Cell Biology: Chromosomes, tRNA, and Mitosis
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Questions and Answers

Which of the following is NOT a morphological feature of a metaphase chromosome?

  • Position of the centromere
  • Number of chromosomes (correct)
  • Banding pattern
  • Chromosome length
  • What type of molecule is transported by tRNA?

  • Proteins
  • Peptides
  • Codons
  • Amino acids (correct)
  • Which interaction is least likely to be directly involved in the formation of the tertiary structure of RNA?

  • Interactions of arm stems with other arm stems
  • Formation of non-canonical pairs or triplets
  • Binding of basic proteins or ions that neutralize the negative charge of RNA (correct)
  • Creation of an A-type helix
  • Which characteristic is associated with the pseudouridine loop (T-loop) of tRNA?

    <p>It contains the site where the tRNA binds to the ribosome (B)</p> Signup and view all the answers

    Which type of receptor is best suited for neurotransmitters when a rapid cellular response is required?

    <p>ionotropic (ion-channel-coupled) receptors (A)</p> Signup and view all the answers

    What enzymatic process directly ensures that DNA replication elongation proceeds in an irreversible manner?

    <p>Hydrolysis of pyrophosphate (PPi) to inorganic phosphate PP (C)</p> Signup and view all the answers

    What defines combinatorial control of gene expression in eukaryotes?

    <p>This is a process that involves the use of a group of transcriptional regulators working together to express a gene correctly (A)</p> Signup and view all the answers

    What is the primary function of Mitogen-activated Protein Kinase (MAPK) in a signaling cascade?

    <p>The final serine-threonine kinase following the activation of Ras protein (A)</p> Signup and view all the answers

    A chromosome with arms of equal length is classified as what type?

    <p>Metacentric (C)</p> Signup and view all the answers

    During which phase of mitosis do sister chromatids separate and migrate to opposite poles of the cell?

    <p>Anaphase (B)</p> Signup and view all the answers

    What is considered the basic structural and functional unit of all known living organisms?

    <p>Cell (C)</p> Signup and view all the answers

    How are chromosomes arranged within the interphase nucleus between cell divisions?

    <p>They occupy distinct areas in the interphase nucleus (A)</p> Signup and view all the answers

    In RNA structure, where are hairpin loops (apical loops) typically found?

    <p>Secondary RNA structure (D)</p> Signup and view all the answers

    The sodium-potassium pump, which transports ions against their concentration gradients using ATP, is an example of what kind of membrane transport?

    <p>Primary active transport (A)</p> Signup and view all the answers

    What is formed by the fusion of a primary lysosome with a phagosome?

    <p>A primary lysosome with a phagosome (A)</p> Signup and view all the answers

    What happens immediately after an mRNA molecule binds to a ribosome?

    <p>The large subunit and small subunit become conjoined to begin translation (B)</p> Signup and view all the answers

    A scientist discovers a new carbohydrate composed of 25 monosaccharide subunits. How should this carbohydrate be classified?

    <p>Polysaccharide (B)</p> Signup and view all the answers

    A researcher needs to isolate hematopoietic stem cells (HSCs) from a blood sample using apheresis. Which method is most effective for selectively capturing HSCs?

    <p>Using fluorescent antibodies selective for the CD34 surface protein (D)</p> Signup and view all the answers

    During protein synthesis, what type of bond is primarily responsible for maintaining the interaction between a tRNA anticodon and an mRNA codon?

    <p>Hydrogen bonds (D)</p> Signup and view all the answers

    Where are the hydrophobic fatty acid tails of phospholipids primarily located within the structure of a lipid bilayer?

    <p>Inside the lipid bilayer (D)</p> Signup and view all the answers

    A mutation results in the addition of a methyl group to the O6 position of guanine. Which DNA repair mechanism directly reverses this type of damage?

    <p>Direct repair (DR) (C)</p> Signup and view all the answers

    Which histone protein is NOT part of the nucleosome core particle but instead functions to stabilize the chromatin structure by binding to the linker DNA?

