Microscopy Techniques: TEM vs SEM

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False (B)

False (B)

False (B)

False (B) Signup and view all the answers

False (B) Signup and view all the answers

To study the internal ultrastructure of cells (B) Signup and view all the answers

By staining the thin section of preserved cells with atoms of heavy metals (D) Signup and view all the answers

TEM studies the surface structure, while SEM studies the internal ultrastructure of cells (B) Signup and view all the answers

By collecting and focusing secondary electrons from the sample surface (A) Signup and view all the answers

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The primary purpose of a Transmission Electron Microscope (TEM) is to provide high-resolution images of the internal structure of specimens by transmitting electrons through the sample.
TEM achieves image enhancement through contrast mechanisms, including phase contrast and absorption contrast, often aided by metal staining to increase electron density in certain areas.

The main distinction between TEM and Scanning Electron Microscope (SEM) is that SEM primarily scans the surface of samples to create three-dimensional images, while TEM focuses on internal structures.
SEM generates images by directing a focused beam of electrons onto the specimen's surface, detecting secondary electrons emitted from the surface to construct detailed topographic images.