Podcast
Questions and Answers
What is the recommended temperature for the water bath during section cutting?
What is the recommended temperature for the water bath during section cutting?
- 10 degrees Celsius lower than the melting point of paraffin (correct)
- 5 degrees Celsius lower than the melting point of paraffin
- Equal to the melting point of paraffin
- 5 degrees Celsius higher than the melting point of paraffin
Who is credited with the discovery of the Rocking Microtome?
Who is credited with the discovery of the Rocking Microtome?
- Kimberly Enclonar
- No specific individual is credited
- Paldwell Trefall (correct)
- Gabriel Ponce de Leon
What is the recommended thickness for cutting sections using the Rocking Microtome?
What is the recommended thickness for cutting sections using the Rocking Microtome?
- 2-4um
- 4-6um
- 8-10um
- 10-12um (correct)
What is the purpose of block trimming in microtomy?
What is the purpose of block trimming in microtomy?
What type of microtome is not recommended for serial sections?
What type of microtome is not recommended for serial sections?
Flashcards
Water bath temperature
Water bath temperature
10 degrees Celsius lower than the melting point of paraffin.
Rocking Microtome inventor
Rocking Microtome inventor
Paldwell Trefall.
Rocking Microtome section thickness
Rocking Microtome section thickness
10-12um
Block Trimming purpose
Block Trimming purpose
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Microtome for serial sections
Microtome for serial sections
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Study Notes
Microtomy Essentials
- The recommended temperature for the water bath during section cutting is around 40-50°C.
History of Microtome
- The discovery of the Rocking Microtome is credited to H. von Peham.
Section Cutting
- The recommended thickness for cutting sections using the Rocking Microtome is 1-2 μm.
Block Trimming
- The purpose of block trimming in microtomy is to remove excess embedding medium and to shape the block to fit the microtome.
Microtome Types
- Rotary microtomes are not recommended for serial sections.
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Description
Test your knowledge of microtomy and block trimming with this quiz. Explore the process of creating uniform tissue sections and the use of spring-balanced teeth and ratchet feed wheels. Perfect for students of histology and laboratory professionals.
