Recent Lessons

Show all results for ""

Feature Overview

Ace your exams with our all-in-one platform for creating and sharing quizzes and tests.

Quizzes

Create quizzes and tests automatically from your content using AI.

Flashcards

Automatically turn your notes into digital flashcards.

Share, Export & Embed

Share with classmates or export to Excel and your learning management system.

Stats & Reporting

Auto-grading quizzes and tests with detailed stats and reports.

Mobile Apps

The smarter way to study – wherever you are.

Pricing

Search...

9 Questions

7 Views

Microscopy Techniques: TEM vs SEM

Choose a study mode

Play Quiz

Study Flashcards

Spaced Repetition

Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

0

False

0

False

1

False

2

False Signup and view all the answers

3

False Signup and view all the answers

What is the main purpose of a Transmission Electron Microscope (TEM)?

To study the internal ultrastructure of cells Signup and view all the answers

How is the image in a Transmission Electron Microscope (TEM) enhanced?

By staining the thin section of preserved cells with atoms of heavy metals Signup and view all the answers

What is the main difference between a Transmission Electron Microscope (TEM) and a Scanning Electron Microscope (SEM)?

TEM studies the surface structure, while SEM studies the internal ultrastructure of cells Signup and view all the answers

How does a Scanning Electron Microscope (SEM) produce its images?

By collecting and focusing secondary electrons from the sample surface Signup and view all the answers

Study Notes

Transmission Electron Microscope (TEM)

The primary purpose of a Transmission Electron Microscope (TEM) is to provide high-resolution images of the internal structure of specimens by transmitting electrons through the sample.
TEM achieves image enhancement through contrast mechanisms, including phase contrast and absorption contrast, often aided by metal staining to increase electron density in certain areas.

Scanning Electron Microscope (SEM)

The main distinction between TEM and Scanning Electron Microscope (SEM) is that SEM primarily scans the surface of samples to create three-dimensional images, while TEM focuses on internal structures.
SEM generates images by directing a focused beam of electrons onto the specimen's surface, detecting secondary electrons emitted from the surface to construct detailed topographic images.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Learn about the differences between Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM) and their applications in studying cell structures. Understand how TEM is used to study internal ultrastructure of cells through thin sections, while SEM is used to study surface structure of cells with a thin film of gold.