Podcast
Questions and Answers
Which of the following is NOT typically considered a morphological feature of a metaphase chromosome?
Which of the following is NOT typically considered a morphological feature of a metaphase chromosome?
What type of molecule does tRNA transport?
What type of molecule does tRNA transport?
In neuronal signaling, what is the primary role of glutamic acid?
In neuronal signaling, what is the primary role of glutamic acid?
Which free amino acid is the precursor of hormones like thyroid hormones and catecholamines?
Which free amino acid is the precursor of hormones like thyroid hormones and catecholamines?
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How does tyrosine facilitate hormonal signal transmission?
How does tyrosine facilitate hormonal signal transmission?
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In which metabolic cycle do ornithine and citrulline serve as intermediate metabolites?
In which metabolic cycle do ornithine and citrulline serve as intermediate metabolites?
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Which non-protein amino acid is the main inhibitory neurotransmitter in the nervous system?
Which non-protein amino acid is the main inhibitory neurotransmitter in the nervous system?
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What is produced as a by-product during the formation of a peptide bond between amino acids?
What is produced as a by-product during the formation of a peptide bond between amino acids?
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How are oligosaccharide chains attached to the protein portion in glycoproteins?
How are oligosaccharide chains attached to the protein portion in glycoproteins?
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Which amino acid residues are commonly phosphorylated in proteins to form phosphoproteins?
Which amino acid residues are commonly phosphorylated in proteins to form phosphoproteins?
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What makes vitamins essential for bodily functions?
What makes vitamins essential for bodily functions?
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Which type of tRNA is responsible for initiating translation in eukaryotes?
Which type of tRNA is responsible for initiating translation in eukaryotes?
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What is the primary function of cholesterol within the cell membrane?
What is the primary function of cholesterol within the cell membrane?
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What is the role of a positive control in a PCR experiment?
What is the role of a positive control in a PCR experiment?
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What is the main purpose of cellular respiration?
What is the main purpose of cellular respiration?
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Which of the following correctly represents the sequence of events in cellular respiration?
Which of the following correctly represents the sequence of events in cellular respiration?
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Which of the following represents the correct hierarchy of RNA structure, from simplest to most complex?
Which of the following represents the correct hierarchy of RNA structure, from simplest to most complex?
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What effect does a long carbon chain have on the solubility of a carbohydrate in water?
What effect does a long carbon chain have on the solubility of a carbohydrate in water?
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What term is used to describe a carbohydrate composed of more than 10 monosaccharide molecules?
What term is used to describe a carbohydrate composed of more than 10 monosaccharide molecules?
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The sodium-potassium pump moves ions against their concentration gradients. This process is best classified as what type of membrane transport?
The sodium-potassium pump moves ions against their concentration gradients. This process is best classified as what type of membrane transport?
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A secondary lysosome is formed by the fusion of which two cellular structures?
A secondary lysosome is formed by the fusion of which two cellular structures?
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What term is used to describe a carbohydrate composed of up to 10 monosaccharide molecules?
What term is used to describe a carbohydrate composed of up to 10 monosaccharide molecules?
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What key event occurs when an mRNA molecule binds to a ribosome during translation?
What key event occurs when an mRNA molecule binds to a ribosome during translation?
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What type of proteins are produced by ribosomes that freely float in the cytoplasm?
What type of proteins are produced by ribosomes that freely float in the cytoplasm?
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What type of substances are primarily stored within amyloplasts?
What type of substances are primarily stored within amyloplasts?
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Which cellular function is primarily carried out by lysosomes?
Which cellular function is primarily carried out by lysosomes?
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How would you characterize heterochromatin during interphase?
How would you characterize heterochromatin during interphase?
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What is the first amino acid in all proteins produced by the mitochondria, chloroplasts, and bacteria?
What is the first amino acid in all proteins produced by the mitochondria, chloroplasts, and bacteria?
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What is the likely outcome if the annealing temperature is set too high in a PCR reaction?
What is the likely outcome if the annealing temperature is set too high in a PCR reaction?
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What type of ligands are "orphan" receptors intended, concerning cell signaling?
What type of ligands are "orphan" receptors intended, concerning cell signaling?
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What describes lipid rafts in cell membranes?
What describes lipid rafts in cell membranes?
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Where is the electron transport chain located in eukaryotic cells?
Where is the electron transport chain located in eukaryotic cells?
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Which of the following is the most accurate description of the main components of the cytoplasm?
Which of the following is the most accurate description of the main components of the cytoplasm?
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At which structural level is a protein said to be when it contains disulfide bridges?
At which structural level is a protein said to be when it contains disulfide bridges?
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Which of the following correctly lists types of chromosomes based on centromere position?