    <p>H1 (D)</p> Signup and view all the answers

    A researcher is studying cell cycle regulation and observes that mitotic cyclin (cyclin B) levels are highest in a particular phase. In which phase of the cell cycle would the researcher observe the highest concentration of cyclin B?

    <p>M phase (B)</p> Signup and view all the answers

    Under what cellular circumstances is the process of programmed cell death (apoptosis) initiated and executed?

    <p>When the activation of a cell-death intracellular program is initiated (A)</p> Signup and view all the answers

    What compound is essential for aerobic respiration, enabling cells to break down macromolecules stepwise to produce ATP?

    <p>Oxygen (C)</p> Signup and view all the answers

    During DNA condensation, which level of chromatin organization directly involves histone H1?

    <p>Formation of the 30nm fiber (A)</p> Signup and view all the answers

    What types of ligands are transported through the circulatory system to reach target cells?

    <p>Neurotransmitters and hormones, like adrenalin (epinephrine) (B)</p> Signup and view all the answers

    Which of the following phospholipid movements within a cell membrane requires enzymatic catalysis?

    <p>Transverse &quot;flip-flop&quot; movement (A)</p> Signup and view all the answers

    What is the composition of a codon?

    <p>Three nucleotides of RNA (A)</p> Signup and view all the answers

    How do eukaryotes recognize the START codon during translation initiation?

    <p>Through the binding of the small unit of ribosome, mRNA and initiator tRNA (D)</p> Signup and view all the answers

    Which molecule is a key component of glycolipids, contributing to their structure and function in cell membranes?

    <p>Sphingosine (A)</p> Signup and view all the answers

    Which of the following DNA strands can form a DNA duplex by pairing with itself at each position?

    <p>5'-AAGCGCTT-3' (A)</p> Signup and view all the answers

    What is the primary function of amyloplasts within plant cells?

    <p>Storing carbohydrates in the form of starch grains. (C)</p> Signup and view all the answers

    Which amino acid is uniquely utilized as the initiation amino acid in protein synthesis within mitochondria and chloroplasts?

    <p>N-formylmethionine (B)</p> Signup and view all the answers

    During PCR, what is the likely outcome if the annealing temperature is set too high?

    <p>Primers failing to bind effectively to the DNA template. (A)</p> Signup and view all the answers

    Which structural level is directly associated with the presence of disulfide bridges in proteins?

    <p>Tertiary structure (A)</p> Signup and view all the answers

    A nitrogenous base containing two rings is classified as which of the following?

    <p>Purine (C)</p> Signup and view all the answers

    Considering the degeneracy of the genetic code, what is its primary implication for amino acid encoding?

    <p>Multiple codons can specify the same amino acid. (C)</p> Signup and view all the answers

    Which of the following is a key characteristic that must be present for DNA polymerase function?

    <p>Requirement for a primer to initiate DNA synthesis. (C)</p> Signup and view all the answers

    How do met-tRNAm and fmet-tRNAf differ in their roles during protein synthesis?

    <p>met-tRNAm participates in elongation, while fmet-tRNAf participates in initiation. (C)</p> Signup and view all the answers

    Which of the following cellular components directly stimulates the production of cyclins, leading to cell division?

    <p>Intercellular signals that stimulate the production of cyclins. (B)</p> Signup and view all the answers

    Following 6 PCR cycles, how many copies of a specific DNA sequence would ideally be produced, assuming 100% efficiency?

    <p>64 (D)</p> Signup and view all the answers

    The sodium-potassium pump is a critical component of cellular function. What type of transport does it utilize?

    <p>Primary active transport (C)</p> Signup and view all the answers

    Which term accurately describes embryonic stem cells with the ability to differentiate into any cell type, including extraembryonic tissues?

    <p>Totipotent/Omnipotent (D)</p> Signup and view all the answers

    Which of the following molecules is NOT directly targeted for destruction or modification by a protein kinase?

