18 Questions
What is the main characteristic of a simple microscope?
Has a maximum magnifying power of about X300
Which type of microscope is also known as a compound light microscope?
Compound microscope
What is the resolving power of a compound light microscope?
Approximate 0.2 µm
What is the main advantage of electron microscopes over compound light microscopes?
Higher resolving power
What limits the size of objects that can be seen through visible light in microscopes?
~0.45 µm wavelength of visible light
Which type of microscope uses an electron beam as the source of illumination?
Electron microscope
What is the total magnification usually achieved by compound light microscopes?
~1000 times
What distinguishes a simple microscope from a compound microscope in terms of the number of magnifying lenses?
Simple microscope has more magnifying lenses
What is the main limitation of using staining procedures in microscopy?
Killing cells and distorting their features
What type of dyes are commonly used to stain cells and increase contrast in a bright field microscope?
Basic dyes
Which component of cells do basic dyes bind strongly to?
Nucleic acids and acidic polysaccharides
What aspect of an object is observed using a brightfield microscope?
Objects against a bright background
What is the total magnification when using a X10 ocular lens with a X40 objective lens in a compound microscope?
X400
Which component of a compound microscope is responsible for changing the background from bright to dark in darkfield microscopy?
Condenser
Why can't living organisms be observed using an electron microscope?
The processing procedures kill the organisms
What term is used to describe photographs taken through the lens system of a compound light microscope?
Photomicrographs
If the objective lens used in a compound microscope has a magnification of X100, what is the total magnification when combined with a X10 ocular lens?
X1000
When using a darkfield condenser in a compound microscope, how are illuminated objects seen?
Against a dark background
Learn about the differences between simple and compound microscopes, including their magnifying powers and components. Understand how Leeuwenhoek's simple microscopes and modern compound microscopes work.
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