Microplate Reader Calibration and Maintenance Quiz

<p>The illumination lamp is malfunctioning. (A)</p> Signup and view all the answers

<p>Calibration of each one of the channels. (D)</p> Signup and view all the answers

<p>Check if TMB is colourless and preparation is adequate. (D)</p> Signup and view all the answers

<p>Clean the optical sensors. (D)</p> Signup and view all the answers

<p>To measure the success of the test (C)</p> Signup and view all the answers

<p>The calibration status column by column (A)</p> Signup and view all the answers

<p>Confirm the light emission and detection systems are properly aligned. (B)</p> Signup and view all the answers

<p>There is no difference between the average readings of columns (A)</p> Signup and view all the answers

<p>A nearby source of vibration (B)</p> Signup and view all the answers

<p>Perform a reading with an empty plate (A)</p> Signup and view all the answers

<p>There are many test-specific variants of ELISA (D)</p> Signup and view all the answers

<p>Supplying washing solution and extracting fluids (B)</p> Signup and view all the answers

<p>Phosphate buffer solution (PBS) (A)</p> Signup and view all the answers

<p>The test results could be inaccurate (D)</p> Signup and view all the answers

<p>1 to 3 litres (C)</p> Signup and view all the answers

<p>Centrifuge (A)</p> Signup and view all the answers

<p>0.3 to 0.5 mm (B)</p> Signup and view all the answers

<p>Better throughput and minimized handling of contamination (D)</p> Signup and view all the answers

<p>Extraction needle (C)</p> Signup and view all the answers

<p>2 months (A)</p> Signup and view all the answers

<p>Washing can be done with one to three needles depending on the washer model. (B)</p> Signup and view all the answers

<p>To convert the voltage from 110 V to 12 V (A)</p> Signup and view all the answers

<p>To control the wave type and ensure it is positive (C)</p> Signup and view all the answers

<p>Tri terminal regulators (D)</p> Signup and view all the answers

<p>To stabilize at high frequency (C)</p> Signup and view all the answers

<p>Using the equation Gain = 1 + (R3 + PxR4)/R5 + (1 – P) x R4 (C)</p> Signup and view all the answers

<p>The washer was not purged sufficiently at the beginning. (A)</p> Signup and view all the answers

<p>There is a damp or blocked line filter. (A)</p> Signup and view all the answers

<p>Replace the volume missing. (C)</p> Signup and view all the answers

<p>Residual solution remaining in the wells. (A)</p> Signup and view all the answers

<p>The diameter of the vacuum channels. (B)</p> Signup and view all the answers

<p>Check if the points reach the bottom of the wells. (C)</p> Signup and view all the answers

<p>Check the integrity of the suction line filter. (D)</p> Signup and view all the answers

<p>Exhausted washing solution reserve. (D)</p> Signup and view all the answers

<p>Reference Electrode (B)</p> Signup and view all the answers

<p>Transformative Capacitor (A)</p> Signup and view all the answers

<p>Mercury Chloride (B)</p> Signup and view all the answers

<p>7812 (D)</p> Signup and view all the answers

<p>To allow selective ion permeability (B)</p> Signup and view all the answers

<p>Electrode potential (C)</p> Signup and view all the answers

<p>TL081 (D)</p> Signup and view all the answers

<p>To allow ionic exchange (B)</p> Signup and view all the answers

<p>120V to 12V (D)</p> Signup and view all the answers

<p>Silver (C)</p> Signup and view all the answers

A process used to detect and quantify substances like proteins, antibodies, or hormones in a sample.

A small plastic plate with multiple wells, each capable of holding a sample for testing.

A machine that accurately reads the color change in the wells of an ELISA plate, providing quantitative results.

A device used to wash the wells of the ELISA plate after each step, removing excess reagents.

A controlled standard sample with a known concentration used to calibrate and evaluate the accuracy of the ELISA test.

A device used to dispense precise volumes of liquid into the wells of an ELISA plate.

A device used for controlled incubation of the ELISA plate, ensuring optimal reaction conditions.

A stable, clean workspace free from vibrations, dust, and any extraneous equipment.

A system that removes excess liquids and waste from microplate wells using suction.