    <p>Lipids (D)</p> Signup and view all the answers

    Considering the structural components of DNA, what is the primary linkage pattern of the amino acid backbone in a polypeptide chain?

    <p>N-C-C (A)</p> Signup and view all the answers

    Within a typical cell, ribosomal RNA (rRNA) constitutes the most significant percentage of total RNA. Approximately what percentage does rRNA represent?

    <p>70-80% (B)</p> Signup and view all the answers

    What is the primary role of cholesterol within the cell membrane?

    <p>To hold phospholipids together, influencing membrane fluidity. (D)</p> Signup and view all the answers

    Flashcards

    Metaphase Chromosome Features

    Banding pattern, centromere position, length; number is not a feature.

    tRNA Function

    tRNA transports amino acids to ribosomes during protein synthesis.

    Tertiary Structure of RNA

    Tertiary structure stability does not involve binding basic proteins or ions to neutralize charge.

    Pseudouridine Loop in tRNA

    This loop is crucial for tRNA's ribosome binding site.

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    Ionotropic Receptors

    Receptors designed for rapid responses to neurotransmitters.

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    DNA Replication Irreversibility

    Hydrolysis of pyrophosphate makes DNA replication irreversible.

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    Combinatorial Control of Gene Expression

    A process using multiple transcriptional regulators together for gene expression.

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    Mitogen-activated Protein Kinase (MAPK)

    Final kinase in the signaling pathway activated by Ras protein.

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    Polysaccharides

    Carbohydrates made up of more than 10 subunits.

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    CD34 surface protein

    A marker used to collect hematopoietic stem cells via apheresis.

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    Hydrogen bonds

    Non-covalent bonds that form between codon-anticodon bases.

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    Lipid bilayer hydrophobic region

    The inside of the lipid bilayer contains hydrophobic components.

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    Direct repair (DR)

    DNA repair process involving the MGMT protein to remove methyl groups.

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    Histone H1

    The histone protein that does not form part of the nucleosome core.

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    Mitotic phase M

    Phase in the cell cycle when concentration of cyclin B is highest.

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    Apoptosis conditions

    Programmed cell death initiated by specific intracellular signals.

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    Metacentric chromosome

    A chromosome with arms of equal length.

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    Anaphase in mitosis

    The phase where sister chromosomes separate.

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    Smallest living unit

    The cell is the smallest living unit on Earth.

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    Interphase nucleus occupation

    Chromosomes occupy distinct areas in the interphase nucleus.

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    Secondary RNA structure

    Structure of RNA that includes apical loops (hairpins).

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    Primary active transport

    Type of membrane transport like the sodium-potassium pump.

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    Secondary lysosome formation

    A secondary lysosome results from a primary lysosome combining with a phagosome.

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    Lysosome functions

    Lysosomes break down carbohydrates, proteins, fats, and nucleic acids.

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    Necessary compound for aerobic respiration

    Oxygen is required for aerobic respiration to produce ATP.

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    Histone H1 involvement

    Histone H1 is directly involved in the formation of the 30nm fiber in DNA condensation.

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    Ligands in the circulatory system

    Neurotransmitters and hormones are transported through the circulatory system.

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    Phospholipid movement enzyme activity

    Enzymes can catalyze the transverse 'flip-flop' movement of phospholipids.

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    What is a codon?

    A codon is a sequence of three nucleotides of RNA that codes for an amino acid.

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    Start codon recognition in translation

    The START codon is recognized through the binding of the small ribosomal unit, mRNA, and initiator tRNA.

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    Component of a glycolipid

    Sphingosine is a component of glycolipids in cell membranes.

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    Cyclin-dependent protein kinases (cdk)

    The cell cycle is determined by cyclin-dependent protein kinases becoming active with cyclins.

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    Mitosis Regulation Genes

    Genes that control the process of mitosis and cell division.

