Microscopy and Spherical Aberration Quiz

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Questions and Answers

What does the abbreviation 'Fluar' indicate in microscopy?

  • Water immersion
  • Fluorite aberration correction (correct)
  • Fluorescence imaging capability
  • Long working distance

What feature does the abbreviation 'UPLAN' represent in microscopy objectives?

  • Universal Plan for different lighting techniques (correct)
  • Adjustable numerical aperture
  • Super-Long Working Distance
  • Flat Field optical correction

What function is associated with the abbreviation 'W/Iris' on a microscope objective?

  • Water Immersion capability
  • Differential Interference Contrast capability
  • Adjustable numerical aperture with iris diaphragm (correct)
  • Phase Contrast imaging

Which abbreviation indicates a correction collar for optical aberrations in microscopy objectives?

<p>Corr (A)</p> Signup and view all the answers

What is the main consequence of spherical aberration in confocal microscopy?

<p>Apparent loss of fluorescence intensity (A)</p> Signup and view all the answers

How does the use of water as an objective immersion medium help address spherical aberration?

<p>It increases the depth of imaging into a sample (B)</p> Signup and view all the answers

What effect does spherical aberration have on the quality of the images obtained in microscopy?

<p>Compromises the fluorescent signal (A)</p> Signup and view all the answers

How do objectives that use water as an immersion medium differ from oil immersion objectives in addressing spherical aberration?

<p>Oil objectives allow imaging deeper into samples (B)</p> Signup and view all the answers

What does reflection of waves involve?

<p>A change in direction of waves when they bounce off a barrier (B)</p> Signup and view all the answers

Which phenomenon involves a change in the direction of waves as they pass through an opening or around an obstacle?

<p>Diffraction (D)</p> Signup and view all the answers

What determines the size of an Airy disk after light diffracts through a small circular aperture?

<p>The wavelength of the light and the size of the aperture (C)</p> Signup and view all the answers

What condition is reached at the limit of resolution according to the Rayleigh criterion?

<p>The principal maximum of object 1 coincides with first minimum of object 2 (A)</p> Signup and view all the answers

What does the refractive index or index of refraction measure in optics?

<p>The speed of light in that medium (B)</p> Signup and view all the answers

What leads to interference patterns such as Airy disks?

<p>Diffraction (A)</p> Signup and view all the answers

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