Which of the following correctly lists types of chromosomes based on centromere position?
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The formation of a phagosome is directly associated with which type of membrane transport?
The formation of a phagosome is directly associated with which type of membrane transport?
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Which of the nitrogenous bases listed below is a purine, characterized by its double-ring structure?
Which of the nitrogenous bases listed below is a purine, characterized by its double-ring structure?
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During the transition from interphase to metaphase, by approximately how much does DNA shorten to form chromosomes?
During the transition from interphase to metaphase, by approximately how much does DNA shorten to form chromosomes?
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What distinguishes autosomes from allosomes in a cell?
What distinguishes autosomes from allosomes in a cell?
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What role do cristae play within mitochondria?
What role do cristae play within mitochondria?
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Which of the nitrogenous bases listed below is pyrimidine, characterized by a single-ring structure?
Which of the nitrogenous bases listed below is pyrimidine, characterized by a single-ring structure?
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Which compound is essential for efficient aerobic respiration, enabling cells to break down macromolecules for energy in the form of ATP?
Which compound is essential for efficient aerobic respiration, enabling cells to break down macromolecules for energy in the form of ATP?
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In DNA condensation, which histone protein is directly involved in the formation of the 30nm fiber?
In DNA condensation, which histone protein is directly involved in the formation of the 30nm fiber?
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What is the primary function of centrosomes in a cell?
What is the primary function of centrosomes in a cell?
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During cell division, what is the kinetochore's main function?
During cell division, what is the kinetochore's main function?
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Which type of ligands are typically transported through the circulatory system to reach distant target cells?
Which type of ligands are typically transported through the circulatory system to reach distant target cells?
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What percentage of a typical cell's composition is water?
What percentage of a typical cell's composition is water?
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Which of the following phospholipid movements within the cell membrane requires enzymatic activity?
Which of the following phospholipid movements within the cell membrane requires enzymatic activity?
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What is a codon?
What is a codon?
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In PCR, what does a positive control accomplish?
In PCR, what does a positive control accomplish?
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What defines stem cells?
What defines stem cells?
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How would you describe the interphase of the cell cycle?
How would you describe the interphase of the cell cycle?
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Which interaction is NOT directly involved in the formation of the tertiary structure of RNA?
Which interaction is NOT directly involved in the formation of the tertiary structure of RNA?
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Which of the following is a key characteristic of the pseudouridine loop, also known as the T-loop, of tRNA?
Which of the following is a key characteristic of the pseudouridine loop, also known as the T-loop, of tRNA?
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What does cell signaling achieve in the context of junxacrina?
What does cell signaling achieve in the context of junxacrina?
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Which type of receptor is typically designed for neurotransmitters when a rapid cellular response is required?
Which type of receptor is typically designed for neurotransmitters when a rapid cellular response is required?
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What primarily determines the progression of the cell cycle?
What primarily determines the progression of the cell cycle?
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What are mitogens?
What are mitogens?
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What specific process drives the elongation phase of DNA replication towards irreversibility?
What specific process drives the elongation phase of DNA replication towards irreversibility?
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Following 6 PCR cycles, how many copies of the original DNA sequence are theoretically produced?
Following 6 PCR cycles, how many copies of the original DNA sequence are theoretically produced?
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What is the mechanism of action known as combinatorial control of gene expression?
What is the mechanism of action known as combinatorial control of gene expression?
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Which of the following accurately describes primary active transport?
Which of the following accurately describes primary active transport?
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What best describes the function or role of Mitogen-Activated Protein Kinase (MAPK) in cellular signaling pathways?
What best describes the function or role of Mitogen-Activated Protein Kinase (MAPK) in cellular signaling pathways?
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In addition to a protein component, what other type of molecule is characteristically found in a glycoprotein?
In addition to a protein component, what other type of molecule is characteristically found in a glycoprotein?
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How are carbohydrates composed of more than ten subunits best classified?
How are carbohydrates composed of more than ten subunits best classified?
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Which method describes how hematopoietic stem cells (HSCs) are typically collected via apheresis?
Which method describes how hematopoietic stem cells (HSCs) are typically collected via apheresis?
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What type of chemical bond is primarily responsible for the interaction between codon and anticodon bases during translation?
What type of chemical bond is primarily responsible for the interaction between codon and anticodon bases during translation?
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Within a lipid bilayer, where are the hydrophobic components of the lipids primarily located?
Within a lipid bilayer, where are the hydrophobic components of the lipids primarily located?
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Which DNA repair pathway utilizes the MGMT protein to directly remove the methyl group from the O6 position of guanine?
Which DNA repair pathway utilizes the MGMT protein to directly remove the methyl group from the O6 position of guanine?
