Microscopy Principles and Uses Quiz

Microscope Types and Techniques

• There are two main types of microscopes: Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM).
• SEM is analogous to a dissecting light microscope, while TEM is analogous to a compound light microscope.
• The total magnification in a microscope is the product of the ocular lens power and the objective lens power.
• Rules for microscope use include keeping both eyes open, using lens paper for cleaning, and not tilting the microscope when viewing.
• Focusing the microscope involves starting with the lowest power objective and using the coarse and fine adjustment knobs.
• Compound light microscopes are parfocal, meaning that once an object is in focus with the low power field, it should be almost in focus with the higher power.
• Inversion in microscopy refers to the fact that a microscopic image is upside down and reversed.
• The field of view is the area of the slide visible through the microscope's eyepiece and is shown as a circle.
• The field of view of the high power is 1/4 of the low power, and its diameter can be calculated using a ruler and converted to micrometers.
• Microscopic observations can be made using wet mount preparation to observe human epithelial cells and onion epidermal cells.
• Gram staining is used to distinguish and classify bacteria species into gram-positive and gram-negative bacteria.
• Other staining techniques include Giemsa staining, which is used for peripheral blood smear and bone marrow specimens, and Hematoxylin & Eosin staining, which is widely used in histology for medical diagnosis.

Microscope Types and Techniques

• There are two main types of microscopes: Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM).
• SEM is analogous to a dissecting light microscope, while TEM is analogous to a compound light microscope.
• The total magnification in a microscope is the product of the ocular lens power and the objective lens power.
• Rules for microscope use include keeping both eyes open, using lens paper for cleaning, and not tilting the microscope when viewing.
• Focusing the microscope involves starting with the lowest power objective and using the coarse and fine adjustment knobs.
• Compound light microscopes are parfocal, meaning that once an object is in focus with the low power field, it should be almost in focus with the higher power.
• Inversion in microscopy refers to the fact that a microscopic image is upside down and reversed.
• The field of view is the area of the slide visible through the microscope's eyepiece and is shown as a circle.
• The field of view of the high power is 1/4 of the low power, and its diameter can be calculated using a ruler and converted to micrometers.
• Microscopic observations can be made using wet mount preparation to observe human epithelial cells and onion epidermal cells.
• Gram staining is used to distinguish and classify bacteria species into gram-positive and gram-negative bacteria.
• Other staining techniques include Giemsa staining, which is used for peripheral blood smear and bone marrow specimens, and Hematoxylin & Eosin staining, which is widely used in histology for medical diagnosis.