Microbiology and Electron Microscopy Quiz
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Questions and Answers

What type of microscope uses a beam of electrons to produce an image?

  • Stereo microscope
  • Confocal microscope
  • Light microscope
  • Electron microscope (correct)
  • Staining improves the visibility of living cells under bright field illumination.

    False (B)

    What are photographs taken using electron microscopy called?

    Electron micrographs

    The maximum magnification level of an electron microscope can reach up to _____ times.

    <p>2 X 10^6</p> Signup and view all the answers

    Which type of electron microscope is used to view extremely thin sections of material?

    <p>Transmission electron microscope (C)</p> Signup and view all the answers

    Match the following types of electron microscopes with their functions:

    <p>Scanning electron microscope = Produces detailed images of surface structures Transmission electron microscope = Reveals internal details of a structure Electron micrographs = Images obtained from electron microscopy Magnification = Enhancement of image size</p> Signup and view all the answers

    Electrons have longer wavelengths than light particles.

    <p>False (B)</p> Signup and view all the answers

    Why must specimens be prepared before viewing under an electron microscope?

    <p>Specimens must be dead.</p> Signup and view all the answers

    What is the primary function of the glycocalyx in bacteria?

    <p>Protection and attachment (D)</p> Signup and view all the answers

    A capsule is a loosely attached glycocalyx layer.

    <p>False (B)</p> Signup and view all the answers

    Name the type of bacteria that has a thick cell wall and is illustrated as Bacillus megaterium.

    <p>Bacillus megaterium</p> Signup and view all the answers

    Bacteria that possess a single polar flagellum are referred to as __________.

    <p>monotrichous</p> Signup and view all the answers

    Match the following bacterial structures with their functions:

    <p>Flagella = Locomotion Capsule = Protection from immune system Fimbriae = Attachment to surfaces Glycocalyx = Viscous outer layer</p> Signup and view all the answers

    Which bacterium is associated with anthrax due to its encapsulated structure?

    <p>Bacillus anthracis (D)</p> Signup and view all the answers

    Fimbriae are longer and thicker than flagella.

    <p>False (B)</p> Signup and view all the answers

    Describe the role of a glycocalyx in pathogenic bacteria.

    <p>It protects against the host's immune system.</p> Signup and view all the answers

    Which of the following is NOT one of the five classes of biological molecules?

    <p>Minerals (A)</p> Signup and view all the answers

    The molecular formula provides information about the structure of a molecule.

    <p>False (B)</p> Signup and view all the answers

    What type of biological molecules are primarily composed of carbon, hydrogen, oxygen, nitrogen, and sulfur?

    <p>Organic compounds</p> Signup and view all the answers

    The molecular formula for glucose is C6H12O6, while its structural formula can be represented in several forms including ______ and ______.

    <p>ring form, straight form</p> Signup and view all the answers

    Match the biological molecules with their primary components:

    <p>Proteins = Amino acids Carbohydrates = Sugar molecules Nucleic acids = Nucleotides Lipids = Fatty acids</p> Signup and view all the answers

    Which of the following models represents the structural formula of a molecule?

    <p>Ball and stick model (B)</p> Signup and view all the answers

    Organic compounds always contain carbon, oxygen, and nitrogen.

    <p>False (B)</p> Signup and view all the answers

    Can you name any two elements commonly found in organic molecules?

    <p>Hydrogen, Oxygen</p> Signup and view all the answers

    What is formed when sodium and chlorine atoms bond together?

    <p>Sodium chloride (A)</p> Signup and view all the answers

    Covalent bonds are created by the transfer of electrons between atoms.

    <p>False (B)</p> Signup and view all the answers

    What type of bond is formed when an atom loses an electron?

    <p>Cation</p> Signup and view all the answers

    An ion with a negative charge is called a ______.

    <p>anion</p> Signup and view all the answers

    Match the terms with their corresponding definitions:

    <p>Cation = Ion with a positive charge Anion = Ion with a negative charge Ionic bond = Bond formed through electrostatic attraction Covalent bond = Bond formed by sharing electron pairs</p> Signup and view all the answers

    Which type of bond is directional and involves the sharing of electron pairs?

    <p>Covalent bond (D)</p> Signup and view all the answers

    Ionic bonds result from the attraction between two atoms of the same charge.

    <p>False (B)</p> Signup and view all the answers

    What do you call a bond formed when two pairs of electrons are shared?

    <p>Double covalent bond</p> Signup and view all the answers

    What is the main product of a condensation reaction between two monosaccharides?

    <p>Disaccharide (B)</p> Signup and view all the answers

    Hydrolysis involves the consumption of water to break down macromolecules.

    <p>True (A)</p> Signup and view all the answers

    What type of bond is formed during the condensation of two monosaccharides?

    <p>Glycosidic bond</p> Signup and view all the answers

    Lipids are classified as __________, phospholipids, and related molecules.

    <p>simple lipids</p> Signup and view all the answers

    Match the type of lipid with its description:

    <p>Simple lipids = Fats, oils, and waxes Phospholipids = Major components of cell membranes Neutral fats = Stored energy Steroids = Hormonal functions</p> Signup and view all the answers

    Which of the following statements is true regarding lipids?

    <p>Lipids are hydrophobic and insoluble in water. (C)</p> Signup and view all the answers

    Glycerol and fatty acids are components of triglycerides.

    <p>True (A)</p> Signup and view all the answers

    The process that releases energy by splitting a disaccharide into two monosaccharides is known as __________.

