Microscopy: Magnification vs. Resolution

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<p>False</p> Signup and view all the answers

<p>False</p> Signup and view all the answers

<p>True</p> Signup and view all the answers

<p>The fluorescent protein may not be expressed, or the correct excitation wavelength is not being used. To correct this, check the microscope settings, ensure the protein is expressed, and use the appropriate filters and light source.</p> Signup and view all the answers

<p>A fluorophore is a fluorescent chemical compound that can re-emit light upon light excitation. It is important in fluorescence microscopy because it allows for the visualization of specific components within a sample by emitting light when excited by a specific wavelength.</p> Signup and view all the answers

<p>Immunolabelling uses antibodies to target specific proteins, while GFP engineering involves using genetically encoded fluorescent proteins to visualize proteins in live cells. GFP allows you to view live cells. Immunolabelling can be faster, less difficult, but it can be used with both light and electron microscopy, unlike GFP.</p> Signup and view all the answers

<p>GFP cannot be used in electron microscopy because it requires light to fluoresce, and electron microscopy uses electron beams instead of light. GFP is ONLY used in fluorescence microscopy.</p> Signup and view all the answers

<p>Light field microscopy uses photons, while electron microscopy uses electrons.</p> Signup and view all the answers

<p>TEM</p> Signup and view all the answers

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<p>The sample appears dark in Transmission Electron Microscopy (TEM) because electrons are either absorbed or scattered by the denser regions of the sample, leading to fewer electrons reaching the detector.</p> Signup and view all the answers

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