Molecular Biology Quiz

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What is the name of the vector that allows the DNA insert to be transcribed in a host cell?

Plasmid

Cosmid

Expression vector (correct)

Adenovirus

What is the purpose of reverse transcriptase in preparing a cDNA library?

To restrict DNA

To ligate DNA fragments

To synthesize complementary DNA (correct)

To amplify DNA fragments

What is the name of the process by which a denatured nucleic acid is re-annealed with a labelled probe?

Gene transfer

PCR

Cloning

Molecular hybridization (correct)

What is the name of the animals that have an artificially introduced gene that is inherited by their offspring?

Transgenic animals Signup and view all the answers

What is the purpose of an antisense oligonucleotide?

To inhibit mRNA function Signup and view all the answers

What is the name of the type of PCR that can be used to quantitate a specific nucleic acid sequence during the reaction?

Real-time PCR Signup and view all the answers

What is the charge of proteins at the end of isoelectric focusing?

No net charge Signup and view all the answers

Which electrophoretic method is suitable for the separation of proteins according to their molecular mass?

SDS polyacrylamide gel electrophoresis Signup and view all the answers

During electrophoresis, towards which electrode do nucleic acids move?

Towards the positive electrode Signup and view all the answers

What can be concluded if the elution volume of substance 'A' is larger than that of substance 'B' during gel filtration?

The molecular mass of substance 'A' is smaller than that of substance 'B' Signup and view all the answers

What type of ligands can be used during affinity chromatography to separate specific substances?

Antibody-antigen, enzyme-substrate, hormone-receptor Signup and view all the answers

Which two separation methods fractionate particles mostly according to their molecular mass?

Gel filtration, SDS polyacrylamide gel electrophoresis Signup and view all the answers

What is the main difference between constitutive and facultative heterochromatin?

Constitutive is permanently inactive, facultative can be activated Signup and view all the answers

What are the building blocks of chromatin?

DNA, proteins, ions, and RNA Signup and view all the answers

What is the function of karyopherins?

To import or export proteins from the nucleus Signup and view all the answers

What is the correct order of chromatin organisation?

Beads-on-a-string → solenoid → looped domains → metaphase chromosome Signup and view all the answers

What type of RNA molecules can be found in chromatin?

pre-mRNA, pre-rRNA, snRNA, and snoRNA Signup and view all the answers

What is the region of chromosomes where microtubules bind during cell division?

Kinetochore Signup and view all the answers

What is the role of snoRNAs in eukaryotic cells?

Pre-rRNA processing Signup and view all the answers

Where is the maturation of ribosomal subunits finished?

Cytoplasm Signup and view all the answers

What is the name of the process that removes introns from pre-mRNA?

Splicing Signup and view all the answers

What is the role of RNA polymerase II in eukaryotic cells?

Production of pre-mRNA Signup and view all the answers

What is the name of the DNA region between the 18S and 5.8S rRNA genes?

Transcribed spacer Signup and view all the answers

What is the role of the mediator complex in eukaryotic cells?

Connecting signaling pathways to the transcriptosome Signup and view all the answers

What is the substrate of adenylate cyclase?

ATP Signup and view all the answers

Which protein kinase phosphorylates CREB?

PKA Signup and view all the answers

What is the primary effect of inositol-trisphosphate (IP3) in the cell?

Raises the cytosolic Ca++ level Signup and view all the answers