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Questions and Answers
What is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness?
What is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness?
- Pawl
- Spring-balanced teeth
- Block holder
- Ratchet feed wheel connected to a micrometer screw (correct)
What is the recommended thickness for cutting sections during section cutting?
What is the recommended thickness for cutting sections during section cutting?
- 4-6um (correct)
- 2-4um
- 6-8um
- 8-10um
What is the recommended angulation for tilting the knife during section cutting?
What is the recommended angulation for tilting the knife during section cutting?
- 30-45 degrees
- 45-60 degrees
- 0-15 degrees (correct)
- 15-30 degrees
What is the recommended temperature for the water bath during section cutting?
What is the recommended temperature for the water bath during section cutting?
Who is credited with the discovery of the Rocking Microtome?
Who is credited with the discovery of the Rocking Microtome?
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Study Notes
Microtome Components and Techniques
- Micrometer: This mechanism is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness.
- Section Thickness: 4-6 micrometers is the recommended thickness for cutting sections during section cutting.
- Knife Angulation: A 10-15° angle is recommended for tilting the knife during section cutting.
- Water Bath Temperature: The water bath should be maintained at 40-45°C during section cutting
- Rocking Microtome: Introduced by Minot in the late 19th century, the Rocking Microtome revolutionized section cutting by providing a precise and consistent method for producing thin tissue sections suitable for microscopic examination.
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