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    Extracellular Signal Molecules

    Molecules that stimulate cell proliferation from outside the cell.

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    Cyclins and Intercellular Signals

    Signals that lead to the production of cyclins, which activate cell division.

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    Structure of DNA

    DNA is a polymer made of nucleotides with nitrogenous bases, sugar, and phosphate group.

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    Amino Acid Structure

    Amino acids have a central carbon, an amino group, a carboxyl group, and a hydrogen atom.

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    Prevalent RNA Type

    rRNA comprises 70-80% of all RNA in cells, important for protein synthesis.

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    Cholesterol's Role in Cells

    Cholesterol maintains the integrity of cell membranes by holding phospholipids together.

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    Free Ribosome Function

    Ribosomes in the cytoplasm produce proteins that function within the cytosol.

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    Amyloplast Composition

    Amyloplasts contain carbohydrates in the form of starch grains.

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    First Amino Acid in Protein

    The first amino acid in proteins produced by mitochondria and chloroplasts is N-formylmethionine.

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    High PCR Annealing Temperature

    If the annealing temperature is too high, primers will not bind to the template.

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    Cytoplasm Components

    Cytoplasm is composed of water, salts, and various organic molecules.

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    Protein Structure with Sulfide Bridge

    A protein bound with a sulfide bridge has a tertiary structure.

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    Purines vs. Pyrimidines

    Purines are double-ring structures; pyrimidines are single-ring structures.

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    Peptide Bond Formation

    A peptide bond forms by a condensation reaction between an amino group and a carboxyl group, releasing water.

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    Types of Glycoproteins

    Glycoproteins contain covalently bound oligosaccharides, either straight chain or branched.

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    Study Notes

    Biology Exam Questions - Summary

    • Question 1: Metaphase chromosome features:

      • Banding pattern is a morphological feature
      • Position of the centromere is a morphological feature
      • Number of chromosomes is not a morphological feature
      • Chromosome length is a morphological feature
    • Question 2: tRNA transport:

      • tRNA transports amino acids
    • Question 3: RNA tertiary structure interactions:

      • Creation of A-type helix is involved in tertiary structure formation
      • Interactions of arm stems are involved in tertiary structure formation
      • Binding of basic proteins/ions is NOT involved in tertiary structure formation.
    • Question 4: Pseudouridine loop (T-loop) of tRNA:

      • Contains the site where tRNA binds to the ribosome
      • Contains a D loop containing dihydrouridine
      • Contains an unpaired CCA sequence at the 3' end.
      • Mobilizes tRNA during protein synthesis
    • Question 5: Neurotransmitter receptors:

      • Ionotropic (ion-channel-coupled) receptors are for rapid responses
      • Receptors for lipophilic metabotropic hormones are not rapid
      • Catalytic receptors are not rapid
      • G-proteins coupled receptors are for rapid responses
    • Question 6: DNA replication irreversibility:

      • Hydrolysis of pyrophosphate (PPi) to inorganic phosphate (Pi) makes DNA replication irreversible.
    • Question 7: Combinatorial control of gene expression:

      • Involves multiple transcriptional regulators working together for gene expression, not just one.
    • Question 8: Mitogen-activated Protein Kinase (MAPK):

      • The final serine-threonine kinase following Ras protein activation
      • Involved in the exchange of GDP for GTP in the Ras protein.
      • Result of dephosphorylation of serine/threonine residues from other kinases.
      • The attachment of adaptor protein to a tyrosine residue.
    • Question 9: Glycoprotein component:

      • Lipids can be a component of a glycoprotein.
      • Metal ions can be part
      • Oligosaccharides can be part.
    • Question 10: Carbohydrates with more than 10 subunits:

      • Polysaccharides are carbohydrates with more than 10 subunits
    • Question 11: HSC collection via apheresis:

      • Using fluorescent antibodies selective for the CD34 surface protein.
    • Question 12: Codon-anticodon bonds:

