Molecular Biology exam questions (2)
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Questions and Answers

Which of the following is NOT typically considered a morphological feature of a metaphase chromosome?

  • Number of chromosomes (correct)
  • Position of the centromere
  • Banding pattern
  • Chromosome length
  • What type of molecule does tRNA transport?

  • Lipids
  • Nucleotides
  • Glucose
  • Amino acids (correct)
  • In neuronal signaling, what is the primary role of glutamic acid?

  • Signaling in the peripheral nervous system exclusively.
  • Promoting neuronal firing as an excitatory neurotransmitter. (correct)
  • Inhibiting neuronal firing.
  • Directly converting into energy in neurons.
  • Which free amino acid is the precursor of hormones like thyroid hormones and catecholamines?

    <p>Tyrosine (C)</p> Signup and view all the answers

    How does tyrosine facilitate hormonal signal transmission?

    <p>By converting into hormone precursors. (B)</p> Signup and view all the answers

    In which metabolic cycle do ornithine and citrulline serve as intermediate metabolites?

    <p>Urea cycle (C)</p> Signup and view all the answers

    Which non-protein amino acid is the main inhibitory neurotransmitter in the nervous system?

    <p>γ-Aminobutyric acid (GABA) (A)</p> Signup and view all the answers

    What is produced as a by-product during the formation of a peptide bond between amino acids?

    <p>Water (A)</p> Signup and view all the answers

    How are oligosaccharide chains attached to the protein portion in glycoproteins?

    <p>Covalently, with glycosidic bonds. (B)</p> Signup and view all the answers

    Which amino acid residues are commonly phosphorylated in proteins to form phosphoproteins?

    <p>Serine and Threonine (C)</p> Signup and view all the answers

    What makes vitamins essential for bodily functions?

    <p>They act as organic coenzymes or prosthetic groups. (D)</p> Signup and view all the answers

    Which type of tRNA is responsible for initiating translation in eukaryotes?

    <p>Met-tRNAᵢMet (C)</p> Signup and view all the answers

    What is the primary function of cholesterol within the cell membrane?

    <p>Modulates membrane fluidity, maintaining flexibility and stability (D)</p> Signup and view all the answers

    What is the role of a positive control in a PCR experiment?

    <p>To verify that the analysis process is functioning properly by using a known DNA sample (D)</p> Signup and view all the answers

    What is the main purpose of cellular respiration?

    <p>To break down glucose and produce ATP (B)</p> Signup and view all the answers

    Which of the following correctly represents the sequence of events in cellular respiration?

    <p>Glycolysis → Krebs cycle → Electron transport chain (C)</p> Signup and view all the answers

    Which of the following represents the correct hierarchy of RNA structure, from simplest to most complex?

    <p>Primary, secondary, tertiary, quaternary (B)</p> Signup and view all the answers

    What effect does a long carbon chain have on the solubility of a carbohydrate in water?

    <p>Makes it less soluble in water (A)</p> Signup and view all the answers

    What term is used to describe a carbohydrate composed of more than 10 monosaccharide molecules?

    <p>Polysaccharide (C)</p> Signup and view all the answers

    The sodium-potassium pump moves ions against their concentration gradients. This process is best classified as what type of membrane transport?

    <p>Primary active transport (C)</p> Signup and view all the answers

    A secondary lysosome is formed by the fusion of which two cellular structures?

    <p>A primary lysosome with a phagosome (D)</p> Signup and view all the answers

    What term is used to describe a carbohydrate composed of up to 10 monosaccharide molecules?

    <p>Oligosaccharide (A)</p> Signup and view all the answers

    What key event occurs when an mRNA molecule binds to a ribosome during translation?

    <p>The large subunit and small subunit become conjoined to begin translation (C)</p> Signup and view all the answers

    What type of proteins are produced by ribosomes that freely float in the cytoplasm?

    <p>Proteins that function in the cytosol (B)</p> Signup and view all the answers

    What type of substances are primarily stored within amyloplasts?

    <p>Carbohydrates in the form of starch grains (A)</p> Signup and view all the answers

    Which cellular function is primarily carried out by lysosomes?

    <p>Break down of carbohydrates, proteins, fats and nucleic acids (C)</p> Signup and view all the answers

    How would you characterize heterochromatin during interphase?

    <p>All answers are correct (D)</p> Signup and view all the answers

    What is the first amino acid in all proteins produced by the mitochondria, chloroplasts, and bacteria?

    <p>N-formylmethionine (B)</p> Signup and view all the answers

    What is the likely outcome if the annealing temperature is set too high in a PCR reaction?

    <p>The primers will not bind to the template. (A)</p> Signup and view all the answers

    What type of ligands are "orphan" receptors intended, concerning cell signaling?

    <p>Unknown ligands (A)</p> Signup and view all the answers

    What describes lipid rafts in cell membranes?

    <p>Lipid-rich regions in cell membranes involved in signaling and trafficking (B)</p> Signup and view all the answers

    Where is the electron transport chain located in eukaryotic cells?

    <p>The inner membrane of the mitochondria (A)</p> Signup and view all the answers

    Which of the following is the most accurate description of the main components of the cytoplasm?

    <p>Water, salts, and organic molecules (D)</p> Signup and view all the answers

    At which structural level is a protein said to be when it contains disulfide bridges?

    <p>Tertiary structure (B)</p> Signup and view all the answers

    Which of the following correctly lists types of chromosomes based on centromere position?

    <p>Metacentric, submetacentric, acrocentric, and telocentric chromosomes (A)</p> Signup and view all the answers

    The formation of a phagosome is directly associated with which type of membrane transport?