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Which histone protein is not a component of the nucleosome core particle?
Which histone protein is not a component of the nucleosome core particle?
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During which phase of the cell cycle is the concentration of mitotic cyclin (cyclin B) typically at its highest level?
During which phase of the cell cycle is the concentration of mitotic cyclin (cyclin B) typically at its highest level?
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Under what circumstances does programmed cell death, also known as apoptosis, typically occur in multicellular organisms?
Under what circumstances does programmed cell death, also known as apoptosis, typically occur in multicellular organisms?
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During what process does the number of chromosomes of a cell become most visually distinguishable using light microscopy?
During what process does the number of chromosomes of a cell become most visually distinguishable using light microscopy?
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Which of the following molecules does tRNA primarily transport to the ribosome during protein synthesis?
Which of the following molecules does tRNA primarily transport to the ribosome during protein synthesis?
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What role does the enzyme glutamine synthetase play in neuronal signaling pathways?
What role does the enzyme glutamine synthetase play in neuronal signaling pathways?
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Which amino acid serves as a direct precursor in the biosynthesis of melanin, the pigment responsible for skin and hair color?
Which amino acid serves as a direct precursor in the biosynthesis of melanin, the pigment responsible for skin and hair color?
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How does tyrosine kinase activity primarily contribute to hormonal signal transduction?
How does tyrosine kinase activity primarily contribute to hormonal signal transduction?
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Which amino acids are classified as acidic due to their side chain properties?
Which amino acids are classified as acidic due to their side chain properties?
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In what metabolic process do ornithine and citrulline function as key intermediate metabolites?
In what metabolic process do ornithine and citrulline function as key intermediate metabolites?
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Which non-protein amino acid primarily functions as the main inhibitory neurotransmitter in the nervous system?
Which non-protein amino acid primarily functions as the main inhibitory neurotransmitter in the nervous system?
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What molecule results as a by-product during the formation of a peptide bond between amino acids?
What molecule results as a by-product during the formation of a peptide bond between amino acids?
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How are oligosaccharide chains covalently attached to proteins during the synthesis of glycoproteins?
How are oligosaccharide chains covalently attached to proteins during the synthesis of glycoproteins?
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Which amino acid residues are commonly phosphorylated in proteins to regulate their function?
Which amino acid residues are commonly phosphorylated in proteins to regulate their function?
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Why are vitamins essential for the human body?
Why are vitamins essential for the human body?
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Which checkpoint in the cell cycle ensures proper attachment of chromosomes to the spindle fibers before the initiation of anaphase?
Which checkpoint in the cell cycle ensures proper attachment of chromosomes to the spindle fibers before the initiation of anaphase?
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Which characteristic accurately describes the pseudouridine loop, also known as the T-loop, of tRNA?
Which characteristic accurately describes the pseudouridine loop, also known as the T-loop, of tRNA?
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Which type of receptors are specifically designed for neurotransmitters when a rapid cellular response is necessary?
Which type of receptors are specifically designed for neurotransmitters when a rapid cellular response is necessary?
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Which process drives the elongation phase of DNA replication towards irreversibility?
Which process drives the elongation phase of DNA replication towards irreversibility?
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What is combinatorial control of gene expression?
What is combinatorial control of gene expression?
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What kind of bonds form between the codon-anticodon bases during translation?
What kind of bonds form between the codon-anticodon bases during translation?
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What DNA repair process involves the MGMT protein, which removes the oxygen-bound methyl group O6 of guanine?
What DNA repair process involves the MGMT protein, which removes the oxygen-bound methyl group O6 of guanine?
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Which histone protein does NOT form part of the nucleosome core?
Which histone protein does NOT form part of the nucleosome core?
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How does cholesterol affect the cell membrane?
How does cholesterol affect the cell membrane?
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In which phase of the cell cycle is the concentration of mitotic cyclin (cyclin B) the highest?
In which phase of the cell cycle is the concentration of mitotic cyclin (cyclin B) the highest?
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Under what conditions does programmed cell death (apoptosis) typically occur in multicellular organisms?
Under what conditions does programmed cell death (apoptosis) typically occur in multicellular organisms?
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Which sequence of events accurately describes cellular respiration?
Which sequence of events accurately describes cellular respiration?
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What effect does a long carbon chain have on a carbohydrate's solubility in water?
What effect does a long carbon chain have on a carbohydrate's solubility in water?
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What term describes a carbohydrate containing more than 10 monosaccharide molecules?
What term describes a carbohydrate containing more than 10 monosaccharide molecules?
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What term describes a carbohydrate containing up to 10 monosaccharide molecules?
What term describes a carbohydrate containing up to 10 monosaccharide molecules?
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What type of proteins are produced by ribosomes freely floating in the cytoplasm?
