Microbiology_2
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Questions and Answers

What is the main criterion for classifying microorganisms into ACDP Categories?

  • The potential for genetic modification
  • Size of the microorganism
  • Growth rate of the microorganism
  • Severity of disease and availability of treatment (correct)
  • ACDP Category 2 organisms are characterized by which of the following?

  • There is no treatment available for infections caused by them.
  • They can cause severe human disease but cannot spread in the local population.
  • They can cause human disease and may present a hazard to the environment. (correct)
  • They are generally non-pathogenic and pose minimal hazards.
  • Which facility classification corresponds to ACDP Category 1 organisms?

  • BSL-0 facilities requiring no special precautions
  • BSL-1 facilities with general protective equipment (correct)
  • BSL-3 facilities requiring high-level containment
  • BSL-2 facilities with specific training requirements
  • Which of the following is NOT a characteristic of BSL-2 facilities?

    <p>Unrestricted access to laboratory areas (A)</p> Signup and view all the answers

    Under what condition might the general rule of thumb regarding BSL and ACDP level not apply?

    <p>If effective vaccination is available (D)</p> Signup and view all the answers

    Which of the following statements accurately describes a BSL-4 facility?

    <p>Organisms must be handled in a class 3 cabinet. (A)</p> Signup and view all the answers

    Which of the following pathogens is classified as a Category 4 organism?

    <p>Ebola virus (B)</p> Signup and view all the answers

    What is a key characteristic of BSL-3 facilities?

    <p>All work is done under negative air pressure. (C)</p> Signup and view all the answers

    Which classification method involves the genetic makeup of organisms?

    <p>Genotype (D)</p> Signup and view all the answers

    Which of the following features is NOT associated with ACDP Category 4 organisms?

    <p>Effective treatment options are readily available. (B)</p> Signup and view all the answers

    What type of mutation involves a single nucleotide change in DNA?

    <p>Point mutation (D)</p> Signup and view all the answers

    Which of the following best describes phylogenetics?

    <p>The study of evolutionary relationships among biological entities. (D)</p> Signup and view all the answers

    What does MSA stand for in the context of DNA sequence analysis?

    <p>Multiple Sequence Alignment (A)</p> Signup and view all the answers

    Which of the following statements is true regarding phylogenetic trees?

    <p>Rooted trees illustrate both how organisms are related and their ancestral lineage. (C)</p> Signup and view all the answers

    What does a high percentage of similarity between two bacterial strains in a genetic distance analysis indicate?

    <p>They are closely related with a similar genetic makeup. (A)</p> Signup and view all the answers

    Which of the following methods is least likely to be used for constructing phylogenetic trees?

    <p>Gene insertion and deletion tracking (D)</p> Signup and view all the answers

    What is a potential outcome of gene mutations in bacteria?

    <p>Enhanced resistance to antibiotics. (B)</p> Signup and view all the answers

    How are clades within phylogenetics defined?

    <p>By clusters of organisms sharing similar DNA or protein sequences. (A)</p> Signup and view all the answers

    What is the primary focus of biological systematics?

    <p>Assessing how life changes over time (D)</p> Signup and view all the answers

    Which of the following correctly distinguishes cellular microorganisms from acellular ones?

    <p>Cellular microorganisms can reproduce independently while acellular cannot (C)</p> Signup and view all the answers

    Which classification system is NOT traditionally used for acellular entities?

    <p>Linnaean system (C)</p> Signup and view all the answers

    In which of the following groups do viruses belong, according to the classification of biological entities?

    <p>Acellular organisms (B)</p> Signup and view all the answers

    Who contributed to the classification system by proposing 5 kingdoms?

    <p>Whittaker (D)</p> Signup and view all the answers

    What is a defining characteristic of prions?

    <p>They are misfolded proteins that do not reproduce (D)</p> Signup and view all the answers

    Which of the following ranks comes directly before the genus in taxonomic classification?

    <p>Family (C)</p> Signup and view all the answers

    What is the structural basis for the classification of viruses?

    <p>Their nucleic acid type and phenotype (C)</p> Signup and view all the answers

    Which domain includes organisms that possess a nuclear membrane?

    <p>Eukaryota (B)</p> Signup and view all the answers

    What did Woese contribute to the classification of biological entities?

    <p>The introduction of the three-domain classification system (A)</p> Signup and view all the answers

    Which of the following best defines a species in the context of living organisms?

    <p>A group of organisms capable of interbreeding, even if geographically isolated. (A)</p> Signup and view all the answers

    What is meant by a 'strain' in microbiology?

    <p>A genetic variant of a bacterial species differing slightly from its members. (A)</p> Signup and view all the answers

    Which classification provides the least detailed information about an organism?

    <p>Phenotype classification (A)</p> Signup and view all the answers

    In microbiology, serotypes are defined by what characteristic?

