8 Questions
What is considered the minimum inhibitory concentration (MIC) of an antimicrobial agent against a bacterium?
The lowest concentration that prevents bacterial growth
What is the purpose of adjusting the bacterial suspension to achieve turbidity equivalent to that of 0.5 McFarland standard?
To ensure consistent inoculum density
What is the optimal time frame for using adjusted suspensions for final inoculation after preparation?
Within 15 minutes of preparation
What is the recommended temperature range for solidifying agar dilution plates after pouring the agar and antimicrobial solution?
45 to 50°C
Why are Petri dishes with grids usually used when preparing agar dilution plates?
To assist in counting colonies
What is the purpose of preparing serial two-fold dilutions of an antimicrobial agent?
To test various concentrations against bacteria
What is the significance of achieving turbidity equivalent to that of 0.5 McFarland standard in bacterial suspensions?
It ensures uniform density for testing purposes
Why is it important to pour agar and antimicrobial solution into Petri dishes on a level surface?
To ensure even distribution within the plate
Learn about the minimum inhibitory concentration (MIC) and bacterial growth assessment through turbidity examination. Understand how MIC helps determine the lowest antibiotic concentration inhibiting bacterial growth.
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