What type of proteins are produced by ribosomes freely floating in the cytoplasm?
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What substances are primarily stored within amyloplasts?
What substances are primarily stored within amyloplasts?
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Which of the following accurately describes the main components of the cytoplasm?
Which of the following accurately describes the main components of the cytoplasm?
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What level of protein structure is indicated by the presence of disulfide bridges?
What level of protein structure is indicated by the presence of disulfide bridges?
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Which nitrogenous base is a purine characterized by its double-ring structure?
Which nitrogenous base is a purine characterized by its double-ring structure?
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Which of the following nitrogenous bases is classified as a pyrimidine due to its single-ring structure?
Which of the following nitrogenous bases is classified as a pyrimidine due to its single-ring structure?
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Which of the following best describes lipid rafts in cell membranes?
Which of the following best describes lipid rafts in cell membranes?
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Which of the following are types of chromosomes?
Which of the following are types of chromosomes?
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What is the difference between autosomes and allosomes?
What is the difference between autosomes and allosomes?
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What is the function of the cristae in mitochondria?
What is the function of the cristae in mitochondria?
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What is the function of centrosomes in the cell?
What is the function of centrosomes in the cell?
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What is the function of the kinetochore?
What is the function of the kinetochore?
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What percentage of a cell is water?
What percentage of a cell is water?
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What is the purpose of a positive control in PCR?
What is the purpose of a positive control in PCR?
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What are stem cells?
What are stem cells?
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What phase of the cell cycle is interphase?
What phase of the cell cycle is interphase?
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What is cell signaling in the context of junxacrine?
What is cell signaling in the context of junxacrine?
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The course of the cell cycle is primarily determined by?
The course of the cell cycle is primarily determined by?
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How many copies of DNA are produced after 6 PCR cycles?
How many copies of DNA are produced after 6 PCR cycles?
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Which of the following best describes primary active transport?
Which of the following best describes primary active transport?
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Which of the following represents the highest level of structural organization in RNA?
Which of the following represents the highest level of structural organization in RNA?
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What functions do lysosomes perform in the cell?
What functions do lysosomes perform in the cell?
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Which of the following phospholipid movements may be catalyzed by enzymes in the cell membrane?
Which of the following phospholipid movements may be catalyzed by enzymes in the cell membrane?
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How is the START codon recognized in eukaryotes during translation initiation?
How is the START codon recognized in eukaryotes during translation initiation?
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Which of the following is a component of a glycolipid?
Which of the following is a component of a glycolipid?
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Which of the following DNA strands can form a DNA duplex by pairing with itself at each position?
Which of the following DNA strands can form a DNA duplex by pairing with itself at each position?
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What is the role of EDTA in blood collection and laboratory experiments?
What is the role of EDTA in blood collection and laboratory experiments?
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What is a key safety consideration when using Midori Green in molecular biology experiments?
What is a key safety consideration when using Midori Green in molecular biology experiments?
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What is the primary use of Midori Green in molecular biology?
What is the primary use of Midori Green in molecular biology?
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What is the structure of a DNA helix?
What is the structure of a DNA helix?
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In PCR, what is the purpose of the denaturation step?
In PCR, what is the purpose of the denaturation step?
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What is the function of mitochondria in human cells?
What is the function of mitochondria in human cells?
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What is the typical annealing temperature used in PCR?
What is the typical annealing temperature used in PCR?
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What is the main feature of the structure of biological membranes?
What is the main feature of the structure of biological membranes?
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What is deoxyribonucleic acid (DNA) made of?
What is deoxyribonucleic acid (DNA) made of?
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Agarose is made of which of the following?
Agarose is made of which of the following?
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Flashcards
Metaphase Chromosome Features
Metaphase Chromosome Features
Characteristics including banding pattern, centromere position, number, and length.
Banding Pattern
Banding Pattern
The specific pattern of light and dark bands on a chromosome during metaphase.
Centromere Position
Centromere Position
The location of the centromere on the chromosome, affecting its behavior during cell division.
