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Questions and Answers
Quali sono le due categorie degli enzimi che tagliano il DNA?
Quali sono le due categorie degli enzimi che tagliano il DNA?
Quale è stato il primo enzima utilizzato per la restrizione del DNA?
Quale è stato il primo enzima utilizzato per la restrizione del DNA?
Quali organismi producono gli enzimi di restrizione?
Quali organismi producono gli enzimi di restrizione?
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Study Notes
- Enzymes that cut DNA are classified into two categories, class I and class II.
- Class I enzymes cut DNA near sites that are able to recognize sequences.
- Class II enzymes cut DNA within recognized sequences.
- Enzymes that restrict DNA replication are among the most commonly used enzymes.
- These enzymes are found in both bacteria and eukaryotes.
- Enzymes of restriction are endogenously produced and do not cut DNA as part of bacterial self-protection.
- Enzymes of restriction have specific three-letter codes that indicate their origin.
- The first enzyme to be used for DNA restriction was EcoRI.
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