Mini GC Experiment Procedures

<p>To compare the unknown mixture's data to the known substances' data for identification. (C)</p> Signup and view all the answers

<p>To identify the individual components of a mixture based on their volatility. (D)</p> Signup and view all the answers

<p>0.10 μL (D)</p> Signup and view all the answers

<p>Rotate it slightly while inserting. (A)</p> Signup and view all the answers

<p>More than three flushes (D)</p> Signup and view all the answers

<p>The syringe plunger moves smoothly. (C)</p> Signup and view all the answers

<p>Depress the syringe plunger. (C)</p> Signup and view all the answers

<p>Wipe the needle and prepare for injection. (A)</p> Signup and view all the answers

<p>Replace it with the compound's name. (A)</p> Signup and view all the answers

<p>New Experiment (C)</p> Signup and view all the answers

<p>To prevent contamination of the known substances (D)</p> Signup and view all the answers

<p>65°C (D)</p> Signup and view all the answers

<p>It could damage the syringe and affect measurements (A)</p> Signup and view all the answers

<p>To identify substances in a mixture based on retention times (D)</p> Signup and view all the answers

<p>4.0 kPa (B)</p> Signup and view all the answers

<p>It interacts with the compounds to separate them (D)</p> Signup and view all the answers

<p>To ensure accurate data collection (B)</p> Signup and view all the answers

<p>Dispose in the Non-halogenated Organic Waste Container (A)</p> Signup and view all the answers

<p>Fewer intermolecular interactions with the stationary phase (D)</p> Signup and view all the answers

<p>10.0 min (D)</p> Signup and view all the answers

<p>Atmospheric air (C)</p> Signup and view all the answers

<p>Stronger intermolecular interactions result in longer retention times (C)</p> Signup and view all the answers

<p>35°C (B)</p> Signup and view all the answers

<p>Performing drug tests and toxin screens (C)</p> Signup and view all the answers

<p>The nonpolar coating of the stationary phase (B)</p> Signup and view all the answers

<p>Gas chromatography (C)</p> Signup and view all the answers

<p>The specific time a compound takes to exit the column after injection (C)</p> Signup and view all the answers

<p>Molecular shape of the compound (C)</p> Signup and view all the answers

<p>They move through the column more rapidly (B)</p> Signup and view all the answers

<p>To produce electrical responses proportional to the concentration of the compounds (B)</p> Signup and view all the answers

<p>Ethanol (D)</p> Signup and view all the answers

<p>Avoid flames and do not inhale or swallow any chemicals (C)</p> Signup and view all the answers

<p>To identify the specific substances present in a mixture (D)</p> Signup and view all the answers

<p>Ethanol (A)</p> Signup and view all the answers

The unique time for a compound to exit the chromatography column.

A technique for identifying compounds in a mixture based on their retention times.

The nonpolar coating that retains solutes of similar polarity in chromatography.

The phase that carries compounds through the chromatography column.

Compounds with lower boiling points that move quickly through the column.

Interactions that can affect retention time, especially in polar compounds.

A factor that may slightly influence the retention time in chromatography.

A visual output showing the presence of compounds by peaks.

Wash hands with soap and water before lab exit.

Use Non-halogenated Organic Waste Container for chemical disposal.

Prepare Vernier Mini GC for data collection: temperature and time settings.

Set the initial temperature to 35°C for gas chromatography.

Final temperature for the GC process is set to 65°C.

Set pressure to 4.0 kPa for the GC operation.

Clean glass syringe with methanol; fill, expel, and repeat.

Handle the glass syringe gently to avoid damage.

A technique used to separate and analyze compounds in gaseous mixtures.

Forces that determine how compounds adhere to the stationary phase.

An apparatus used in the lab for gas chromatography experiments.

The tendency of a substance to interact with the stationary phase.

The temperature-pressure setting specific for the Mini Gas Chromatograph during analysis.

A sample with uncharacterized components to be identified using chromatography.

The process of calculating the area under peaks on a chromatogram to quantify components.

The action of storing test results in the Vernier Instrumental Analysis program for later use.

A solvent used in gas chromatography for sample injection.

The process of flushing the syringe to remove previous samples before use.

The entry point on the Mini GC where the sample is injected.

The moment data analysis begins after injecting the sample.

Selecting and quantifying a peak on the chromatogram for analysis.

The time a compound takes to pass through the detector in chromatography.

Settings for the Mini GC that must be replicated for each run.

A special wipe used to clean the syringe needle carefully.