    <p>hydrolysis</p> Signup and view all the answers

    What is the primary composition of starch?

    <p>25-30% amylose and 70-75% amylopectin (C)</p> Signup and view all the answers

    Glycogen is less extensively branched than amylopectin.

    <p>False (B)</p> Signup and view all the answers

    What type of bonds are primarily found in the structure of starch and glycogen?

    <p>glycosidic bonds</p> Signup and view all the answers

    Starch is an energy storage molecule in __________.

    <p>plants</p> Signup and view all the answers

    Match the following components with their descriptions:

    <p>Amylase = Enzyme that breaks down starch Glycogen = Energy storage in animals Amylose = Unbranched form of starch Amylopectin = Branched form of starch</p> Signup and view all the answers

    Which statement accurately describes glycogen?

    <p>It is predominantly found in liver and muscle cells. (B)</p> Signup and view all the answers

    Starch can be easily hydrolyzed to release glucose when needed.

    <p>True (A)</p> Signup and view all the answers

    The two types of glycosidic bonds in glycogen are 1-4 and 1-________ bonds.

    <p>6</p> Signup and view all the answers

    Study Notes

    Cell Structure and Function

    • Cells are the fundamental building blocks of all living organisms
    • Cells vary greatly in size and shape depending on their specific function
    • Cells are organized into tissues, organs, and organ systems
    • Different kinds of cells have specialized structures that enable them to perform specific functions
    • Cells are surrounded by a selectively permeable membrane
    • The membrane regulates the movement of substances into and out of the cell
    • Cells contain a variety of organelles within the cytoplasm, each with specific structures
    • Eukaryotic cells contain membrane-bound organelles such as the nucleus, Golgi apparatus, mitochondria, and chloroplasts
    • Prokaryotic cells do not contain membrane-bound organelles

    Organelle Structure and Function

    • Nucleus: Control center of the cell, contains DNA, responsible for the cell's activities
    • Mitochondria: Powerhouse of the cell, responsible for cellular respiration and energy production
    • Ribosomes: Synthesize proteins, found free in cytoplasm or attached to the endoplasmic reticulum
    • Endoplasmic Reticulum (ER): Network of membranes involved in protein and lipid synthesis; Rough ER has ribosomes, Smooth ER does not
    • Golgi apparatus: Modifies, sorts, and packages proteins and lipids for transport to other locations
    • Lysosomes: Contain enzymes for intracellular digestion and recycling of cellular components
    • Vacuoles: Fluid-filled sacs that store water, nutrients, and waste products, large in plant cells

    Cell Membranes

    • Structure: Phospholipid bilayer with embedded proteins, cholesterol, glycoproteins, etc.
    • Function: Selectively permeable membrane, regulates material transfer, cell-cell recognition
    • Fluid Mosaic Model: describes the fluidity of the membrane with molecules moving within the bilayer

    Cell Transport

    • Passive Transport mechanisms do not require energy
    • Active Transport mechanisms require energy
    • Diffusion (passive): movement of molecules from high concentration to low concentration, down a concentration gradient
    • Osmosis (passive): diffusion of water across a semipermeable membrane
    • Facilitated diffusion (passive): movement of molecules with the help of carrier proteins
    • Active transport: movement of molecules against a concentration gradient, using energy (e.g., sodium-potassium pumps)
    • Cytosis (active): large molecules are transported by vesicles into or out of the cell (e.g., endocytosis, exocytosis)

    Carbohydrates, Lipids, and Proteins

    • Carbohydrates: organic molecules containing carbon, hydrogen, and oxygen, typically in a 1:2:1 ratio. They serve as an energy source and structural components.
    • Lipids: organic molecules containing carbon, hydrogen, and oxygen. They are primarily hydrophobic (insoluble in water). They serve as a major energy source and as components of cell membranes. Types include fats, oils, phospholipids, and steroids.
    • Proteins: large, complex organic molecules made up of amino acids. Proteins have diverse roles in the body, including as enzymes, structural components, transporters, and more.

    Cell Size and Function

    • Cell size is limited by the diffusion rate of molecules in and out of the cell.
    • Specialized exchange surfaces increase surface area for organisms that are larger.

    Eukaryotic vs Prokaryotic Cells

    • Eukaryotic: membrane-bound organelles, multicellular, complex
    • Prokaryotic: no membrane-bound organelles, unicellular, simple

    Viruses

    • Non-cellular infectious agents, composed of genetic material (DNA or RNA) enclosed in a protein coat (capsid)
    • Viruses need a host cell to reproduce

    Protists

    • Eukaryotic organisms that are not plants, animals, or fungi. Includes single-celled organisms and some multicellular forms.
    • Exhibit diverse forms and functions, including photosynthesis, predation, and decomposition

    Important Biological Molecules

    • Organic compounds contain carbon: crucial role in the structure and function of many biological molecules.
    • Inorganic molecules do not contain carbon: still important in biological processes.
    • The four main macromolecules are: carbohydrates, lipids, proteins, and nucleic acids.

    Enzyme Function and Regulation

    • Enzymes are biological catalysts, speeding up chemical reactions without being consumed.
    • Enzyme activity is influenced by factors such as temperature, pH, and substrate concentration.
    • Enzyme regulation helps control metabolic pathways in cells.
    • Different types of inhibitors control enzyme activity in cells.
    • The active site of enzymes interacts with specific substrate molecules; an enzyme-substrate complex forms and enzymes catalyze reactions by lowering activation energy.
    • The structure of the enzyme determines its specificity and function.

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