Number of Chromosomes
Number of Chromosomes
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tRNA Function
tRNA Function
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Glutamic acid
Glutamic acid
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Tyrosine
Tyrosine
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Hormonal signal transmission
Hormonal signal transmission
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Urea cycle
Urea cycle
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GABA
GABA
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Peptide bond formation
Peptide bond formation
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Glycoprotein attachment
Glycoprotein attachment
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Phosphoproteins
Phosphoproteins
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Vitamins
Vitamins
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M checkpoint
M checkpoint
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Peptides
Peptides
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Codons
Codons
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Tertiary structure of RNA
Tertiary structure of RNA
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Pseudouridine loop (T-loop)
Pseudouridine loop (T-loop)
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Ionotropic receptors
Ionotropic receptors
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PPi in DNA replication
PPi in DNA replication
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Combinatorial control
Combinatorial control
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MAPK
MAPK
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Glycoprotein components
Glycoprotein components
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Polysaccharides
Polysaccharides
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Codon-anticodon interactions
Codon-anticodon interactions
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Nucleotide excision repair
Nucleotide excision repair
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Metacentric chromosomes
Metacentric chromosomes
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Anaphase of mitosis
Anaphase of mitosis
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Cell as the smallest living unit
Cell as the smallest living unit
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Sodium-potassium pump
Sodium-potassium pump
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Secondary lysosome
Secondary lysosome
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mRNA and ribosome interaction
mRNA and ribosome interaction
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Lysosome functions
Lysosome functions
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Heterochromatin in interphase
Heterochromatin in interphase
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Orphan receptors
Orphan receptors
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Electron transport chain location
Electron transport chain location
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Phagosome formation
Phagosome formation
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DNA shortening in metaphase
DNA shortening in metaphase
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Aerobic respiration
Aerobic respiration
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Chromatin formation
Chromatin formation
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Biological membranes structure
Biological membranes structure
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PCR denaturation step
PCR denaturation step
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Role of mitochondria
Role of mitochondria
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Lipid Rafts
Lipid Rafts
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Types of Chromosomes
Types of Chromosomes
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Autosomes vs Allosomes
Autosomes vs Allosomes
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Cristae Function
Cristae Function
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Centrosome Function
Centrosome Function
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Kinetochore
Kinetochore
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Cell Water Percentage
Cell Water Percentage
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Positive Control in PCR
Positive Control in PCR
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Stem Cells
Stem Cells
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Interphase
Interphase
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Cell Cycle Determination
Cell Cycle Determination
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Mitogens
Mitogens
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Primary Active Transport
Primary Active Transport
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Totipotent Stem Cells
Totipotent Stem Cells
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Structure of DNA
Structure of DNA
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Cellular Respiration Purpose
Cellular Respiration Purpose
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Events in Cellular Respiration
Events in Cellular Respiration
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Long Carbon Chain in Carbohydrates
Long Carbon Chain in Carbohydrates
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Oligosaccharide
Oligosaccharide
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Function of Cytoplasmic Ribosomes
Function of Cytoplasmic Ribosomes
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Amyloplasts
Amyloplasts
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First Amino Acid in Proteins
First Amino Acid in Proteins
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PCR Annealing Temperature
PCR Annealing Temperature
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Degeneracy of Genetic Code
Degeneracy of Genetic Code
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Amino Acid with Thiol Group
Amino Acid with Thiol Group
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Excitatory Neurotransmitters
Excitatory Neurotransmitters
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Role of Serine and Glycine
Role of Serine and Glycine
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Cyclic Non-Polar Amino Acid
Cyclic Non-Polar Amino Acid
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Non-morphological feature of Chromosome
Non-morphological feature of Chromosome
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Quaternary structure of RNA
Quaternary structure of RNA
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Heterochromatin
Heterochromatin
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Aerobic Respiration Component
Aerobic Respiration Component
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DNA Duplex Formation
DNA Duplex Formation
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PCR Denaturation Step Purpose
PCR Denaturation Step Purpose
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Structure of DNA Helix
Structure of DNA Helix
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Start Codon Recognition
Start Codon Recognition
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Glycolipid Component
Glycolipid Component
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Glutamic Acid Function
Glutamic Acid Function
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Tyrosine Hormone Precursor
Tyrosine Hormone Precursor
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Tyrosine's Role in Hormones
Tyrosine's Role in Hormones
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Ornithine and Citrulline
Ornithine and Citrulline
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Peptide Bond By-product
Peptide Bond By-product
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Phosphorylated Amino Acids
Phosphorylated Amino Acids
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Vitamins' Essential Role
Vitamins' Essential Role
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Mitosis Checkpoint
Mitosis Checkpoint
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DNA replication irreversible process
DNA replication irreversible process
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Combinatorial control of gene expression
Combinatorial control of gene expression
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Mitogen-activated Protein Kinase (MAPK)
Mitogen-activated Protein Kinase (MAPK)
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Polysaccharides definition
Polysaccharides definition
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Mitosis phase with chromosome separation
Mitosis phase with chromosome separation
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Cellular apoptosis triggers
Cellular apoptosis triggers
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Histone outside nucleosome core
Histone outside nucleosome core
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Hydrophobic components of lipid bilayer
Hydrophobic components of lipid bilayer
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Cyclin B concentration in cell cycle
Cyclin B concentration in cell cycle
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Sequence of Events in Cellular Respiration
Sequence of Events in Cellular Respiration
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Long Carbon Chain Effect in Carbohydrates
Long Carbon Chain Effect in Carbohydrates
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Nitrogenous Bases Structures
Nitrogenous Bases Structures
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Study Notes
Morphological Features of Metaphase Chromosomes
- Banding pattern, position of the centromere, and chromosome length are morphological features.
