Questions and Answers
What is the function of the eyepiece in a light microscope?
Magnifies the image by 10X, 12X, or 20X
What is the function of the objectives in a light microscope?
Magnify the material being examined
What is the function of the nose piece in a light microscope?
Rotates to allow for the changing of objectives to increase or decrease magnification
What is the function of the arm in a light microscope?
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What is the formula for total magnification in a light microscope?
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What is the alternative name for the eyepiece in a light microscope?
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What is the alternative name for the total magnification in a light microscope?
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What is the alternative name for the nose piece in a light microscope?
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What is the function of the pointer in the eyepiece of a light microscope?
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What is the function of the adjustment for inter-pupillary distance in the eyepiece of a light microscope?
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What is the function of the oil immersion objective in a light microscope?
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What is the function of the stage control knobs in a light microscope?
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What is the function of the substage condenser in a light microscope?
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What is the purpose of the iris diaphragm in a light microscope?
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What is the function of the coarse focusing knob in a light microscope?
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What is the role of the fine focusing knob in a light microscope?
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What is the purpose of the condenser control knob in a light microscope?
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What is the function of the illuminator in a light microscope?
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What is the role of the base in a light microscope?
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What is resolution (resolving power) in a light microscope?
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What is the maximum magnification of a light microscope?
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How is the total magnification calculated in a light microscope?
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What is the purpose of focusing the microscope?
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What is the function of the substage condenser in a light microscope?
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What is the purpose of the iris diaphragm in a light microscope?
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What is the function of the coarse focusing knob in a light microscope?
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What is the function of the fine focusing knob in a light microscope?
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What is resolution (resolving power) in a light microscope?
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What is the function of the illuminator in a light microscope?
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What is the role of the base in a light microscope?
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What is the function of the stage control knobs in a light microscope?
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What is the function of the condenser control knob in a light microscope?
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What is the alternative name for the total magnification in a light microscope?
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What is the maximum magnification of a light microscope?
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What is the function of the oil immersion objective in a light microscope?
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Study Notes
Components of a Light Microscope
- The eyepiece is responsible for magnifying the image formed by the objectives.
- The alternative name for the eyepiece is ocular.
- The eyepiece has a pointer that helps to locate the object being viewed.
- The adjustment for inter-pupillary distance in the eyepiece allows the user to adjust the distance between the lenses to fit their eyes.
Objectives
- The objectives are responsible for collecting and focusing light from the specimen.
- The oil immersion objective is used to increase the resolution of the microscope by using oil to fill the space between the objective and the specimen.
Nose Piece
- The nose piece is a rotating turret that holds the objectives in place.
- The alternative name for the nose piece is objective revolver.
Arm
- The arm is the supporting pillar that connects the microscope to the base.
Magnification
- The formula for total magnification is the product of the magnification power of the eyepiece and the magnification power of the objective.
- The alternative name for total magnification is overall magnification.
- The maximum magnification of a light microscope is typically around 1000-1500x.
Focusing
- The coarse focusing knob is used to bring the specimen into rough focus.
- The fine focusing knob is used to make fine adjustments to the focus.
- The purpose of focusing the microscope is to produce a clear and sharp image of the specimen.
Stage and Condenser
- The stage control knobs are used to move the stage and position the specimen.
- The substage condenser is used to focus the light onto the specimen.
- The condenser control knob is used to adjust the height of the substage condenser.
- The iris diaphragm is used to control the amount of light that enters the microscope.
Base and Illuminator
- The base provides stability and support for the microscope.
- The illuminator is the light source that provides light to the microscope.
Resolution
- Resolution (resolving power) is the ability of the microscope to distinguish between two closely spaced objects.
- The resolution of a light microscope is limited by the wavelength of light used.
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