study Notes

    Enzymes and Restriction Endonucleases

    • In 1963, two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated.
    • One of these enzymes added methyl groups to DNA, while the other cut DNA, known as a restriction endonuclease.

    Hind II Restriction Endonuclease

    • Hind II was the first restriction endonuclease to be isolated and characterized in 1968, five years after the initial discovery.
    • Hind II functions by recognizing a specific DNA nucleotide sequence and cutting DNA molecules at a particular point.
    • The specific base sequence recognized by Hind II is known as the recognition sequence.

    Restriction Enzymes

    • Over 900 restriction enzymes have been isolated from more than 230 strains of bacteria.
    • Each of these enzymes recognizes different recognition sequences.
    • The convention for naming these enzymes is based on the genes and species of the prokaryotic cell from which they were isolated.
    • Example: EcoRI comes from Escherichia coli RY 13, with the letter ‘R’ derived from the name of the strain.

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    Description

    This quiz is about the discovery of restriction enzymes in E. coli, specifically the Hind II enzyme, which cuts DNA at a specific sequence. It covers the history and function of restriction endonucleases.

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