10 Questions
Which microscope would be particularly useful for looking at living cells?
Phase contrast microscope
Which of the following stain purple blue with H&E stain?
Nucleus
What are Sudan stains used primarily for?
Fat
During the preparation of a routine H&E slide, what step occurs after the tissue is preserved (fixation)?
Embedding in paraffin
During the preparation of a routine H&E slide, what step occurs after the tissue is sectioned?
Staining
What is the primary function of Sudan stains?
Fat
What step occurs after the tissue is preserved (fixation) during the preparation of a routine H&E slide?
Embedding in paraffin
Which of the following is an acid dye?
Eosin
Which stain is used for routine histological examination?
Hematoxylin and eosin stain
What is the primary component of the cell coat?
Lipids and proteins
Study Notes
Microscopy and Staining
- Phase contrast microscopy is particularly useful for looking at living cells.
- Basophilic structures (e.g., nuclei and RNA-rich regions) stain purple-blue with H&E stain.
- Sudan stains are primarily used for staining lipids (fats).
H&E Slide Preparation
- After preservation (fixation), the tissue is dehydrated during the preparation of a routine H&E slide.
- After the tissue is sectioned, it is mounted on a slide during the preparation of a routine H&E slide.
Stains and Dyes
- Sudan stains' primary function is to stain lipids (fats).
- Hematoxylin is a basic dye.
- Eosin is an acid dye.
- H&E stain is used for routine histological examination.
Cell Structure
- The primary component of the cell coat (glycocalyx) is carbohydrates.
Test your knowledge on microscopy and histology with this quiz. Questions cover topics such as types of microscopes, staining techniques, and tissue preparation processes.
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