Minimum Inhibitory Concentration and Bacterial Growth
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Questions and Answers

What is considered the minimum inhibitory concentration (MIC) of an antimicrobial agent against a bacterium?

  • The concentration that kills all bacteria
  • The highest concentration that promotes bacterial growth
  • The lowest concentration that prevents bacterial growth (correct)
  • The average concentration of antimicrobial agents

    • What is the purpose of adjusting the bacterial suspension to achieve turbidity equivalent to that of 0.5 McFarland standard?

  • To ensure consistent inoculum density (correct)
  • To prevent bacterial growth
  • To reduce the effectiveness of antimicrobial agents
  • To enhance bacterial growth

    • What is the optimal time frame for using adjusted suspensions for final inoculation after preparation?

  • Within 1 hour of preparation
  • Within 30 minutes of preparation
  • Within 15 minutes of preparation (correct)
  • Within 2 hours of preparation

    • What is the recommended temperature range for solidifying agar dilution plates after pouring the agar and antimicrobial solution?

    <p>45 to 50°C</p> Signup and view all the answers

    Why are Petri dishes with grids usually used when preparing agar dilution plates?

    <p>To assist in counting colonies</p> Signup and view all the answers

    What is the purpose of preparing serial two-fold dilutions of an antimicrobial agent?

    <p>To test various concentrations against bacteria</p> Signup and view all the answers

    What is the significance of achieving turbidity equivalent to that of 0.5 McFarland standard in bacterial suspensions?

    <p>It ensures uniform density for testing purposes</p> Signup and view all the answers

    Why is it important to pour agar and antimicrobial solution into Petri dishes on a level surface?

    <p>To ensure even distribution within the plate</p> Signup and view all the answers

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