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Questions and Answers
What is the key difference between an interference microscope and a phase-contrast microscope?
What is the key difference between an interference microscope and a phase-contrast microscope?
- Interference microscope utilizes prisms that split the light beam, while phase-contrast microscope does not
- Phase-contrast microscope is used for examining living specimens, while interference microscope is used for thin tissue slices
- Interference microscope has higher resolution than phase-contrast microscope
- Interference microscope uses two beams of light, while phase-contrast microscope uses one beam of light (correct)
How does a fluorescence microscope create images of microorganisms?
How does a fluorescence microscope create images of microorganisms?
- By using prisms to split the light beam
- By staining microorganisms with fluorochromes
- By using ultraviolet light to make microorganisms luminescent (correct)
- By utilizing interference of light waves
Which type of microscopy is best suited for examining living cells without staining?
Which type of microscopy is best suited for examining living cells without staining?
- Phase-contrast microscopy (correct)
- Fluorescence microscopy
- Bright-field microscopy
- Interference microscopy
What property of substances do fluorescence microscopes take advantage of?
What property of substances do fluorescence microscopes take advantage of?
In microscopy, what is the primary purpose of utilizing differences in refractive indices?
In microscopy, what is the primary purpose of utilizing differences in refractive indices?
What role do prisms play in an interference microscope's operation?
What role do prisms play in an interference microscope's operation?
Which type of microscopy is commonly used when standard staining procedures might affect biological processes in living specimens?
Which type of microscopy is commonly used when standard staining procedures might affect biological processes in living specimens?
How does an interference microscope differ from a bright-field microscope?
How does an interference microscope differ from a bright-field microscope?
What characteristic distinguishes fluorescence microscopy from phase-contrast microscopy?
What characteristic distinguishes fluorescence microscopy from phase-contrast microscopy?
Which type of microscopy is most suitable for visualizing subcellular particles like nuclei and organelles without staining?
Which type of microscopy is most suitable for visualizing subcellular particles like nuclei and organelles without staining?
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