- Number of chromosomes is not a morphological feature; it's a numerical characteristic.
tRNA Transport
- tRNA transports amino acids, not peptides, codons, or proteins.
RNA Tertiary Structure Interactions
- Not all interactions are involved: Formation of an A-type helix, interactions of arm stems, and binding of basic proteins/ions are involved.
- Formation of non-canonical pairs/triplets is also a critical interaction.
- Creation of an A-type helix, Interactions of arm stems with other arm stems, Binding of basic proteins or ions that neutralize the negative charge of RNA are involved in the formation of a tertiary structure of RNA. The exclusionary statement is the formation of non-canonical pairs/triplets.
Pseudouridine Loop (T-loop) of tRNA
- The T-loop contains an unpaired CCA sequence at the 3' end. It does not contain a D-loop, mobilize the tRNA directly, or contain the ribosome binding site.
Receptors for Neurotransmitters
- Ionotropic (ion-channel-coupled) receptors are designed for rapid responses to neurotransmitters.
DNA Replication Irreversibility
- Hydrolysis of pyrophosphate (PPi) to inorganic phosphate (Pi) during DNA replication makes the process irreversible.
Combinatorial Control of Gene Expression
- Combinatorial control involves the coordinated action of multiple transcriptional regulators for proper gene expression.
Mitogen-Activated Protein Kinase (MAPK)
- MAPK is a serine-threonine kinase that plays a crucial role in cellular signaling pathways, receiving the final phosphorylation signal from Ras activation.
- MAPK is the final serine-threonine kinase following the activation of Ras protein.
Glycoprotein Components
- Oligosaccharides are a component of glycoproteins, in addition to the protein itself. Lipids and metal ions aren't components of glycoproteins.
Carbohydrate Subunit Classification
- Carbohydrates with more than 10 subunits are called polysaccharides.
HSC Collection via Apheresis
- HSCs are collected using fluorescent antibodies that specifically target the CD34 surface protein.
Codon-Anticodon Bonds
- Hydrogen bonds form between codon-anticodon bases.
Lipid Bilayer Hydrophobic Components
- Hydrophobic components are located inside the lipid bilayer.
DNA Repair via MGMT
- Direct repair (DR) involving the MGMT protein removes the oxygen-bound methyl group from O6 of guanine.
Histone Not in Nucleosome Core
- Histone H1 is not part of the nucleosome core.
Mitotic Cyclin Concentration
- The concentration of mitotic cyclin (cyclin B) is highest in the M phase of the cell cycle.
Programmed Cell Death (Apoptosis)
- Apoptosis occurs when a cell-death intracellular program is initiated, and it's not limited to embryonic development or requiring energy.
Amino Acids in Human Proteins
- There are 20 basic amino acids found in human proteins.
Protein Secondary Structure
- The hydrogen bond between the NH and CO of amino acid residues i and i+3 stabilizes the α-helix.
Interphase Phases
- Interphase includes the G1, S, and G2 phases.
Nucleotide Excision Repair
- Nucleotide excision repair involves XPG and XPF endonucleases cutting DNA strands around the damage, helicases unwinding the DNA, the damaged DNA being removed, and DNA polymerase and ligase synthesizing and sealing the new DNA strand.
- Step that does NOT occur in nucleotide excision repair is the "Removal of the damaged nitrogenous base by specific DNA glycosylase".
Chromosome Arm Length
- A metacentric chromosome has arms of equal length.
Smallest Living Unit
- A cell is the smallest living unit.
Chromosome Position Between Divisions
- Chromosomes occupy distinct territories in the interphase nucleus, not the nucleolus.
RNA Structure with Apical Loops
- Apical loops (hairpens) exist in the secondary RNA structure.
Sodium-Potassium Pump
- The sodium-potassium pump is categorized as primary active transport.
Secondary Lysosome Formation
- A secondary lysosome is the result of the fusion of a primary lysosome with a phagosome.
mRNA and Ribosomes
- When mRNA attaches to a ribosome, the large and small subunits join and begin translation, not separating or changing sedimentation rate.
Lysosome Function
- Lysosomes break down carbohydrates, proteins, fats, and nucleic acids.
Heterochromatin in Interphase
- Heterochromatin is genetically inactive, stains dark under the microscope, and is in a condensed state during interphase.
