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Questions and Answers
What does the agar thickness affect in the agar dilution method?
What does the agar thickness affect in the agar dilution method?
- Microbial growth
- Antibiotic concentration
- Incubation time
- Inhibition zone size (correct)
What does a spot on the agar surface after incubation indicate?
What does a spot on the agar surface after incubation indicate?
- High antibiotic concentration
- Low bacterial count
- No inhibition
- Growth of bacteria (correct)
What does a large inhibition zone in the disk diffusion method suggest?
What does a large inhibition zone in the disk diffusion method suggest?
- Inadequate antibiotic concentration
- Resistant bacteria
- Susceptible bacteria (correct)
- No bacteria present
What is one advantage of the agar dilution method over broth mediums?
What is one advantage of the agar dilution method over broth mediums?
Why is the disk diffusion method also known as the Kirby Bauer method?
Why is the disk diffusion method also known as the Kirby Bauer method?
What problem is associated with agar that is too thick in the agar dilution method?
What problem is associated with agar that is too thick in the agar dilution method?
Why are media supplements or different media required to obtain good growth and reliable susceptibility profiles for bacteria?
Why are media supplements or different media required to obtain good growth and reliable susceptibility profiles for bacteria?
Which organization is responsible for publishing and updating standards related to susceptibility testing methods?
Which organization is responsible for publishing and updating standards related to susceptibility testing methods?
What is the purpose of the growth control in the broth dilution method procedure?
What is the purpose of the growth control in the broth dilution method procedure?
Why are susceptibility tests not performed on bacteria that are predictably susceptible to commonly used antimicrobial agents?
Why are susceptibility tests not performed on bacteria that are predictably susceptible to commonly used antimicrobial agents?
Why is it important for clinical laboratories to be aware of the antimicrobial agents appropriate for testing?
Why is it important for clinical laboratories to be aware of the antimicrobial agents appropriate for testing?
When is it necessary to perform antimicrobial susceptibility testing on bacteria according to the text?
When is it necessary to perform antimicrobial susceptibility testing on bacteria according to the text?
What is the significance of maintaining awareness of the reliability of different test systems for detecting antimicrobial resistance?
What is the significance of maintaining awareness of the reliability of different test systems for detecting antimicrobial resistance?
Why would Escherichia coli isolated from stool not undergo susceptibility testing?
Why would Escherichia coli isolated from stool not undergo susceptibility testing?
In the broth dilution method, what is the purpose of the sterility control?
In the broth dilution method, what is the purpose of the sterility control?
Which factor is NOT considered when determining whether antimicrobial susceptibility testing is warranted?
Which factor is NOT considered when determining whether antimicrobial susceptibility testing is warranted?
In which scenario would viridans group streptococci not undergo routine susceptibility testing?
In which scenario would viridans group streptococci not undergo routine susceptibility testing?
Why would coagulase-negative staphylococci isolated from multiple blood cultures be subjected to susceptibility testing?
Why would coagulase-negative staphylococci isolated from multiple blood cultures be subjected to susceptibility testing?
What is the primary advantage of breakpoint panels?
What is the primary advantage of breakpoint panels?
How is an MIC test performed using an agar-dilution method?
How is an MIC test performed using an agar-dilution method?
What happens if the lactamase test result is negative?
What happens if the lactamase test result is negative?
Why is MUELLER-HINTON AGAR recommended for testing aerobic isolates?
Why is MUELLER-HINTON AGAR recommended for testing aerobic isolates?
What is the primary disadvantage of breakpoint panels?
What is the primary disadvantage of breakpoint panels?
What is the purpose of spot-inoculating test bacteria onto agar plates in an MIC test?
What is the purpose of spot-inoculating test bacteria onto agar plates in an MIC test?
What should be done if there is doubt about the validity of the results in the broth microdilution method?
What should be done if there is doubt about the validity of the results in the broth microdilution method?
What is a shortcoming of the broth microdilution MIC method mentioned in the text?
What is a shortcoming of the broth microdilution MIC method mentioned in the text?
What does 'breakpoint' refer to in the context of antimicrobial agents?
What does 'breakpoint' refer to in the context of antimicrobial agents?
What does it indicate if there is no growth in either well when two concentrations are tested?
What does it indicate if there is no growth in either well when two concentrations are tested?
When is an isolate considered resistant based on its penicillin MIC value?
When is an isolate considered resistant based on its penicillin MIC value?
What should be done with penicillin MICs of $0.06$ to $0.12$ μɡ/mL in relation to an induced β-lactamase test?
What should be done with penicillin MICs of $0.06$ to $0.12$ μɡ/mL in relation to an induced β-lactamase test?
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