    <p>Phagocytosis (C)</p> Signup and view all the answers

    Which of the nitrogenous bases listed below is a purine, characterized by its double-ring structure?

    <p>Adenine (A)</p> Signup and view all the answers

    During the transition from interphase to metaphase, by approximately how much does DNA shorten to form chromosomes?

    <p>10,000x (C)</p> Signup and view all the answers

    What distinguishes autosomes from allosomes in a cell?

    <p>Autosomes are non-sex chromosomes, and allosomes are sex chromosomes (D)</p> Signup and view all the answers

    What role do cristae play within mitochondria?

    <p>To increase surface area for ATP production (B)</p> Signup and view all the answers

    Which of the nitrogenous bases listed below is pyrimidine, characterized by a single-ring structure?

    <p>Cytosine (C)</p> Signup and view all the answers

    Which compound is essential for efficient aerobic respiration, enabling cells to break down macromolecules for energy in the form of ATP?

    <p>Oxygen (D)</p> Signup and view all the answers

    In DNA condensation, which histone protein is directly involved in the formation of the 30nm fiber?

    <p>formation of the 30nm fiber (C)</p> Signup and view all the answers

    What is the primary function of centrosomes in a cell?

    <p>To organize microtubules and form the mitotic spindle (B)</p> Signup and view all the answers

    During cell division, what is the kinetochore's main function?

    <p>To attach chromosomes to the spindle during cell division (D)</p> Signup and view all the answers

    Which type of ligands are typically transported through the circulatory system to reach distant target cells?

    <p>Only hormones, like adrenalin (epinephrine) (B)</p> Signup and view all the answers

    What percentage of a typical cell's composition is water?

    <p>80-90% (D)</p> Signup and view all the answers

    Which of the following phospholipid movements within the cell membrane requires enzymatic activity?

    <p>Transverse “flip-flop” movement (B)</p> Signup and view all the answers

    What is a codon?

    <p>Three nucleotides of RNA (D)</p> Signup and view all the answers

    In PCR, what does a positive control accomplish?

    <p>To ensure that the PCR process works by using a known DNA sample (A)</p> Signup and view all the answers

    What defines stem cells?

    <p>Cells that have the ability to divide and differentiate into various cell types (A)</p> Signup and view all the answers

    How would you describe the interphase of the cell cycle?

    <p>The phase when the cell is preparing for division (A)</p> Signup and view all the answers

    Which interaction is NOT directly involved in the formation of the tertiary structure of RNA?

    <p>Creation of an A-type helix (D)</p> Signup and view all the answers

    Which of the following is a key characteristic of the pseudouridine loop, also known as the T-loop, of tRNA?

    <p>It contains the site where the tRNA binds to the ribosome (D)</p> Signup and view all the answers

    What does cell signaling achieve in the context of junxacrina?

    <p>A mechanism by which cells respond to external signals to regulate growth (C)</p> Signup and view all the answers

    Which type of receptor is typically designed for neurotransmitters when a rapid cellular response is required?

    <p>Ionotropic (ion-channel-coupled) receptors (B)</p> Signup and view all the answers

    What primarily determines the progression of the cell cycle?

    <p>Cyclin-dependent protein kinases (cdk), which become enzymatically active due to the attachment of cyclins (C)</p> Signup and view all the answers

    What are mitogens?

    <p>Extracellular signal molecules that stimulate cell proliferation (A)</p> Signup and view all the answers

    What specific process drives the elongation phase of DNA replication towards irreversibility?

    <p>Hydrolysis of pyrophosphate (PPi) to inorganic phosphate Pi (A)</p> Signup and view all the answers

    Following 6 PCR cycles, how many copies of the original DNA sequence are theoretically produced?

    <p>64 copies (D)</p> Signup and view all the answers

    What is the mechanism of action known as combinatorial control of gene expression?

    <p>This is a process that involves the use of a group of transcriptional regulators working together to express a gene correctly (B)</p> Signup and view all the answers

    Which of the following accurately describes primary active transport?

    <p>Transport of molecules from low to high concentration using energy from ATP (B)</p> Signup and view all the answers

    What best describes the function or role of Mitogen-Activated Protein Kinase (MAPK) in cellular signaling pathways?

    <p>The final serine-threonine kinase following the direct activation of Ras protein (C)</p> Signup and view all the answers

    In addition to a protein component, what other type of molecule is characteristically found in a glycoprotein?

    <p>Oligosaccharides (C)</p> Signup and view all the answers

    How are carbohydrates composed of more than ten subunits best classified?

    <p>Polysaccharides (C)</p> Signup and view all the answers

    Which method describes how hematopoietic stem cells (HSCs) are typically collected via apheresis?

    <p>Using fluorescent antibodies selective for the CD34 surface protein (C)</p> Signup and view all the answers

    What type of chemical bond is primarily responsible for the interaction between codon and anticodon bases during translation?

    <p>Hydrogen bonds (A)</p> Signup and view all the answers

    Within a lipid bilayer, where are the hydrophobic components of the lipids primarily located?

    <p>Inside the lipid bilayer (D)</p> Signup and view all the answers

    Which DNA repair pathway utilizes the MGMT protein to directly remove the methyl group from the O6 position of guanine?

    <p>Direct repair (DR) (A)</p> Signup and view all the answers

    Which histone protein is not a component of the nucleosome core particle?

    <p>H1 (A)</p> Signup and view all the answers

    During which phase of the cell cycle is the concentration of mitotic cyclin (cyclin B) typically at its highest level?

    <p>In phase M (B)</p> Signup and view all the answers

    Under what circumstances does programmed cell death, also known as apoptosis, typically occur in multicellular organisms?

