Microscopy and Histology Quiz
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Questions and Answers

Which microscope would be particularly useful for looking at living cells?

  • Compound microscope
  • Transmission electron microscope
  • Simple microscope
  • Phase contrast microscope (correct)
  • Which of the following stain purple blue with H&E stain?

  • Elastic fibers
  • Nucleus (correct)
  • Collagen fibers
  • Cytoplasm
  • What are Sudan stains used primarily for?

  • Fat (correct)
  • Elastic fibers
  • Blood
  • Nervous tissue
  • During the preparation of a routine H&E slide, what step occurs after the tissue is preserved (fixation)?

    <p>Embedding in paraffin</p> Signup and view all the answers

    During the preparation of a routine H&E slide, what step occurs after the tissue is sectioned?

    <p>Staining</p> Signup and view all the answers

    What is the primary function of Sudan stains?

    <p>Fat</p> Signup and view all the answers

    What step occurs after the tissue is preserved (fixation) during the preparation of a routine H&E slide?

    <p>Embedding in paraffin</p> Signup and view all the answers

    Which of the following is an acid dye?

    <p>Eosin</p> Signup and view all the answers

    Which stain is used for routine histological examination?

    <p>Hematoxylin and eosin stain</p> Signup and view all the answers

    What is the primary component of the cell coat?

    <p>Lipids and proteins</p> Signup and view all the answers

    Study Notes

    Microscopy and Staining

    • Phase contrast microscopy is particularly useful for looking at living cells.
    • Basophilic structures (e.g., nuclei and RNA-rich regions) stain purple-blue with H&E stain.
    • Sudan stains are primarily used for staining lipids (fats).

    H&E Slide Preparation

    • After preservation (fixation), the tissue is dehydrated during the preparation of a routine H&E slide.
    • After the tissue is sectioned, it is mounted on a slide during the preparation of a routine H&E slide.

    Stains and Dyes

    • Sudan stains' primary function is to stain lipids (fats).
    • Hematoxylin is a basic dye.
    • Eosin is an acid dye.
    • H&E stain is used for routine histological examination.

    Cell Structure

    • The primary component of the cell coat (glycocalyx) is carbohydrates.

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    Description

    Test your knowledge on microscopy and histology with this quiz. Questions cover topics such as types of microscopes, staining techniques, and tissue preparation processes.

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