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Microtomy and Block Trimming Quiz

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Questions and Answers

What is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness?

Pawl

Spring-balanced teeth

Block holder

Ratchet feed wheel connected to a micrometer screw (correct)

What is the recommended thickness for cutting sections during section cutting?

4-6um (correct)

2-4um

6-8um

8-10um

What is the recommended angulation for tilting the knife during section cutting?

30-45 degrees

45-60 degrees

0-15 degrees (correct)

15-30 degrees

What is the recommended temperature for the water bath during section cutting?

<p>10 degrees Celsius lower than the melting point of paraffin (B)</p> Signup and view all the answers

Who is credited with the discovery of the Rocking Microtome?

<p>Paldwell Trefall (A)</p> Signup and view all the answers

Study Notes

Microtome Components and Techniques

Micrometer: This mechanism is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness.
Section Thickness: 4-6 micrometers is the recommended thickness for cutting sections during section cutting.
Knife Angulation: A 10-15° angle is recommended for tilting the knife during section cutting.
Water Bath Temperature: The water bath should be maintained at 40-45°C during section cutting
Rocking Microtome: Introduced by Minot in the late 19th century, the Rocking Microtome revolutionized section cutting by providing a precise and consistent method for producing thin tissue sections suitable for microscopic examination.

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Description

Test your knowledge of microtomy and block trimming with this quiz. Explore the process of creating uniform tissue sections and the use of spring-balanced teeth and ratchet feed wheels. Perfect for students of histology and laboratory professionals.