    <p>When the activation of a cell-death intracellular program is initiated (D)</p> Signup and view all the answers

    During what process does the number of chromosomes of a cell become most visually distinguishable using light microscopy?

    <p>Metaphase (A)</p> Signup and view all the answers

    Which of the following molecules does tRNA primarily transport to the ribosome during protein synthesis?

    <p>Amino acids (B)</p> Signup and view all the answers

    What role does the enzyme glutamine synthetase play in neuronal signaling pathways?

    <p>Removal of glutamate from the synaptic cleft (D)</p> Signup and view all the answers

    Which amino acid serves as a direct precursor in the biosynthesis of melanin, the pigment responsible for skin and hair color?

    <p>Tyrosine (B)</p> Signup and view all the answers

    How does tyrosine kinase activity primarily contribute to hormonal signal transduction?

    <p>By phosphorylating intracellular proteins to initiate signaling cascades (D)</p> Signup and view all the answers

    Which amino acids are classified as acidic due to their side chain properties?

    <p>Glutamic acid and aspartic acid (C)</p> Signup and view all the answers

    In what metabolic process do ornithine and citrulline function as key intermediate metabolites?

    <p>Urea cycle (A)</p> Signup and view all the answers

    Which non-protein amino acid primarily functions as the main inhibitory neurotransmitter in the nervous system?

    <p>γ-Aminobutyric acid (GABA) (A)</p> Signup and view all the answers

    What molecule results as a by-product during the formation of a peptide bond between amino acids?

    <p>Water (C)</p> Signup and view all the answers

    How are oligosaccharide chains covalently attached to proteins during the synthesis of glycoproteins?

    <p>Covalently, through glycosidic bonds that link the oligosaccharides to specific amino acid residues. (B)</p> Signup and view all the answers

    Which amino acid residues are commonly phosphorylated in proteins to regulate their function?

    <p>Serine and Threonine (C)</p> Signup and view all the answers

    Why are vitamins essential for the human body?

    <p>They serve as organic coenzymes or prosthetic groups that facilitate key biochemical reactions. (A)</p> Signup and view all the answers

    Which checkpoint in the cell cycle ensures proper attachment of chromosomes to the spindle fibers before the initiation of anaphase?

    <p>M checkpoint (B)</p> Signup and view all the answers

    Which characteristic accurately describes the pseudouridine loop, also known as the T-loop, of tRNA?

    <p>It contains the site where the tRNA binds to the ribosome (B)</p> Signup and view all the answers

    Which type of receptors are specifically designed for neurotransmitters when a rapid cellular response is necessary?

    <p>Ionotropic (ion-channel-coupled) receptors (B)</p> Signup and view all the answers

    Which process drives the elongation phase of DNA replication towards irreversibility?

    <p>Hydrolysis of pyrophosphate (PPi) to inorganic phosphate (Pi) (D)</p> Signup and view all the answers

    What is combinatorial control of gene expression?

    <p>A process that involves the use of a group of transcriptional regulators working together to express a gene correctly. (A)</p> Signup and view all the answers

    What kind of bonds form between the codon-anticodon bases during translation?

    <p>Hydrogen bonds (B)</p> Signup and view all the answers

    What DNA repair process involves the MGMT protein, which removes the oxygen-bound methyl group O6 of guanine?

    <p>Direct repair (DR) (A)</p> Signup and view all the answers

    Which histone protein does NOT form part of the nucleosome core?

    <p>H1 (B)</p> Signup and view all the answers

    How does cholesterol affect the cell membrane?

    <p>Modulates membrane fluidity, maintaining flexibility and stability (A)</p> Signup and view all the answers

    In which phase of the cell cycle is the concentration of mitotic cyclin (cyclin B) the highest?

    <p>In phase M (C)</p> Signup and view all the answers

    Under what conditions does programmed cell death (apoptosis) typically occur in multicellular organisms?

    <p>When the activation of a cell-death intracellular program is initiated (B)</p> Signup and view all the answers

    Which sequence of events accurately describes cellular respiration?

    <p>Glycolysis → Krebs cycle → Electron transport chain (B)</p> Signup and view all the answers

    What effect does a long carbon chain have on a carbohydrate's solubility in water?

    <p>Makes it less soluble in water (D)</p> Signup and view all the answers

    What term describes a carbohydrate containing more than 10 monosaccharide molecules?

    <p>Polysaccharide (D)</p> Signup and view all the answers

    What term describes a carbohydrate containing up to 10 monosaccharide molecules?

    <p>Oligosaccharide (D)</p> Signup and view all the answers

    What type of proteins are produced by ribosomes freely floating in the cytoplasm?

    <p>Proteins that function in the cytosol (A)</p> Signup and view all the answers

    What substances are primarily stored within amyloplasts?

    <p>Carbohydrates in the form of starch grains (C)</p> Signup and view all the answers

    Which of the following accurately describes the main components of the cytoplasm?

    <p>Water, salts, and organic molecules (A)</p> Signup and view all the answers

    What level of protein structure is indicated by the presence of disulfide bridges?

    <p>Tertiary structure (B)</p> Signup and view all the answers

    Which nitrogenous base is a purine characterized by its double-ring structure?

    <p>Adenine (B)</p> Signup and view all the answers

    Which of the following nitrogenous bases is classified as a pyrimidine due to its single-ring structure?

    <p>Cytosine (C)</p> Signup and view all the answers

    Which of the following best describes lipid rafts in cell membranes?

    <p>Lipid-rich regions in cell membranes involved in signaling and trafficking (A)</p> Signup and view all the answers

    Which of the following are types of chromosomes?

    <p>Metacentric, submetacentric, acrocentric, and telocentric chromosomes (C)</p> Signup and view all the answers

    What is the difference between autosomes and allosomes?

