Microscopes: Simple vs. Compound
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Questions and Answers

What is the total magnification when using a X10 ocular lens and a X40 objective lens on a compound light microscope?

  • X200
  • X1600
  • X1000
  • X400 (correct)
  • How would replacing the condenser with a darkfield condenser impact the view in a compound light microscope?

  • Objects will be observed against a dark background. (correct)
  • Objects will appear smaller.
  • Objects will be observed against a bright background.
  • Objects will become more focused.
  • What are photomicrographs?

  • Photographs taken using digital cameras.
  • Photographs taken under direct sunlight.
  • Photographs taken through a telescope lens.
  • Photographs taken through the lens system of a compound light microscope. (correct)
  • What term is used to refer to the microscope when objects are observed against a bright background?

    <p>Brightfield microscope</p> Signup and view all the answers

    Which component in a compound light microscope impacts whether objects are seen against a dark or bright background?

    <p>Condenser</p> Signup and view all the answers

    If an X10 ocular lens is used with an X100 objective lens, what is the total magnification achieved?

    <p>X1000</p> Signup and view all the answers

    What is the role of the eyepiece or ocular lens in a compound light microscope?

    <p>To magnify the image further for observation.</p> Signup and view all the answers

    What is the resolving power of a compound light microscope?

    <p>The ability to distinguish two separate points as distinct entities</p> Signup and view all the answers

    What is one of the limitations of visible light in microscopy?

    <p>Its inability to penetrate thick specimens effectively</p> Signup and view all the answers

    What happens when an object is observed under dark field illumination in a microscope?

    <p>The object appears brighter against a dark background.</p> Signup and view all the answers

    Study Notes

    Simple Microscopes

    • Contain only one magnifying lens
    • A magnifying glass is an example of a simple microscope
    • Images appear 3-20 times larger than the object's actual size
    • Leeuwenhoek's simple microscopes had a maximum magnifying power of about X300 (300 times)

    Compound Microscopes

    • Contain more than one magnifying lens
    • Also referred to as compound light microscopes due to the use of visible light as the source of illumination
    • Usually magnify objects about 1000 times
    • Resolving power is approximately 0.2 µm (about 1,000 times better than the unaided human eye)
    • Limited by the wavelength of visible light (~0.45 µm), which determines the minimum size of objects that can be seen

    Improving Contrast in Light Microscopy

    • Contrast improvement is essential for a clear final image
    • Staining is a common method to improve contrast in bright field microscopy
    • Dyes used for staining are organic compounds that bind to specific cellular materials
    • Basic dyes are positively charged and bind to negatively charged components like nucleic acids and acidic polysaccharides
    • Staining can, however, kill cells and distort their features

    Types of Staining Procedures

    • Simple stain
    • Differential stain
    • Structural stains

    Electron Microscopes

    • Enable the observation of extremely small microbes like rabies and smallpox viruses
    • Cannot be used to observe living organisms, as the processing procedures are lethal to cells
    • Use an electron beam as the source of illumination and magnets to focus the beam
    • Have a much higher resolving power than compound light microscopes
    • Come in two types: transmission and scanning electron microscopes

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    Description

    Learn about the differences between simple microscopes, which contain only one magnifying lens, and compound microscopes, which contain multiple lenses for higher magnification.

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