    <p>Distinct variations in cell surface antigens within a species. (C)</p> Signup and view all the answers

    Which statement about vertical and horizontal gene transfer is true?

    <p>Horizontal transfer allows for the acquisition of genetic information from non-parental organisms. (B)</p> Signup and view all the answers

    Which method is NOT typically associated with genotype classification?

    <p>Phenotype observation (C)</p> Signup and view all the answers

    What is the primary purpose of whole genome sequencing in microbiology?

    <p>To determine the complete DNA sequence of an organism at a single time. (B)</p> Signup and view all the answers

    What is the significance of 95% genome sequence similarity in bacterial species classification?

    <p>It is a threshold for defining bacterial species. (B)</p> Signup and view all the answers

    Which of the following biological traits is NOT considered in phenotype classification?

    <p>Genetic makeup of the organism (C)</p> Signup and view all the answers

    Which specific antigens are utilized to classify serotypes within the Salmonella genus?

    <p>Surface oligosaccharides and flagellar proteins (C)</p> Signup and view all the answers

    Flashcards

    Taxonomy

    The study of how organisms are classified based on shared characteristics.

    Classification

    Arranging organisms into groups based on their shared features, it's like sorting laundry by color.

    Nomenclature

    The process of assigning specific names to organisms. It's like giving everyone in your family a unique nickname.

    Cellular Organisms

    Living beings that are composed of cells, including those with a nucleus (Eukarya) and those without (Bacteria and Archaea).

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    Acellular Organisms

    Living beings that lack cells, such as viruses, viroids, and prions. They require a host to reproduce.

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    Taxonomic Ranks

    A hierarchical system used to classify living organisms, spanning from broad categories like 'Domain' down to specific species. It's like a family tree for all life.

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    Domain

    The broadest category in the taxonomic hierarchy, encompassing all living organisms. Think of it as the grandparent category.

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    Kingdom

    A group of organisms with a shared set of characteristics. It's like a big family with similar traits.

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    Phylum

    A group of organisms within a Kingdom, often with distinctive physical features. It's like a branch of the family tree.

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    Class

    A group of organisms within a Phylum, with shared anatomical features. It's like a specific group within a family, like cousins.

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    What is the ACDP?

    A system for classifying the risk posed by microorganisms to humans and the environment, with four categories ranging from minimal to severe.

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    What are Category 2 organisms?

    A group of microorganisms that pose a potential threat to human health, requiring specific laboratory facilities and safety protocols.

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    What is Biosafety?

    A set of precautions and levels of containment used in laboratories to handle dangerous microorganisms safely.

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    What is a BSL-2 facility?

    A laboratory environment designed to prevent the accidental release of dangerous microorganisms, with specific safety regulations and equipment.

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    What is a Biosafety Cabinet?

    A type of safety cabinet used in laboratories to protect workers and the environment from potentially infectious agents.

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    Point mutation (SNP)

    A specific change in a single DNA base, potentially altering the protein encoded by a gene.

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    Phylogenetics

    The study of evolutionary relationships between organisms, often visualized as branching diagrams.

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    Multiple Sequence Alignment (MSA)

    Aligned sequences of DNA or protein from different organisms, used to compare their similarities and differences.

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    Genetic distance (% similarity)

    A measure of how similar two genetic sequences are, expressed as a percentage.

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    Phylogenetic tree

    A diagram that represents the evolutionary relatedness between organisms, showing the branching pattern of their ancestry.

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    Rooted phylogenetic tree

    A phylogenetic tree where a common ancestor is identified, providing a timeline of evolutionary history.

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    Unrooted phylogenetic tree

    A phylogenetic tree that doesn't show a common ancestor, but only the relationship between organisms.

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    Horizontal gene transfer

    The process of acquiring new genes from other organisms, often through transfer of DNA.

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    What is a species?

    A group of living organisms capable of interbreeding, even if geographically isolated.

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    What is a species in microbiology?

    A collection of strains sharing stable properties, but significantly different from other groups.

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    What is a bacterial strain?

    A genetic variant or subtype of a bacterial species, varying slightly from others in the same species.

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    What is a phenotype?

    Observable characteristics or traits of an organism, including morphology, development, biochemistry or physiological properties.

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    What is a serotype?

    Distinct variations in cell surface antigens within a species.

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    What is a genotype?

    The part of the genetic makeup of a cell that determines one of its characteristics.

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    What is whole genome sequencing?

    The complete DNA sequence of an organism at a single time.

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    What is vertical transmission?

    The passage of genetic information from parent to offspring.

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    What is horizontal transmission?

    The acquisition of genetic information by transfer from an organism that is not its parent.

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    How are bacterial species defined using genomics?

    Genomes of bacterial species are at least 95% identical. However, horizontal gene transfer can complicate this definition.

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    BSL-3 Facility

    A laboratory designed to handle agents that pose a serious risk to human health, requiring stringent safety protocols and specialized equipment.