    <p>Autosomes are non-sex chromosomes, and allosomes are sex chromosomes (A)</p> Signup and view all the answers

    What is the function of the cristae in mitochondria?

    <p>To increase surface area for ATP production (A)</p> Signup and view all the answers

    What is the function of centrosomes in the cell?

    <p>To organize microtubules and form the mitotic spindle (C)</p> Signup and view all the answers

    What is the function of the kinetochore?

    <p>To attach chromosomes to the spindle during cell division (B)</p> Signup and view all the answers

    What percentage of a cell is water?

    <p>80-90% (B)</p> Signup and view all the answers

    What is the purpose of a positive control in PCR?

    <p>To ensure that the PCR process works by using a known DNA sample (D)</p> Signup and view all the answers

    What are stem cells?

    <p>Cells that have the ability to divide and differentiate into various cell types (C)</p> Signup and view all the answers

    What phase of the cell cycle is interphase?

    <p>The phase when the cell is preparing for division (C)</p> Signup and view all the answers

    What is cell signaling in the context of junxacrine?

    <p>A process where cells communicate through physical contact (C)</p> Signup and view all the answers

    The course of the cell cycle is primarily determined by?

    <p>Cyclin-dependent protein kinases (cdk), which become enzymatically active due to the attachment of cyclins (C)</p> Signup and view all the answers

    How many copies of DNA are produced after 6 PCR cycles?

    <p>64 copies (C)</p> Signup and view all the answers

    Which of the following best describes primary active transport?

    <p>Transport of molecules from low to high concentration using energy from ATP (C)</p> Signup and view all the answers

    Which of the following represents the highest level of structural organization in RNA?

    <p>Quaternary structure of RNA (C)</p> Signup and view all the answers

    What functions do lysosomes perform in the cell?

    <p>Break down of carbohydrates, proteins, fats and nucleic acids (D)</p> Signup and view all the answers

    Which of the following phospholipid movements may be catalyzed by enzymes in the cell membrane?

    <p>Transverse “flip-flop” movement (B)</p> Signup and view all the answers

    How is the START codon recognized in eukaryotes during translation initiation?

    <p>By scanning along the mRNA from the 5' end (A)</p> Signup and view all the answers

    Which of the following is a component of a glycolipid?

    <p>sphingosine (A)</p> Signup and view all the answers

    Which of the following DNA strands can form a DNA duplex by pairing with itself at each position?

    <p>5’-AAGCCGTT-3’ (C)</p> Signup and view all the answers

    What is the role of EDTA in blood collection and laboratory experiments?

    <p>Prevents blood clotting by binding calcium ions (C)</p> Signup and view all the answers

    What is a key safety consideration when using Midori Green in molecular biology experiments?

    <p>It is toxic and should be handled with care, using gloves and eye protection (C)</p> Signup and view all the answers

    What is the primary use of Midori Green in molecular biology?

    <p>Stains the DNA for visualization in gel electrophoresis (D)</p> Signup and view all the answers

    What is the structure of a DNA helix?

    <p>A double-stranded, right-handed helix (D)</p> Signup and view all the answers

    In PCR, what is the purpose of the denaturation step?

    <p>To separate the DNA strands by breaking hydrogen bonds (B)</p> Signup and view all the answers

    What is the function of mitochondria in human cells?

    <p>To generate ATP through cellular respiration (B)</p> Signup and view all the answers

    What is the typical annealing temperature used in PCR?

    <p>72°C (C)</p> Signup and view all the answers

    What is the main feature of the structure of biological membranes?

    <p>A fluid bilayer of phospholipids with embedded proteins (B)</p> Signup and view all the answers

    What is deoxyribonucleic acid (DNA) made of?

    <p>Nucleotides containing a phosphate group, sugar, and nitrogenous base (A)</p> Signup and view all the answers

    Agarose is made of which of the following?

    <p>Polysaccharide from seaweed (A)</p> Signup and view all the answers

    Flashcards

    Metaphase Chromosome Features

    Characteristics including banding pattern, centromere position, number, and length.

    Banding Pattern

    The specific pattern of light and dark bands on a chromosome during metaphase.

    Centromere Position

    The location of the centromere on the chromosome, affecting its behavior during cell division.

    Number of Chromosomes

    The total count of chromosomes in a cell, defining species-specific traits.

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    tRNA Function

    tRNA transports amino acids to the ribosome for protein synthesis.

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    Glutamic acid

    A primary excitatory neurotransmitter that promotes neuronal firing.

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    Tyrosine

    A free amino acid precursor for hormones like thyroid hormones and catecholamines.

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    Hormonal signal transmission

    Tyrosine is converted into precursors for hormones, facilitating signal transmission.

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    Urea cycle

    The metabolic cycle where ornithine and citrulline serve as important intermediates.

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    GABA

    Main inhibitory neurotransmitter in the nervous system, derived from glutamate.

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    Peptide bond formation

    A condensation reaction that produces water as a by-product when forming a peptide bond.

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    Glycoprotein attachment

    Oligosaccharide chains are attached to proteins via glycosidic bonds to specific amino acids.

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    Phosphoproteins

    Commonly phosphorylated amino acid residues in proteins include serine and threonine.

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    Vitamins

    Essential for body functions because they serve as coenzymes or prosthetic groups in biochemical reactions.

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    M checkpoint

    The checkpoint ensuring all chromosomes are properly attached to spindle fibers during mitosis.

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    Peptides

    Short chains of amino acids linked by peptide bonds.

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    Codons

    Three-nucleotide sequences in mRNA that specify amino acids.

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    Tertiary structure of RNA

    The 3D shape of RNA, formed by various interactions.

