Microscopy and Spherical Aberration Quiz
14 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What does the abbreviation 'Fluar' indicate in microscopy?

  • Water immersion
  • Fluorite aberration correction (correct)
  • Fluorescence imaging capability
  • Long working distance
  • What feature does the abbreviation 'UPLAN' represent in microscopy objectives?

  • Universal Plan for different lighting techniques (correct)
  • Adjustable numerical aperture
  • Super-Long Working Distance
  • Flat Field optical correction
  • What function is associated with the abbreviation 'W/Iris' on a microscope objective?

  • Water Immersion capability
  • Differential Interference Contrast capability
  • Adjustable numerical aperture with iris diaphragm (correct)
  • Phase Contrast imaging
  • Which abbreviation indicates a correction collar for optical aberrations in microscopy objectives?

    <p>Corr</p> Signup and view all the answers

    What is the main consequence of spherical aberration in confocal microscopy?

    <p>Apparent loss of fluorescence intensity</p> Signup and view all the answers

    How does the use of water as an objective immersion medium help address spherical aberration?

    <p>It increases the depth of imaging into a sample</p> Signup and view all the answers

    What effect does spherical aberration have on the quality of the images obtained in microscopy?

    <p>Compromises the fluorescent signal</p> Signup and view all the answers

    How do objectives that use water as an immersion medium differ from oil immersion objectives in addressing spherical aberration?

    <p>Oil objectives allow imaging deeper into samples</p> Signup and view all the answers

    What does reflection of waves involve?

    <p>A change in direction of waves when they bounce off a barrier</p> Signup and view all the answers

    Which phenomenon involves a change in the direction of waves as they pass through an opening or around an obstacle?

    <p>Diffraction</p> Signup and view all the answers

    What determines the size of an Airy disk after light diffracts through a small circular aperture?

    <p>The wavelength of the light and the size of the aperture</p> Signup and view all the answers

    What condition is reached at the limit of resolution according to the Rayleigh criterion?

    <p>The principal maximum of object 1 coincides with first minimum of object 2</p> Signup and view all the answers

    What does the refractive index or index of refraction measure in optics?

    <p>The speed of light in that medium</p> Signup and view all the answers

    What leads to interference patterns such as Airy disks?

    <p>Diffraction</p> Signup and view all the answers

    More Like This

    Microscopy and Histology Quiz
    10 questions
    Microscope Aberrations and Focusing
    63 questions
    Microscopy Flashcards
    17 questions

    Microscopy Flashcards

    ExceedingSodalite avatar
    ExceedingSodalite
    Microscopy Basics Lesson 1-10
    17 questions
    Use Quizgecko on...
    Browser
    Browser