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Molecular Biology and Diagnostics Quiz

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Questions and Answers

What is the analyte for molecular testing in contrast to the analysis of phenotypic traits?

Carbohydrate fermentation patterns

Genome, transcriptome, or proteome of the microorganism (correct)

Enzyme or pigment production

Microscopic and colonial morphologies

What is the purpose of molecular epidemiology in outbreak situations?

To determine the colony morphology

To compare antibiotic resistance patterns

To ascertain whether the isolates have a common or independent source (correct)

To identify individual genetic traits of the microorganisms

Why are some microorganisms targeted by molecular-based laboratory tests?

They are difficult and/or time-consuming to isolate (correct)

They are resistant to molecular testing

They have high susceptibility to antibiotics

They are easily cultured using traditional methods

What is the focus of nucleic acid amplification methods in detecting antimicrobial resistance?

<p>$Genes$ involved in the emergence of antimicrobial resistance</p> Signup and view all the answers

11.3

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Study Notes

Molecular Testing

The analyte for molecular testing is the genetic material (DNA or RNA) of an organism, in contrast to the analysis of phenotypic traits which are the physical and behavioral characteristics of an organism.

Molecular Epidemiology

The purpose of molecular epidemiology in outbreak situations is to identify the source and track the spread of an infectious disease by analyzing the genetic material of the causative agent.

Targeted Microorganisms

Some microorganisms are targeted by molecular-based laboratory tests because they are difficult to culture, require specialized growth conditions, or have a fastidious nature, making it hard to detect them using traditional methods.

Nucleic Acid Amplification

The focus of nucleic acid amplification methods in detecting antimicrobial resistance is to identify specific genetic mutations associated with resistance to certain antibiotics, allowing for targeted treatment and effective infection control.

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Description

Test your knowledge of detection and identification of microorganisms using molecular-based laboratory tests. This quiz covers the common targets for molecular-based tests, advantages and disadvantages of molecular-based methods compared to traditional culture-based methods, and differentiation between organisms for which nucleic acid-based tests are available.