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    Pseudouridine loop (T-loop)

    Region in tRNA crucial for ribosome binding, contains unpaired CCA sequence.

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    Ionotropic receptors

    Receptors that respond quickly to neurotransmitters by opening ion channels.

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    PPi in DNA replication

    The release of pyrophosphate that makes DNA replication irreversible.

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    Combinatorial control

    Gene expression regulated by multiple transcriptional regulators together.

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    MAPK

    A key kinase involved in signal transduction after Ras activation.

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    Glycoprotein components

    Proteins that can include oligosaccharides as components.

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    Polysaccharides

    Carbohydrates made of more than ten sugar units.

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    Codon-anticodon interactions

    Base pairs connected by hydrogen bonds during translation.

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    Nucleotide excision repair

    DNA repair mechanism that removes damaged bases and synthesizes new DNA.

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    Metacentric chromosomes

    Chromosomes with arms of equal length.

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    Anaphase of mitosis

    Phase where sister chromatids separate to opposite poles.

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    Cell as the smallest living unit

    The basic unit of life that can carry out all functions needed for life.

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    Sodium-potassium pump

    A type of primary active transport that moves sodium out and potassium into the cell against their concentration gradients.

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    Secondary lysosome

    Formed by the fusion of a primary lysosome with a phagosome, allowing for the breakdown of larger particles.

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    mRNA and ribosome interaction

    When mRNA attaches to a ribosome, the large and small subunits come together to initiate translation.

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    Lysosome functions

    Breakdown of carbohydrates, proteins, fats, and nucleic acids within the cell.

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    Heterochromatin in interphase

    Genetically inactive chromatin that is dark-staining and condensed.

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    Orphan receptors

    Receptors intended for unknown ligands that have not yet been identified.

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    Electron transport chain location

    Occurs in the inner membrane of the mitochondria, generating ATP.

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    Phagosome formation

    Involves membrane transport associated with phagocytosis, where cells engulf large particles.

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    DNA shortening in metaphase

    DNA condenses into chromosomes that are 10,000x shorter during metaphase.

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    Aerobic respiration

    A process that requires oxygen to break down macromolecules for ATP energy.

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    Chromatin formation

    Histone H1 is involved in the formation of the 30 nm fiber structure of chromatin.

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    Biological membranes structure

    A fluid bilayer of phospholipids with embedded proteins, allowing selective permeability.

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    PCR denaturation step

    To separate DNA strands by breaking hydrogen bonds for replication.

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    Role of mitochondria

    Generate ATP through cellular respiration, acting as the energy currency of the cell.

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    Lipid Rafts

    Lipid-rich regions in cell membranes involved in signaling and trafficking.

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    Types of Chromosomes

    Metacentric, submetacentric, acrocentric, and telocentric chromosomes.

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    Autosomes vs Allosomes

    Autosomes are non-sex chromosomes, while allosomes are sex chromosomes.

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    Cristae Function

    Cristae increase surface area for ATP production in mitochondria.

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    Centrosome Function

    Centrosomes organize microtubules and form the mitotic spindle.

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    Kinetochore

    Kinetochore attaches chromosomes to the spindle during cell division.

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    Cell Water Percentage

    Approximately 70% of a cell is water.

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    Positive Control in PCR

    A known DNA sample used to ensure the PCR process works.

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    Stem Cells

    Cells that can divide and differentiate into various cell types.

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    Interphase

    Interphase is the phase when the cell is preparing for division.

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    Cell Cycle Determination

    Primarily determined by cyclin-dependent protein kinases (cdk).

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    Mitogens

    Extracellular signal molecules that stimulate cell proliferation.

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    Primary Active Transport

    Transport of molecules from low to high concentration using energy from ATP.

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    Totipotent Stem Cells

    Stem cells that can form all cell types, including placental tissues.

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    Structure of DNA

    A polymer of nucleotides in a double helix form.

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    Cellular Respiration Purpose

    To break down glucose and produce ATP for energy.

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    Events in Cellular Respiration

    The correct sequence is Glycolysis → Krebs cycle → Electron transport chain.

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    Long Carbon Chain in Carbohydrates

    Makes a carbohydrate less soluble in water.

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    Oligosaccharide

    A carbohydrate composed of up to 10 monosaccharides.

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    Function of Cytoplasmic Ribosomes

    Produce proteins that function in the cytosol.

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    Amyloplasts

    Cells that primarily contain starch grains.

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    First Amino Acid in Proteins

    The first amino acid in mitochondrial proteins is Methionine.

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    PCR Annealing Temperature

    If set too high, primers will not bind to the template.

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    Degeneracy of Genetic Code

    An amino acid can be encoded by more than one codon.

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    Amino Acid with Thiol Group

    Cysteine contains a thiol (-SH) group in its side chain.

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    Excitatory Neurotransmitters

    Glutamic acid and aspartic acid primarily act as excitatory neurotransmitters.

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    Role of Serine and Glycine

    Serve as precursors in the synthesis of phospholipids.

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    Cyclic Non-Polar Amino Acid

    Proline is a non-polar amino acid with a cyclic side chain.

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    Non-morphological feature of Chromosome

    The feature that is not related to chromosome structure during metaphase, specifically counting chromosomes.

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    Quaternary structure of RNA

    The arrangement of multiple RNA strands into a complex.

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    Heterochromatin

    Genetically inactive chromatin that appears dark-staining and condensed during interphase.

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    Aerobic Respiration Component

    Oxygen is necessary for aerobic respiration to break down macromolecules for ATP.

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    DNA Duplex Formation

    A strand of DNA that can form a double helix by pairing with itself.

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    PCR Denaturation Step Purpose

    To separate DNA strands by breaking hydrogen bonds for replication.

