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Questions and Answers
What is the primary function of the eyepiece in a microscope?
What is the primary function of the eyepiece in a microscope?
- To view the specimen at varying magnifications (correct)
- To adjust the light intensity
- To collect light for imaging
- To support the microscope's structure
Which part of the microscope allows for the rotation of multiple objective lenses?
Which part of the microscope allows for the rotation of multiple objective lenses?
- Stage clip
- Eyepiece tube
- Body tube
- Nose piece (correct)
What distinguishes fine adjustment knobs from coarse adjustment knobs?
What distinguishes fine adjustment knobs from coarse adjustment knobs?
- Fine adjustment requires more force than coarse adjustment.
- Fine adjustment moves the stage minimally, whereas coarse adjustment brings the specimen into general focus. (correct)
- Fine adjustment is not adjustable and remains fixed.
- Coarse adjustment is used for high magnifications only.
What role do condensers play in a microscope?
What role do condensers play in a microscope?
What is the standard magnification power of a typical eyepiece?
What is the standard magnification power of a typical eyepiece?
Which part of the microscope ensures that the specimen does not fall off the stage?
Which part of the microscope ensures that the specimen does not fall off the stage?
Identify the component responsible for generating light in a microscope.
Identify the component responsible for generating light in a microscope.
The body tube of a microscope connects which two components?
The body tube of a microscope connects which two components?
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Study Notes
Eyepiece (Ocular)
- Located at the top of the microscope; used for viewing.
- Standard magnification: 10x; optional magnifications range from 5x to 30x.
Eyepiece Tube
- Holds the eyepiece above the objective lenses.
- In binocular microscopes, it's flexible and rotatable for optimal viewing.
Objective Lenses
- Magnify the specimen at different intensities.
- Magnification power typically ranges from 4x to 100x.
Arm
- Connects all microscope components; provides support for proper use.
Body Tube
- Connects the eyepiece to the objective lenses.
Nosepiece
- Rotating mount holding multiple objective lenses; allows for magnification changes.
Adjustment Knobs
- Fine adjustment: Regulates the object-objective distance for sharpness; minimally moves the stage.
- Coarse adjustment: Brings the specimen into general focus quickly; roughly adjusts the object-objective distance.
Stage
- Platform where the specimen is placed for viewing.
Stage Clip
- Holds the specimen slide in place.
Aperture
- Hole in the microscope stage allowing transmitted light to reach the specimen.
Microscopic Illuminator
- Light source at the base, replacing a mirror.
- Uses a low-voltage (approx. 100V) external light source.
Condensers
- Lenses under the stage, focusing light from the illuminator onto the specimen.
- Crucial for sharp images at high magnifications (400x and above).
Diaphragm (Iris)
- Located under the stage; controls the amount of light reaching the specimen.
- Adjustable to control light intensity and beam size.
Base
- The bottom part of the microscope; supports all other components.
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