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Molecular Biology and Diagnostics Quiz
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Molecular Biology and Diagnostics Quiz

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@DauntlessSphene

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Questions and Answers

What is the analyte for molecular testing in contrast to the analysis of phenotypic traits?

  • Carbohydrate fermentation patterns
  • Genome, transcriptome, or proteome of the microorganism (correct)
  • Enzyme or pigment production
  • Microscopic and colonial morphologies
  • What is the purpose of molecular epidemiology in outbreak situations?

  • To determine the colony morphology
  • To compare antibiotic resistance patterns
  • To ascertain whether the isolates have a common or independent source (correct)
  • To identify individual genetic traits of the microorganisms
  • Why are some microorganisms targeted by molecular-based laboratory tests?

  • They are difficult and/or time-consuming to isolate (correct)
  • They are resistant to molecular testing
  • They have high susceptibility to antibiotics
  • They are easily cultured using traditional methods
  • What is the focus of nucleic acid amplification methods in detecting antimicrobial resistance?

    <p>$Genes$ involved in the emergence of antimicrobial resistance</p> Signup and view all the answers

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    Study Notes

    Molecular Testing

    • The analyte for molecular testing is the genetic material (DNA or RNA) of an organism, in contrast to the analysis of phenotypic traits which are the physical and behavioral characteristics of an organism.

    Molecular Epidemiology

    • The purpose of molecular epidemiology in outbreak situations is to identify the source and track the spread of an infectious disease by analyzing the genetic material of the causative agent.

    Targeted Microorganisms

    • Some microorganisms are targeted by molecular-based laboratory tests because they are difficult to culture, require specialized growth conditions, or have a fastidious nature, making it hard to detect them using traditional methods.

    Nucleic Acid Amplification

    • The focus of nucleic acid amplification methods in detecting antimicrobial resistance is to identify specific genetic mutations associated with resistance to certain antibiotics, allowing for targeted treatment and effective infection control.

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    Description

    Test your knowledge of detection and identification of microorganisms using molecular-based laboratory tests. This quiz covers the common targets for molecular-based tests, advantages and disadvantages of molecular-based methods compared to traditional culture-based methods, and differentiation between organisms for which nucleic acid-based tests are available.

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