Orphan Receptors and Ligands
- "Orphan" receptors are intended for unknown ligands.
Electron Transport Chain Location
- The electron transport chain occurs in the inner membrane of the mitochondria.
Membrane Transport and Phagosome Formation
- Phagosome formation utilizes phagocytosis, which is a type of endocytosis.
Interphase DNA Shortening
- Interphase DNA condenses approximately 10,000 times to form metaphase chromosomes.
Aerobic Respiration and Compound
- Oxygen is necessary for aerobic respiration.
DNA Condensation with Histone H1
- Histone H1 is involved in the formation of the 30nm fiber during DNA condensation.
Circulatory Transport Ligands
- Hormones and neurotransmitters are transported through the circulatory system.
Phospholipid Movement
- Lateral diffusion and rotational motion of phospholipids in cell membranes are catalyzed by enzymes; not transverse “flip-flop” movement.
Codon Definition
- A codon is three nucleotides of RNA.
START Codon Recognition in Eukaryotes
- The START codon is recognized through the binding of the small ribosomal subunit, mRNA, and initiator tRNA. This involves scanning from the 5' end.
Glycolipid Component
- Sphingosine is a component of glycolipids.
DNA Sequence for Duplex Formation
- 5’-AAGCCGTT-3’ can form a DNA duplex with itself.
EDTA Role in Blood Analysis
- EDTA is an anticoagulant that prevents blood clotting by binding calcium ions.
Midori Green Safety
- Midori Green is toxic and requires safety precautions like gloves and eye protection.
Midori Green Use
- Midori Green stains DNA for visualization during gel electrophoresis.
DNA Helix Structure
- DNA forms a double-stranded, right-handed helix.
PCR Denaturation Step
- The denaturation step in PCR separates the DNA strands by breaking hydrogen bonds.
Mitochondria Function
- Mitochondria generate ATP through cellular respiration.
PCR Annealing Temperature
- The annealing temperature typically ranges from 50-65°C in PCR.
Biological Membrane Structure
- Biological membranes consist of a fluid bilayer of phospholipids with embedded proteins.
DNA Components
- DNA is composed of nucleotides with a phosphate group, sugar, and nitrogenous base.
Agarose Composition
- Agarose is a polysaccharide derived from seaweed.
Lipid Rafts
- Lipid rafts are lipid-rich regions in cell membranes involved in signaling and trafficking.
Chromosome Types
- Chromosomes can be prokaryotic or eukaryotic; types include metacentric, submetacentric, acrocentric, and telocentric.
Autosomes vs. Allosomes
- Autosomes are non-sex chromosomes, and allosomes are sex chromosomes.
Mitochondria Cristae Function
- Cristae increase the surface area of the mitochondria, which is important for ATP production.
Centrosome Function
- Centrosomes organize microtubules and form the mitotic spindle.
Kinetochore Function
- Kinetochores attach chromosomes to the spindle during cell division.
Cell Water Content
- Cells are typically 50-70% water.
PCR Positive Control Role
- A positive control in PCR ensures the process is working by using a known sample.
Stem Cells
- Stem cells are cells that can divide and differentiate into various cell types.
Interphase Cell Cycle Phase
- Interphase is the phase of the cell cycle where the cell is preparing for division, growing, or completing other essential activities. Not actively dividing or completing mitosis.
Cell Signaling
- Cell signaling, in the context of junxacrina, is the process by which cells respond to external signals to regulate growth and other processes; not directly about junxacrina protein synthesis.
Cell Cycle Regulation
- The progression through the cell cycle is primarily controlled by cyclin-dependent protein kinases (CDKs), with cyclins needed for proper enzymatic activity.
Mitogens
- Mitogens are extracellular signal molecules that stimulate cell proliferation.
PCR Cycle DNA Copies
- After 6 PCR cycles, there are 64 copies of DNA.
Primary Active Transport
- Primary active transport moves molecules from low to high concentration using energy from ATP.
Totipotent Stem Cells
- Totipotent stem cells can form all cell types in the body, including extra-embryonic tissues.
Protein Kinase C (PKC)
- PKC adds phosphate groups to proteins, regulating cellular processes.
DNA Structure
- DNA is a double-stranded molecule composed of nucleotides, with each nucleotide containing a nitrogenous base, a five-carbon sugar, and a phosphate group.
Amino Acid Core Structure
- The core structure of an amino acid has a central carbon atom bonded to an amino group, carboxyl group, and a hydrogen atom (and a side chain that varies).
Amino Acid Backbone
- The backbone of an amino acid, neglecting hydrogen atoms, is C-C-N.
Number of Amino Acids
- There are 20 standard amino acids found in natural proteins.
RNA Concentration in a Cell
- RNA concentration in a typical cell is estimated at 1-5% or 30-40%.