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    Structure of DNA Helix

    A double-stranded, right-handed helical structure composed of nucleotides.

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    Start Codon Recognition

    In eukaryotes, recognized by the initiator tRNA binding to the ribosome with mRNA.

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    Glycolipid Component

    Sphingosine is a key component of a glycolipid structure.

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    Glutamic Acid Function

    It is a primary excitatory neurotransmitter that promotes neuronal firing.

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    Tyrosine Hormone Precursor

    Tyrosine is a free amino acid precursor for hormones like thyroid hormones and catecholamines.

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    Tyrosine's Role in Hormones

    Tyrosine is converted into precursors for hormones, facilitating hormonal signal transmission.

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    Ornithine and Citrulline

    These non-protein amino acids are important intermediates in the urea cycle.

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    Peptide Bond By-product

    The by-product of the peptide bond formation is water.

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    Phosphorylated Amino Acids

    Serine and threonine are the most commonly phosphorylated amino acid residues in proteins.

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    Vitamins' Essential Role

    Vitamins serve as organic coenzymes or prosthetic groups that facilitate biochemical reactions.

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    Mitosis Checkpoint

    The M checkpoint ensures that all chromosomes are attached to spindle fibers before separation during mitosis.

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    DNA replication irreversible process

    The addition of nucleotides makes DNA replication irreversible, due to PPi release.

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    Combinatorial control of gene expression

    Gene expression regulated by a group of transcriptional regulators working together.

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    Mitogen-activated Protein Kinase (MAPK)

    A key kinase activated in a signaling pathway after Ras activation. It phosphorylates proteins.

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    Polysaccharides definition

    Carbohydrates consisting of more than ten monosaccharide units.

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    Mitosis phase with chromosome separation

    Anaphase is where sister chromatids separate to opposite poles of the cell.

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    Cellular apoptosis triggers

    Programmed cell death initiated by activating a cell-death program.

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    Histone outside nucleosome core

    Histone H1 does not form part of the nucleosome core but plays a stabilizing role.

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    Hydrophobic components of lipid bilayer

    The lipid bilayer's hydrophobic components are located inside the membrane.

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    Cyclin B concentration in cell cycle

    Cyclin B concentration is highest in the M phase (mitosis) of the cell cycle.

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    Sequence of Events in Cellular Respiration

    The correct order is Glycolysis → Krebs cycle → Electron transport chain.

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    Long Carbon Chain Effect in Carbohydrates

    Makes a carbohydrate less soluble in water.

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    Nitrogenous Bases Structures

    Cytosine is a single-ring (pyrimidine) structure, while Adenine is a double-ring (purine).

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    Study Notes

    Morphological Features of Metaphase Chromosomes

    • Banding pattern, position of the centromere, and chromosome length are morphological features.
    • Number of chromosomes is not a morphological feature; it's a numerical characteristic.

    tRNA Transport

    • tRNA transports amino acids, not peptides, codons, or proteins.

    RNA Tertiary Structure Interactions

    • Not all interactions are involved: Formation of an A-type helix, interactions of arm stems, and binding of basic proteins/ions are involved.
    • Formation of non-canonical pairs/triplets is also a critical interaction.
    • Creation of an A-type helix, Interactions of arm stems with other arm stems, Binding of basic proteins or ions that neutralize the negative charge of RNA are involved in the formation of a tertiary structure of RNA. The exclusionary statement is the formation of non-canonical pairs/triplets.

    Pseudouridine Loop (T-loop) of tRNA

    • The T-loop contains an unpaired CCA sequence at the 3' end. It does not contain a D-loop, mobilize the tRNA directly, or contain the ribosome binding site.

    Receptors for Neurotransmitters

    • Ionotropic (ion-channel-coupled) receptors are designed for rapid responses to neurotransmitters.

    DNA Replication Irreversibility

    • Hydrolysis of pyrophosphate (PPi) to inorganic phosphate (Pi) during DNA replication makes the process irreversible.

    Combinatorial Control of Gene Expression

    • Combinatorial control involves the coordinated action of multiple transcriptional regulators for proper gene expression.

    Mitogen-Activated Protein Kinase (MAPK)

    • MAPK is a serine-threonine kinase that plays a crucial role in cellular signaling pathways, receiving the final phosphorylation signal from Ras activation.
    • MAPK is the final serine-threonine kinase following the activation of Ras protein.

    Glycoprotein Components

    • Oligosaccharides are a component of glycoproteins, in addition to the protein itself. Lipids and metal ions aren't components of glycoproteins.

    Carbohydrate Subunit Classification

    • Carbohydrates with more than 10 subunits are called polysaccharides.

    HSC Collection via Apheresis

    • HSCs are collected using fluorescent antibodies that specifically target the CD34 surface protein.

    Codon-Anticodon Bonds

    • Hydrogen bonds form between codon-anticodon bases.

    Lipid Bilayer Hydrophobic Components

    • Hydrophobic components are located inside the lipid bilayer.

    DNA Repair via MGMT

    • Direct repair (DR) involving the MGMT protein removes the oxygen-bound methyl group from O6 of guanine.

    Histone Not in Nucleosome Core

    • Histone H1 is not part of the nucleosome core.

    Mitotic Cyclin Concentration

    • The concentration of mitotic cyclin (cyclin B) is highest in the M phase of the cell cycle.

    Programmed Cell Death (Apoptosis)

    • Apoptosis occurs when a cell-death intracellular program is initiated, and it's not limited to embryonic development or requiring energy.

    Amino Acids in Human Proteins

    • There are 20 basic amino acids found in human proteins.

