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10 Questions

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Properties of Light Microscopy

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Questions and Answers

What is the key difference between an interference microscope and a phase-contrast microscope?

Interference microscope utilizes prisms that split the light beam, while phase-contrast microscope does not

Phase-contrast microscope is used for examining living specimens, while interference microscope is used for thin tissue slices

Interference microscope has higher resolution than phase-contrast microscope

Interference microscope uses two beams of light, while phase-contrast microscope uses one beam of light (correct)

How does a fluorescence microscope create images of microorganisms?

By using prisms to split the light beam

By staining microorganisms with fluorochromes

By using ultraviolet light to make microorganisms luminescent (correct)

By utilizing interference of light waves

Which type of microscopy is best suited for examining living cells without staining?

Phase-contrast microscopy (correct)

Fluorescence microscopy

Bright-field microscopy

Interference microscopy

What property of substances do fluorescence microscopes take advantage of?

Absorption of short wavelengths and emission of longer wavelengths Signup and view all the answers

In microscopy, what is the primary purpose of utilizing differences in refractive indices?

To create contrast in the image Signup and view all the answers

What role do prisms play in an interference microscope's operation?

Split the light beam to produce contrast colors Signup and view all the answers

Which type of microscopy is commonly used when standard staining procedures might affect biological processes in living specimens?

Fluorescence microscopy Signup and view all the answers

How does an interference microscope differ from a bright-field microscope?

Interference microscope utilizes prisms to split the light beam, while bright-field does not Signup and view all the answers

What characteristic distinguishes fluorescence microscopy from phase-contrast microscopy?

Utilization of ultraviolet light to make objects luminescent Signup and view all the answers

Which type of microscopy is most suitable for visualizing subcellular particles like nuclei and organelles without staining?

Phase-contrast microscopy Signup and view all the answers