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Questions and Answers
What is the main characteristic of a simple microscope?
What is the main characteristic of a simple microscope?
Which type of microscope is also known as a compound light microscope?
Which type of microscope is also known as a compound light microscope?
What is the resolving power of a compound light microscope?
What is the resolving power of a compound light microscope?
What is the main advantage of electron microscopes over compound light microscopes?
What is the main advantage of electron microscopes over compound light microscopes?
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What limits the size of objects that can be seen through visible light in microscopes?
What limits the size of objects that can be seen through visible light in microscopes?
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Which type of microscope uses an electron beam as the source of illumination?
Which type of microscope uses an electron beam as the source of illumination?
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What is the total magnification usually achieved by compound light microscopes?
What is the total magnification usually achieved by compound light microscopes?
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What distinguishes a simple microscope from a compound microscope in terms of the number of magnifying lenses?
What distinguishes a simple microscope from a compound microscope in terms of the number of magnifying lenses?
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What is the main limitation of using staining procedures in microscopy?
What is the main limitation of using staining procedures in microscopy?
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What type of dyes are commonly used to stain cells and increase contrast in a bright field microscope?
What type of dyes are commonly used to stain cells and increase contrast in a bright field microscope?
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Which component of cells do basic dyes bind strongly to?
Which component of cells do basic dyes bind strongly to?
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What aspect of an object is observed using a brightfield microscope?
What aspect of an object is observed using a brightfield microscope?
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What is the total magnification when using a X10 ocular lens with a X40 objective lens in a compound microscope?
What is the total magnification when using a X10 ocular lens with a X40 objective lens in a compound microscope?
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Which component of a compound microscope is responsible for changing the background from bright to dark in darkfield microscopy?
Which component of a compound microscope is responsible for changing the background from bright to dark in darkfield microscopy?
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Why can't living organisms be observed using an electron microscope?
Why can't living organisms be observed using an electron microscope?
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What term is used to describe photographs taken through the lens system of a compound light microscope?
What term is used to describe photographs taken through the lens system of a compound light microscope?
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If the objective lens used in a compound microscope has a magnification of X100, what is the total magnification when combined with a X10 ocular lens?
If the objective lens used in a compound microscope has a magnification of X100, what is the total magnification when combined with a X10 ocular lens?
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When using a darkfield condenser in a compound microscope, how are illuminated objects seen?
When using a darkfield condenser in a compound microscope, how are illuminated objects seen?
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Study Notes
Types of Microscopes
- A simple microscope contains a single magnifying lens.
- Compound light microscope is also known for its use of multiple lenses to magnify objects.
- Electron microscopes utilize an electron beam for illumination, allowing for much higher magnification.
Magnification and Resolution
- The resolving power of a compound light microscope is generally around 200 nanometers.
- Total magnification with a compound microscope can reach up to 1000x, typically using an ocular lens of 10x with various objective lenses.
- When using a X10 ocular lens and a X40 objective lens, total magnification is 400x.
- For a X10 ocular lens with a X100 objective lens, total magnification is 1000x.
Limitations and Advantages
- The main advantage of electron microscopes is their ability to resolve much finer details due to shorter wavelength of electrons compared to visible light.
- The size of objects visible through light microscopes is limited by the wavelength of visible light, which restricts the ability to see structures smaller than approximately 200 nanometers.
- Staining procedures can introduce artifacts and alter cellular structures, which limits accurate observation.
Staining Techniques
- Common dyes used in bright field microscopy include basic dyes, which help to enhance contrast.
- Basic dyes bind strongly to nucleic acids and other cellular components, which are negatively charged.
- A brightfield microscope observes the overall shape and structure of cells.
Special Techniques and Observations
- Darkfield microscopy utilizes a special condenser to change the background from bright to dark, enhancing visibility of illuminated objects against a dark background.
- Objects appear brighter and more distinct against a dark field in darkfield microscopy.
- Living organisms cannot be observed under electron microscopes because the sample must be vacuum-dried and cannot retain life.
Imaging
- Photographs taken through the lens system of a compound light microscope are known as photomicrographs.
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Description
Learn about the differences between simple and compound microscopes, including their magnifying powers and components. Understand how Leeuwenhoek's simple microscopes and modern compound microscopes work.
