Microtomy Techniques Quiz

<p>Too fine knife clearance angle (B)</p> Signup and view all the answers

<p>Blockface unevenly sectioned (B)</p> Signup and view all the answers

<p>Overfixed tissue (A)</p> Signup and view all the answers

<p>Cutting with excessive speed (C)</p> Signup and view all the answers

<p>Wrinkled, compressed, or jammed sections (A)</p> Signup and view all the answers

<p>Loose knife or block (C)</p> Signup and view all the answers

<p>Improper cutting speed (C)</p> Signup and view all the answers

<p>Utilizing a worn microtome (D)</p> Signup and view all the answers

<p>Irregularities in the sections (C)</p> Signup and view all the answers

<p>Uneven sectioning (A)</p> Signup and view all the answers

Microtome ribbons that are not uniform in thickness or shape.

The surface of the tissue block is not uniformly sectioned.

Sections with gaps or holes. Indicates aggressive blockfacing.

Microtome sections failing to form continuous ribbons.

Microtome sections detaching from the blade as the block is moved.

Undulations or a wavy appearance in tissue sections.

Microscopic vibrations in tissue sections, often uneven.

Sections that vary significantly in thickness (thick/thin/skipped).

Sections that are deformed or compressed due to improper cutting.

Tissue sections that split vertically during sectioning

A dull microtome blade causes uneven tissue sections.

Incorrect angle between the microtome blade and the tissue block.

Not enough embedding wax during tissue processing impacts section quality.