A mechanism that moves needles vertically to dispense or extract solutions from wells.

A solution used to wash microplates, removing excess reagents and preventing reactions from continuing.

The process of removing excess reagents from microplate wells using suction and automated systems.

Small plastic plates with multiple wells, each designed to hold a sample for testing.

Techniques that use a single needle to dispense and extract solutions from microplates.

Techniques that use two needles for washing and extraction, one for supplying solution and one for removing excess.

A common issue in microplate readings, often caused by a dirty reading plate. This indicates a discrepancy between readings from different wells on the same plate.

A corrective measure to address reading variance on the same plate, suggesting the plate itself may be the culprit. This involves flipping the plate 180 degrees and re-reading to see if variations persist.

A sign of proper calibration, it demonstrates the reader's consistency and reliability. This is achieved when readings remain identical at two wavelengths, indicating the instrument's ability to provide accurate readings.

A routine process to ensure optimal instrument performance. This involves verifying the readings against a known standard and adjusting the settings accordingly.

A method to validate the instrument's calibration without calibration plates, involving a colored solution. Readings before and after inverting the plate should reveal identical average values in each row, indicating the instrument's reliability.

A common problem leading to unreliable readings, it can occur due to dust, contamination, or other factors. Cleaning these components ensures accurate readings.

A crucial component of instrument maintenance, ensuring optimal readings. Cleaning these components helps to maintain accurate light transmission and detection.

The main reason for high absorbance values, indicating possible reagent degradation or improper preparation. These problems affect the overall efficacy of the assay.

Residual solution remains in the wells after washing due to a problem with the washer's extraction system. This could be caused by a malfunctioning vacuum system, incorrectly sized vacuum lines, obstructions in the suction line, a full waste container, a damp or blocked line filter, incorrectly positioned needles, use of an inappropriate microplate, insufficient purging, incorrect operation, misalignment of the microplate, inadequate washing cycle, exhausted washing solution reserve, or insufficient initial purging.

Ensures that the vacuum system is functioning properly, with the correct pressure and diameter of channels, as well as clean vacuum lines and a properly sized waste container. It also involves checking and, if necessary, cleaning the filter, ensuring correct needle placement, using the appropriate microplate, and adequately purging and aligning the plate. Additionally, it requires verifying the washing solution level and adequately purging the system at the start of the work cycle to eliminate humidity and air bubbles.

A device that converts the voltage from 110 V AC to 12 V AC.

A semiconductor device that allows current to flow in one direction only. In this circuit, it ensures the output current is positive.

These capacitors store the DC voltage from the diodes, smoothing out the flow of current and providing a stable voltage.

A device that regulates the output voltage to a precise 12V DC. This ensures a stable and consistent voltage for the circuit.

These small capacitors help maintain stability at high frequencies in the circuit, preventing oscillations and ensuring smooth operation.

A type of electrode used in pH measurements. Its unique design allows it to measure pH directly, eliminating the need for a separate reference electrode.

A type of electrode that provides a stable reference potential against which the potential difference of the sensing electrode is compared. It typically consists of a mercury electrode in contact with a saturated solution of mercury chloride.

A type of electrode used in various electrochemical measurements, often employed as a reference electrode in certain pH meters. It usually consists of a platinum wire or a platinum black electrode.

A type of electrode used in reference electrodes for pH measurements. This electrode is known for its consistency and reliability in providing a stable counter-electrode for voltage measurements.

The solution used in reference electrodes to create a stable and consistent reference potential. This solution is crucial for accurate pH measurements.

A component of a combined electrode, this mesh is designed to allow ions to pass through while preventing the mixing of the internal electrode solution with the sample being measured.

The part of a combined electrode that connects the internal electrode solution to the sample being measured. It allows the pH to be measured accurately while preventing contamination of the internal solution.

A type of semiconductor device used in electronic circuits to regulate voltage and current. It acts as a voltage regulator and a current limiter within the pH meter, ensuring stable and reliable operation.

A type of transformer designed to convert alternating current (AC) to direct current (DC) for use in the pH meter, providing the necessary power for its operation.

A type of integrated circuit used in the pH meter to amplify the signal received from the pH electrode, enhancing the signal for accurate readings.