Molecular Biology Calculations and Recombinant Clones Quiz

Look for clones that have incorporated the desired DNA sequence. This can be done through PCR or DNA sequencing. The clone should contain the correct DNA fragment before further analysis. Selecting recombinant clones involves identifying bacteria that have successfully incorporated a plasmid with a gene of interest, achieved using antibiotic resistance markers.

The principle is to use antibiotic resistance markers. For example, if the plasmid confers ampicillin resistance (Ampr), only E. coli cells that have taken up this plasmid will grow on media containing ampicillin. This ensures that only genetically transformed cells are studied further.

It indicates viable cells and successful transformation. However, it does not confirm the presence of the specific gene of interest (GOI)-containing plasmid. Further steps are required to isolate and confirm the presence of the specific GOI-containing plasmid.

<p>It is significant in molecular cloning to ensure that only genetically transformed cells are studied further. This is achieved using antibiotic resistance markers, where only cells that have taken up the plasmid will grow on media containing the corresponding antibiotic.</p> Signup and view all the answers

<p>Potential methods include PCR or DNA sequencing to confirm the presence of the desired DNA sequence in the recombinant clones before further analysis.</p> Signup and view all the answers