      • Hydrogen bonds form between codon-anticodon bases
    • Question 13: Lipid bilayer segments:

      • Hydrophobic components are inside the lipid bilayer
    • Question 14: DNA repair process using MGMT protein:

      • Direct repair (DR) using MGMT protein
    • Question 15: Histone protein not in nucleosome core:

      • H1
    • Question 16: Mitotic cyclin concentration highest phase:

      • High concentration occurs in phase M.s
    • Question 17: Programmed cell death (apoptosis) conditions:

      • Apoptosis occurs during embryonic development and other circumstances.
    • Question 18: Basic amino acids in human proteins:

      • Number of basic amino acids in human proteins
    • Question 19: Secondary structure stabilized by hydrogen bonds:

      • β-turns
    • Question 20: Interphase phases:

      • G1, S, and G2 phases.
    • Question 21: Nucleotide excision repair steps:

      • DNA strand cutting, base removal and synthesis of a new DNA strand.
    • Question 22: Chromosome arm length equality:

      • Metacentric chromosomes have equal arm lengths.
    • Question 23: Sister chromosome separation phase:

      • Separation occurs during anaphase.
    • Question 24: Smallest living unit on Earth:

      • The cell.
    • Question 25: Chromosome locations between divisions:

      • Chromosomes occupy distinct areas in the interphase nucleus.
    • Question 26: RNA structure with apical loops:

      • Apical loops exist in the secondary structure of RNA.
    • Question 27: Membrane transport classification:

      • Sodium-potassium pump is classified as primary active transport
    • Question 28: Phagosome formation:

      • Secondary active transport is associated with phagosome formation.
    • Question 29: mRNA attachment to ribosome:

      • mRNA attaching to a ribosome causes conformational change to the large subunit, allowing it to bind to the ER.
    • Question 30: Lysosome functions:

      • Breakdown of carbohydrates, proteins, fats and nucleic acids
      • Sorting of transport vesicles.
      • Transport of materials to the Golgi apparatus, Storage of excess macromolecules.
    • Question 31: Heterochromatin in interphase:

      • Genetically inactive and densely stained.
    • Question 32: Ligands for orphan receptors:

      • Includes unknown ligands, active vitamin D3 and active vitamin A (retinol) and lipophilic hormones,
    • Question 33: Electron transport chain location:

      • Occurs in the inner membrane of the mitochondria
    • Question 34: Membrane transport for phagosome formation:

      • Receptor-mediated endocytosis.
    • Question 35: Interphase DNA shortening into metaphase chromosomes:

      • Occurs approximately 100-1000 times during cell division.
    • Question 36: Aerobic respiration compound:

      • Oxygen is necessary for aerobic respiration
    • Question 37: Histone H1 and DNA condensation process:

      • Histone H1 plays a role in chromatin organization, specifically the formation of the 30 nm fiber.
    • Question 38: Ligands transported through circulatory system:

      • Neurotransmitters and hormones, including adrenaline (epinephrine).
    • Question 39: Phospholipid movements by enzymes:

      • Enzymes catalyze transverse "flip-flop" movements of phospholipids within the membrane.
    • Question 40: Definition of a codon:

      • Three nucleotides of DNA constitute a codon.
    • Question 41: START codon recognition in eukaryotes:

      • Small ribosomal subunit, initiator tRNA, and mRNA interact to recognize the START codon.
    • Question 42: Glycolipid component:

      • Sphingosine is a component of a glycolipid
    • Question 43: DNA strands and DNA duplex formation:

      • Specific sequences like 5'-AAGCCGTT-3' can form a DNA duplex by pairing with themselves if the sequence is palindrome
    • Question 44: Not applicable. This is not a question, but a list of words or terms.

    • Question 45: Not applicable.

    • Question 46: Not applicable.

    • Question 47: Not applicable.

    • Question 48: Not applicable.

    • Question 49: Not applicable.

    • Question 50: Not applicable.

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