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Questions and Answers
What enzymatic activity does DNA ligase have in molecular cloning?
Why does recombinant DNA work, even though humans are very different than bacteria?
What components of the replication fork are not present in PCR, and why are they not needed?
In gel electrophoresis, in which direction does DNA run?
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What enzyme is used in PCR?
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Why does recombinant DNA work, even though humans are very different than bacteria?
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What enzymatic activity does DNA ligase have in molecular cloning?
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What components of the replication fork are not present in PCR, and why are they not needed?
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Why does recombinant DNA work, even though humans are very different than bacteria?
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What enzyme is used in PCR?
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Study Notes
Molecular Cloning
- DNA ligase has the enzymatic activity of forming a covalent bond between two DNA molecules, essential for molecular cloning.
Recombinant DNA
- Recombinant DNA works despite the differences between humans and bacteria because it relies on the universal genetic code, which allows genetic information to be translated into proteins across species.
Replication Fork and PCR
- The components of the replication fork not present in PCR are helicase, primase, and ligase, which are responsible for unwinding, priming, and sealing the DNA, respectively.
- These components are not needed in PCR because the process uses primers and DNA polymerase to amplify specific DNA sequences.
Gel Electrophoresis
- DNA runs from negative to positive electrodes in gel electrophoresis, allowing for the separation of DNA fragments based on their size and charge.
PCR
- The enzyme used in PCR is DNA polymerase, which synthesizes new DNA strands by adding nucleotides to a primer sequence.
Note: I removed duplicate questions and organized the answers into relevant topics for better clarity and understanding.
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Description
Test your knowledge of molecular biology with this quiz! From the direction of DNA in gel electrophoresis to the enzyme used in PCR and the components of the replication fork, this quiz covers a range of topics in the field. Challenge yourself and see how much you know about recombinant DNA and genetic engineering!
