Microscopy Principles and Applications

Passes visible light through the specimen, making denser areas appear darker (correct)

Emits specific wavelengths of light to excite fluorophores

Utilizes reflected light to create a bright image on a dark background

Enhances contrast by exploiting phase differences in light

Phase contrast microscope

Fluorescent microscope

Dark field microscope (correct)

Bright field microscope

To create a high magnification of electron-dense specimens

To enhance contrast without staining by exploiting phase differences in light (correct)

To illuminate specimens using fluorescent light

To measure the size of microorganisms

<p>Detecting target molecules and studying protein localization</p> Signup and view all the answers

<p>It uses electrons instead of light to form detailed images</p> Signup and view all the answers