Microscopy Principles and Applications

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Questions and Answers

What principle does a bright field microscope operate on?

Passes visible light through the specimen, making denser areas appear darker (correct)
Emits specific wavelengths of light to excite fluorophores
Utilizes reflected light to create a bright image on a dark background
Enhances contrast by exploiting phase differences in light

Which microscope is best suited for studying live, unstained microorganisms?

Phase contrast microscope
Fluorescent microscope
Dark field microscope (correct)
Bright field microscope

What is the primary function of a phase contrast microscope?

To create a high magnification of electron-dense specimens
To enhance contrast without staining by exploiting phase differences in light (correct)
To illuminate specimens using fluorescent light
To measure the size of microorganisms

What application is fluorescent microscopy commonly used for?

<p>Detecting target molecules and studying protein localization (C)</p> Signup and view all the answers

How does an electron microscope differ from a light microscope?

<p>It uses electrons instead of light to form detailed images (C)</p> Signup and view all the answers

Flashcards

Bright Field Microscope

A type of light microscope that uses visible light passing through a specimen, making denser areas appear darker.

Dark Field Microscope

This microscope illuminates the specimen from the sides, making it appear bright against a dark background, ideal for observing unstained living cells.

Phase Contrast Microscope

This microscope uses differences in the phase of light waves passing through the specimen to enhance contrast, allowing observation of unstained living cells with internal structures.

Fluorescent Microscope

A microscope that uses light and fluorescent dyes to illuminate specific molecules within a sample.

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Electron Microscope

A microscope that uses a beam of electrons to illuminate the specimen, providing extremely high magnification and resolution to reveal incredibly small details.

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