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Microtomy and Block Trimming Quiz
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Microtomy and Block Trimming Quiz

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Questions and Answers

What is the recommended temperature for the water bath during section cutting?

  • 10 degrees Celsius lower than the melting point of paraffin (correct)
  • 5 degrees Celsius lower than the melting point of paraffin
  • Equal to the melting point of paraffin
  • 5 degrees Celsius higher than the melting point of paraffin
  • Who is credited with the discovery of the Rocking Microtome?

  • Kimberly Enclonar
  • No specific individual is credited
  • Paldwell Trefall (correct)
  • Gabriel Ponce de Leon
  • What is the recommended thickness for cutting sections using the Rocking Microtome?

  • 2-4um
  • 4-6um
  • 8-10um
  • 10-12um (correct)
  • What is the purpose of block trimming in microtomy?

    <p>To create parallel sides for better grip during tissue sectioning</p> Signup and view all the answers

    What type of microtome is not recommended for serial sections?

    <p>Rocking Microtome</p> Signup and view all the answers

    Study Notes

    Microtomy Essentials

    • The recommended temperature for the water bath during section cutting is around 40-50°C.

    History of Microtome

    • The discovery of the Rocking Microtome is credited to H. von Peham.

    Section Cutting

    • The recommended thickness for cutting sections using the Rocking Microtome is 1-2 μm.

    Block Trimming

    • The purpose of block trimming in microtomy is to remove excess embedding medium and to shape the block to fit the microtome.

    Microtome Types

    • Rotary microtomes are not recommended for serial sections.

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    Description

    Test your knowledge of microtomy and block trimming with this quiz. Explore the process of creating uniform tissue sections and the use of spring-balanced teeth and ratchet feed wheels. Perfect for students of histology and laboratory professionals.

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