Podcast
Questions and Answers
What could cause crooked or uneven ribbons when sectioning?
What could cause crooked or uneven ribbons when sectioning?
- Blockholder misalignment
- Excessive dehydration of the block
- Improper adjustment of the blockface
- Dull knife edge (correct)
Which factor is most likely to contribute to holes in the sections?
Which factor is most likely to contribute to holes in the sections?
- Excessive dehydration during processing (correct)
- Dirty knife edge
- Non-parallel knife and block edges
- Improper cooling of the block
What is a likely cause of sections that will not ribbon during microtomy?
What is a likely cause of sections that will not ribbon during microtomy?
- Uneven chilling of the block
- Improper knife clearance angle (correct)
- Impurities in the wax
- Blockface unevenly sectioned
Which scenario could lead to lifting of the section from the blade as the block is raised?
Which scenario could lead to lifting of the section from the blade as the block is raised?
What might result from a blockholder not being adjusted to parallel the blade?
What might result from a blockholder not being adjusted to parallel the blade?
What is a potential cause for the washboarding effect observed in tissue sections?
What is a potential cause for the washboarding effect observed in tissue sections?
Which factor could lead to chatter or microscopic vibration during sectioning?
Which factor could lead to chatter or microscopic vibration during sectioning?
What issue is likely caused by a blunt or dirty knife during tissue sectioning?
What issue is likely caused by a blunt or dirty knife during tissue sectioning?
Which condition is a likely cause of uneven section thickness?
Which condition is a likely cause of uneven section thickness?
What common factor may contribute to the phenomenon of warm tissue blocks complicating sectioning?
What common factor may contribute to the phenomenon of warm tissue blocks complicating sectioning?
Which practice is likely to produce scratch lines in tissue sections?
Which practice is likely to produce scratch lines in tissue sections?
What is the effect of insufficient embedding wax on tissue section quality?
What is the effect of insufficient embedding wax on tissue section quality?
What is a frequent consequence of using a worn microtome blade?
What is a frequent consequence of using a worn microtome blade?
Flashcards
Uneven ribbons
Uneven ribbons
Microtome ribbons that are not uniform in thickness or shape.
Uneven blockface
Uneven blockface
The surface of the tissue block is not uniformly sectioned.
Holes in sections
Holes in sections
Sections with gaps or holes. Indicates aggressive blockfacing.
Sections won't ribbon
Sections won't ribbon
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Section lifting
Section lifting
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Washboarding in sections
Washboarding in sections
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Chatter in sections
Chatter in sections
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Uneven section thickness
Uneven section thickness
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Wrinkled tissue sections
Wrinkled tissue sections
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Vertical Section Splitting
Vertical Section Splitting
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Microtome Blade Dullness
Microtome Blade Dullness
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Inappropriate Clearance Angle
Inappropriate Clearance Angle
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Insufficient Embedding Wax
Insufficient Embedding Wax
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