Podcast
Questions and Answers
Which microscope would be particularly useful for looking at living cells?
Which microscope would be particularly useful for looking at living cells?
- Compound microscope
- Transmission electron microscope
- Simple microscope
- Phase contrast microscope (correct)
Which of the following stain purple blue with H&E stain?
Which of the following stain purple blue with H&E stain?
- Elastic fibers
- Nucleus (correct)
- Collagen fibers
- Cytoplasm
What are Sudan stains used primarily for?
What are Sudan stains used primarily for?
- Fat (correct)
- Elastic fibers
- Blood
- Nervous tissue
During the preparation of a routine H&E slide, what step occurs after the tissue is preserved (fixation)?
During the preparation of a routine H&E slide, what step occurs after the tissue is preserved (fixation)?
During the preparation of a routine H&E slide, what step occurs after the tissue is sectioned?
During the preparation of a routine H&E slide, what step occurs after the tissue is sectioned?
What is the primary function of Sudan stains?
What is the primary function of Sudan stains?
What step occurs after the tissue is preserved (fixation) during the preparation of a routine H&E slide?
What step occurs after the tissue is preserved (fixation) during the preparation of a routine H&E slide?
Which of the following is an acid dye?
Which of the following is an acid dye?
Which stain is used for routine histological examination?
Which stain is used for routine histological examination?
What is the primary component of the cell coat?
What is the primary component of the cell coat?
Flashcards
Living cells microscope
Living cells microscope
A phase contrast microscope is best for observing living cells, as it highlights differences in refractive index without staining.
H&E stain nucleus color
H&E stain nucleus color
The nucleus stains purple-blue with Hematoxylin and Eosin (H&E) stain.
Sudan stain use
Sudan stain use
Sudan stains are primarily used to visualize fat.
H&E paraffin embedding
H&E paraffin embedding
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H&E staining step
H&E staining step
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Sudan stain function
Sudan stain function
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Paraffin embedding after
Paraffin embedding after
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Acid dye example
Acid dye example
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Routine histological stain
Routine histological stain
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Cell coat components
Cell coat components
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Study Notes
Microscopy and Staining
- Phase contrast microscopy is particularly useful for looking at living cells.
- Basophilic structures (e.g., nuclei and RNA-rich regions) stain purple-blue with H&E stain.
- Sudan stains are primarily used for staining lipids (fats).
H&E Slide Preparation
- After preservation (fixation), the tissue is dehydrated during the preparation of a routine H&E slide.
- After the tissue is sectioned, it is mounted on a slide during the preparation of a routine H&E slide.
Stains and Dyes
- Sudan stains' primary function is to stain lipids (fats).
- Hematoxylin is a basic dye.
- Eosin is an acid dye.
- H&E stain is used for routine histological examination.
Cell Structure
- The primary component of the cell coat (glycocalyx) is carbohydrates.
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Description
Test your knowledge on microscopy and histology with this quiz. Questions cover topics such as types of microscopes, staining techniques, and tissue preparation processes.