    Protein Secondary Structure

    • The hydrogen bond between the NH and CO of amino acid residues i and i+3 stabilizes the α-helix.

    Interphase Phases

    • Interphase includes the G1, S, and G2 phases.

    Nucleotide Excision Repair

    • Nucleotide excision repair involves XPG and XPF endonucleases cutting DNA strands around the damage, helicases unwinding the DNA, the damaged DNA being removed, and DNA polymerase and ligase synthesizing and sealing the new DNA strand.
    • Step that does NOT occur in nucleotide excision repair is the "Removal of the damaged nitrogenous base by specific DNA glycosylase".

    Chromosome Arm Length

    • A metacentric chromosome has arms of equal length.

    Smallest Living Unit

    • A cell is the smallest living unit.

    Chromosome Position Between Divisions

    • Chromosomes occupy distinct territories in the interphase nucleus, not the nucleolus.

    RNA Structure with Apical Loops

    • Apical loops (hairpens) exist in the secondary RNA structure.

    Sodium-Potassium Pump

    • The sodium-potassium pump is categorized as primary active transport.

    Secondary Lysosome Formation

    • A secondary lysosome is the result of the fusion of a primary lysosome with a phagosome.

    mRNA and Ribosomes

    • When mRNA attaches to a ribosome, the large and small subunits join and begin translation, not separating or changing sedimentation rate.

    Lysosome Function

    • Lysosomes break down carbohydrates, proteins, fats, and nucleic acids.

    Heterochromatin in Interphase

    • Heterochromatin is genetically inactive, stains dark under the microscope, and is in a condensed state during interphase.

    Orphan Receptors and Ligands

    • "Orphan" receptors are intended for unknown ligands.

    Electron Transport Chain Location

    • The electron transport chain occurs in the inner membrane of the mitochondria.

    Membrane Transport and Phagosome Formation

    • Phagosome formation utilizes phagocytosis, which is a type of endocytosis.

    Interphase DNA Shortening

    • Interphase DNA condenses approximately 10,000 times to form metaphase chromosomes.

    Aerobic Respiration and Compound

    • Oxygen is necessary for aerobic respiration.

    DNA Condensation with Histone H1

    • Histone H1 is involved in the formation of the 30nm fiber during DNA condensation.

    Circulatory Transport Ligands

    • Hormones and neurotransmitters are transported through the circulatory system.

    Phospholipid Movement

    • Lateral diffusion and rotational motion of phospholipids in cell membranes are catalyzed by enzymes; not transverse “flip-flop” movement.

    Codon Definition

    • A codon is three nucleotides of RNA.

    START Codon Recognition in Eukaryotes

    • The START codon is recognized through the binding of the small ribosomal subunit, mRNA, and initiator tRNA. This involves scanning from the 5' end.

    Glycolipid Component

    • Sphingosine is a component of glycolipids.

    DNA Sequence for Duplex Formation

    • 5’-AAGCCGTT-3’ can form a DNA duplex with itself.

    EDTA Role in Blood Analysis

    • EDTA is an anticoagulant that prevents blood clotting by binding calcium ions.

    Midori Green Safety

    • Midori Green is toxic and requires safety precautions like gloves and eye protection.

    Midori Green Use

    • Midori Green stains DNA for visualization during gel electrophoresis.

    DNA Helix Structure

    • DNA forms a double-stranded, right-handed helix.

    PCR Denaturation Step

    • The denaturation step in PCR separates the DNA strands by breaking hydrogen bonds.

    Mitochondria Function

    • Mitochondria generate ATP through cellular respiration.

    PCR Annealing Temperature

    • The annealing temperature typically ranges from 50-65°C in PCR.

    Biological Membrane Structure

    • Biological membranes consist of a fluid bilayer of phospholipids with embedded proteins.

    DNA Components

    • DNA is composed of nucleotides with a phosphate group, sugar, and nitrogenous base.

    Agarose Composition

    • Agarose is a polysaccharide derived from seaweed.

    Lipid Rafts

    • Lipid rafts are lipid-rich regions in cell membranes involved in signaling and trafficking.

    Chromosome Types

    • Chromosomes can be prokaryotic or eukaryotic; types include metacentric, submetacentric, acrocentric, and telocentric.

    Autosomes vs. Allosomes

    • Autosomes are non-sex chromosomes, and allosomes are sex chromosomes.

    Mitochondria Cristae Function

    • Cristae increase the surface area of the mitochondria, which is important for ATP production.

    Centrosome Function

    • Centrosomes organize microtubules and form the mitotic spindle.

    Kinetochore Function

    • Kinetochores attach chromosomes to the spindle during cell division.

    Cell Water Content

    • Cells are typically 50-70% water.

    PCR Positive Control Role

    • A positive control in PCR ensures the process is working by using a known sample.

    Stem Cells

    • Stem cells are cells that can divide and differentiate into various cell types.

    Interphase Cell Cycle Phase

    • Interphase is the phase of the cell cycle where the cell is preparing for division, growing, or completing other essential activities. Not actively dividing or completing mitosis.

    Cell Signaling

    • Cell signaling, in the context of junxacrina, is the process by which cells respond to external signals to regulate growth and other processes; not directly about junxacrina protein synthesis.

    Cell Cycle Regulation

    • The progression through the cell cycle is primarily controlled by cyclin-dependent protein kinases (CDKs), with cyclins needed for proper enzymatic activity.

    Mitogens

    • Mitogens are extracellular signal molecules that stimulate cell proliferation.

    PCR Cycle DNA Copies

    • After 6 PCR cycles, there are 64 copies of DNA.

    Primary Active Transport

    • Primary active transport moves molecules from low to high concentration using energy from ATP.

