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13 Questions

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Microtomy Techniques Quiz

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Questions and Answers

What could cause crooked or uneven ribbons when sectioning?

Blockholder misalignment

Excessive dehydration of the block

Improper adjustment of the blockface

Dull knife edge (correct)

Which factor is most likely to contribute to holes in the sections?

Excessive dehydration during processing (correct)

Dirty knife edge

Non-parallel knife and block edges

Improper cooling of the block

What is a likely cause of sections that will not ribbon during microtomy?

Uneven chilling of the block

Improper knife clearance angle (correct)

Impurities in the wax

Blockface unevenly sectioned

Which scenario could lead to lifting of the section from the blade as the block is raised?

Too fine knife clearance angle Signup and view all the answers

What might result from a blockholder not being adjusted to parallel the blade?

Blockface unevenly sectioned Signup and view all the answers

What is a potential cause for the washboarding effect observed in tissue sections?

Overfixed tissue Signup and view all the answers

Which factor could lead to chatter or microscopic vibration during sectioning?

Cutting with excessive speed Signup and view all the answers

What issue is likely caused by a blunt or dirty knife during tissue sectioning?

Wrinkled, compressed, or jammed sections Signup and view all the answers

Which condition is a likely cause of uneven section thickness?

Loose knife or block Signup and view all the answers

What common factor may contribute to the phenomenon of warm tissue blocks complicating sectioning?

Improper cutting speed Signup and view all the answers

Which practice is likely to produce scratch lines in tissue sections?

Utilizing a worn microtome Signup and view all the answers

What is the effect of insufficient embedding wax on tissue section quality?

Irregularities in the sections Signup and view all the answers

What is a frequent consequence of using a worn microtome blade?

Uneven sectioning Signup and view all the answers