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Questions and Answers
What is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness?
What is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness?
- Pawl
- Spring-balanced teeth
- Block holder
- Ratchet feed wheel connected to a micrometer screw (correct)
What is the recommended thickness for cutting sections during section cutting?
What is the recommended thickness for cutting sections during section cutting?
- 4-6um (correct)
- 2-4um
- 6-8um
- 8-10um
What is the recommended angulation for tilting the knife during section cutting?
What is the recommended angulation for tilting the knife during section cutting?
- 30-45 degrees
- 45-60 degrees
- 0-15 degrees (correct)
- 15-30 degrees
What is the recommended temperature for the water bath during section cutting?
What is the recommended temperature for the water bath during section cutting?
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Who is credited with the discovery of the Rocking Microtome?
Who is credited with the discovery of the Rocking Microtome?
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Study Notes
Microtome Components and Techniques
- Micrometer: This mechanism is used to move the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness.
- Section Thickness: 4-6 micrometers is the recommended thickness for cutting sections during section cutting.
- Knife Angulation: A 10-15° angle is recommended for tilting the knife during section cutting.
- Water Bath Temperature: The water bath should be maintained at 40-45°C during section cutting
- Rocking Microtome: Introduced by Minot in the late 19th century, the Rocking Microtome revolutionized section cutting by providing a precise and consistent method for producing thin tissue sections suitable for microscopic examination.
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