    Totipotent Stem Cells

    • Totipotent stem cells can form all cell types in the body, including extra-embryonic tissues.

    Protein Kinase C (PKC)

    • PKC adds phosphate groups to proteins, regulating cellular processes.

    DNA Structure

    • DNA is a double-stranded molecule composed of nucleotides, with each nucleotide containing a nitrogenous base, a five-carbon sugar, and a phosphate group.

    Amino Acid Core Structure

    • The core structure of an amino acid has a central carbon atom bonded to an amino group, carboxyl group, and a hydrogen atom (and a side chain that varies).

    Amino Acid Backbone

    • The backbone of an amino acid, neglecting hydrogen atoms, is C-C-N.

    Number of Amino Acids

    • There are 20 standard amino acids found in natural proteins.

    RNA Concentration in a Cell

    • RNA concentration in a typical cell is estimated at 1-5% or 30-40%.

    Dominant RNA Type

    • Ribosomal RNA (rRNA) is the most dominant type of RNA in a cell.

    Potassium and Sodium Ion Concentration

    • Potassium (K⁺) ions are most concentrated intracellularly, and sodium (Na⁺) ions are most concentrated extracellularly.

    Cholesterol in Cell Membranes

    • Cholesterol modulates membrane fluidity, helping to maintain flexibility and stability.

    PCR Positive Control Function

    • A positive control in PCR verifies the analysis process is functioning by using a known sample.

    Cellular Respiration Purpose

    • Cellular respiration breaks down glucose to produce ATP.

    Cellular Respiration Sequence

    • Cellular respiration follows the sequence: Glycolysis → Krebs cycle → Electron transport chain.

    Carbohydrate Chain Length and Solubility

    • Longer carbon chains in carbohydrates make them less soluble in water.

    Carbohydrate Classification by Subunits

    • Carbohydrates with more than 10 subunits are called polysaccharides; those with up to 10 are oligosaccharides.

    Cytoplasm Ribosome Function

    • Ribosomes floating freely in the cytoplasm produce proteins that function within the cell's cytosol.

    Amyloplast Contents

    • Amyloplasts primarily contain starch grains.

    First Amino Acid in Proteins

    • The first amino acid in proteins produced by mitochondria, chloroplasts, and bacteria is N-formylmethionine.

    PCR Annealing Temperature Impacts

    • Setting the annealing temperature too high in PCR prevents primers from binding to the template DNA.

    Cytoplasm Components

    • The cytoplasm is primarily composed of water, salts, and organic molecules.

    Disulfide Bridges in Protein Structure

    • Proteins with disulfide bridges primarily exhibit tertiary structure.

    Nitrogenous Base Types

    • Adenine and guanine are double-ring (purine) bases; cytosine, thymine, and uracil (in RNA) are single-ring (pyrimidine) bases.

    Genetic Code Degeneracy

    • Degeneracy of the genetic code means that multiple codons can specify the same amino acid, mostly differing at the third nucleotide position.

    Thiol-Containing Amino Acid

    • Cysteine contains a thiol (-SH) group.

    Hydroxyl-Containing Amino Acid

    • Threonine contains a hydroxyl (-OH) group.

    Nonpolar Aliphatic Amino Acid

    • Leucine is a nonpolar, aliphatic amino acid.

    Cyclic Side Chain Amino Acid

    • Proline has a cyclic side chain.

    α-Amino Acid Classification

    • α-amino acids (except glycine) are classified as D or L based on the orientation of the amino group relative to the chiral carbon in a Fischer projection.

    L-Amino Acid Designation

    • An amino acid is classified as L if the amino group is on the left side of the chiral carbon in a Fischer projection (excluding glycine).

    Excitatory Neurotransmitters

    • Glutamic acid and aspartic acid are primarily excitatory neurotransmitters in the central nervous system.

    Serine and Glycine in Lipid Metabolism

    • Serine and glycine are precursors in the synthesis of phospholipids.

    Amino Acids Involved in Lipid Synthesis

    • Serine and glycine are involved in the synthesis of lipids and their derivatives.

    Glutamic Acid in Neuronal Signaling

    • Glutamic acid is a primary excitatory neurotransmitter in neuron signaling.

    Tyrosine as Hormone Precursor

    • Tyrosine is a precursor for hormones like catecholamines and thyroid hormones.

    Tyrosine and Hormonal Signaling

    • Tyrosine-derived hormone precursors regulate hormonal signal transduction.

    Ornithine and Citrulline Role

    • Ornithine and citrulline are intermediate metabolites in the urea cycle.

    Inhibitory Neurotransmitter

    • γ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter.

    Peptide Bond Formation Byproduct

    • Water is a byproduct of peptide bond formation.

    Oligosaccharide Attachment in Glycoproteins

    • Oligosaccharide chains are covalently attached to specific amino acid residues in glycoproteins via glycosidic bonds.

    Common Phosphorylated Amino Acids

    • Serine and threonine are commonly phosphorylated amino acids.

    Vitamin Essentials

    • Vitamins are essential as organic coenzymes or prosthetic groups in biochemical reactions.

    Cell Cycle Checkpoint for Chromosome Attachment

    • The M checkpoint ensures all chromosomes are properly attached to spindle fibers before separation.

    Initiating tRNA in Eukaryotes

    • Met-tRNAᵢMet is the initiating tRNA in eukaryotes.

    DNA Polymerase Primer Requirement

    • DNA polymerase requires a primer because it can only add nucleotides to an existing strand.

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    Description

    This material covers morphological features of metaphase chromosomes, tRNA functionality, RNA tertiary structure, and characteristics of the pseudouridine loop (T-loop) of tRNA. It also touches on receptors for neurotransmitters and DNA replication.

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