Dominant RNA Type
- Ribosomal RNA (rRNA) is the most dominant type of RNA in a cell.
Potassium and Sodium Ion Concentration
- Potassium (K⁺) ions are most concentrated intracellularly, and sodium (Na⁺) ions are most concentrated extracellularly.
Cholesterol in Cell Membranes
- Cholesterol modulates membrane fluidity, helping to maintain flexibility and stability.
PCR Positive Control Function
- A positive control in PCR verifies the analysis process is functioning by using a known sample.
Cellular Respiration Purpose
- Cellular respiration breaks down glucose to produce ATP.
Cellular Respiration Sequence
- Cellular respiration follows the sequence: Glycolysis → Krebs cycle → Electron transport chain.
Carbohydrate Chain Length and Solubility
- Longer carbon chains in carbohydrates make them less soluble in water.
Carbohydrate Classification by Subunits
- Carbohydrates with more than 10 subunits are called polysaccharides; those with up to 10 are oligosaccharides.
Cytoplasm Ribosome Function
- Ribosomes floating freely in the cytoplasm produce proteins that function within the cell's cytosol.
Amyloplast Contents
- Amyloplasts primarily contain starch grains.
First Amino Acid in Proteins
- The first amino acid in proteins produced by mitochondria, chloroplasts, and bacteria is N-formylmethionine.
PCR Annealing Temperature Impacts
- Setting the annealing temperature too high in PCR prevents primers from binding to the template DNA.
Cytoplasm Components
- The cytoplasm is primarily composed of water, salts, and organic molecules.
Disulfide Bridges in Protein Structure
- Proteins with disulfide bridges primarily exhibit tertiary structure.
Nitrogenous Base Types
- Adenine and guanine are double-ring (purine) bases; cytosine, thymine, and uracil (in RNA) are single-ring (pyrimidine) bases.
Genetic Code Degeneracy
- Degeneracy of the genetic code means that multiple codons can specify the same amino acid, mostly differing at the third nucleotide position.
Thiol-Containing Amino Acid
- Cysteine contains a thiol (-SH) group.
Hydroxyl-Containing Amino Acid
- Threonine contains a hydroxyl (-OH) group.
Nonpolar Aliphatic Amino Acid
- Leucine is a nonpolar, aliphatic amino acid.
Cyclic Side Chain Amino Acid
- Proline has a cyclic side chain.
α-Amino Acid Classification
- α-amino acids (except glycine) are classified as D or L based on the orientation of the amino group relative to the chiral carbon in a Fischer projection.
L-Amino Acid Designation
- An amino acid is classified as L if the amino group is on the left side of the chiral carbon in a Fischer projection (excluding glycine).
Excitatory Neurotransmitters
- Glutamic acid and aspartic acid are primarily excitatory neurotransmitters in the central nervous system.
Serine and Glycine in Lipid Metabolism
- Serine and glycine are precursors in the synthesis of phospholipids.
Amino Acids Involved in Lipid Synthesis
- Serine and glycine are involved in the synthesis of lipids and their derivatives.
Glutamic Acid in Neuronal Signaling
- Glutamic acid is a primary excitatory neurotransmitter in neuron signaling.
Tyrosine as Hormone Precursor
- Tyrosine is a precursor for hormones like catecholamines and thyroid hormones.
Tyrosine and Hormonal Signaling
- Tyrosine-derived hormone precursors regulate hormonal signal transduction.
Ornithine and Citrulline Role
- Ornithine and citrulline are intermediate metabolites in the urea cycle.
Inhibitory Neurotransmitter
- γ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter.
Peptide Bond Formation Byproduct
- Water is a byproduct of peptide bond formation.
Oligosaccharide Attachment in Glycoproteins
- Oligosaccharide chains are covalently attached to specific amino acid residues in glycoproteins via glycosidic bonds.
Common Phosphorylated Amino Acids
- Serine and threonine are commonly phosphorylated amino acids.
Vitamin Essentials
- Vitamins are essential as organic coenzymes or prosthetic groups in biochemical reactions.
Cell Cycle Checkpoint for Chromosome Attachment
- The M checkpoint ensures all chromosomes are properly attached to spindle fibers before separation.
Initiating tRNA in Eukaryotes
- Met-tRNAᵢMet is the initiating tRNA in eukaryotes.
DNA Polymerase Primer Requirement
- DNA polymerase requires a primer because it can only add nucleotides to an existing strand.
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Description
This material covers morphological features of metaphase chromosomes, tRNA functionality, RNA tertiary structure, and characteristics of the pseudouridine loop (T-loop) of tRNA. It also touches on receptors for neurotransmitters and DNA replication.