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    Phenotype

    A type of classification based on the observable characteristics of a microorganism, like its shape or its ability to grow in specific environments.

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    Category 4 Organisms

    Microbial agents that cause severe human disease often resulting in death, with a high risk of spreading within a population and lacking effective treatment options.

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    Biosafety Level

    A system for classifying microorganisms based on their potential risk to human health, animals, and the environment. Each level indicates increasing containment measures needed for safety.

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    Genotype

    A type of classification based on the genetic makeup of a microorganism, often using techniques like DNA sequencing.

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    Study Notes

    Microbial Classification

    • The MPharm programme includes a module on microbial classification
    • Learning outcomes include describing differences between cellular and acellular microorganisms, the main taxonomic groups and their features, and microbial classification with examples
    • Biological systematics studies how life changes over time and relates organisms to one another
    • Taxonomy defines systems using shared characteristics
    • Classification arranges organisms into groups
    • Nomenclature assigns names
    • Living things can be divided into cellular and acellular entities

    Classification of Biological Entities

    • Biological entities are either cellular or acellular
    • Cellular entities are further subdivided into Eukarya, Archaea, and Bacteria
    • Acellular entities include viruses, viroids, virusoids, and prions

    The Tree of Life

    • The tree of life illustrates evolutionary relationships among organisms
    • The tree displays a branching structure reflecting evolutionary lineages
    • Bacteria and Archaea represent distinct domains of life, separate from the Eukarya domain

    Development of Classification Systems

    • Various scientists have contributed to the development of classification systems
    • Historical examples include Linnaeus, Haeckel, Chatton, Whittaker, and Woese
    • Their contributions led to the evolution of understanding living systems overtime

    Taxonomic Ranks

    • Domain, Phylum, Class, Order, Family, Subfamily, Genus, and Species are used to formally identify and classify life
    • The example provided classifies Homo sapiens (humans) and Escherichia coli bacteria
    • Examples of species are the tiger and lion, which can interbreed
    • A species is a group of organisms that can interbreed. This also applies to organisms that do not reproduce sexually
    • Strains are genetic variants within a species.
    • The characteristics, methods, and examples for classifying organisms using phenotypes, serotypes, and genotypes are mentioned.

    Phenotype Classification

    • Morphology: Cell shape (bacilli or cocci), cell structure (gram staining). Examples given include bacteria.
    • Biochemistry: Enzyme production, transport proteins. Examples given include testing various enzymes
    • Life cycle: Vegetative or spore formation. Examples provided may vary.
    • Ecological niche: Temperature (thermophile, psychrophile).
    • Interactions with other organisms: Susceptibility to bacteriophages.
    • Pathogenicity: Ability to cause disease

    Serotype Classification

    • Serotype: Distinct variations of cell surface antigens within a species. Examples given include Salmonella and Vibrio cholerae
    • O and H antigens are used for identification.

    Genotype Classification

    • Genotype: The part of the genetic makeup of a cell determining characteristics (phenotype)
    • Genomics, PCR, base composition, nucleic acid sequencing, 16S rRNA, whole genome sequencing. Examples shown may vary.
    • Some species can be classified upon their DNA, or protein sequences, or genomic makeup
    • Whole genome sequencing provides complete DNA sequencing at one time
    • Bacterial species can be defined when genomes share at least 95% identical sequences.

    Vertical and Horizontal Gene Transfer

    • Vertical transmission: Genetic information passed from parent to offspring
    • Horizontal transmission: Genetic material acquired from a different organism, not a biological parent.

    Whole Genome Sequencing

    • Determines the full DNA sequence
    • Shows genes present or absent
    • Helps identify mutations, insertions, and deletions, and potentially identify horizontal gene transfer events.

    Visualising Genotypes: Phylogenetics

    • Phylogenetics studies evolutionary relations amongst organisms
    • Phylogenetic trees can be rooted or unrooted
    • Rooted trees infer the most recent common ancestor, whereas unrooted trees only show evolutionary relationships without inferring a common ancestor.
    • Multiple sequence alignments (MSAs) can be generated on DNA or protein sequences of biological entities, and these trees can be based on the genetic distance of the MSA.

    Practical Application of Classification: Biosafety

    • Microorganisms are categorized by the Advisory committee on Dangerous Pathogens (ACDP) into 4 levels.
    • Organisms categorized by the level of hazard that they pose to humans or the environment.
    • Biosafety Levels (BSLs) determine the appropriate safety precautions and laboratory facilities.
    • BSL-1 facilities require only general protective equipment, hand washing, and sterilization of materials.
    • BSL-2 requires all of BSL-1 precautions plus highly regulated design, procedures, trained personnel, restricted access to laboratory, and work can be performed in BSL cabinets.
    • BSL-3 facilities require enhanced conditions for BSL-2 and additional approvals for some pathogens
    • BSL-4 requires highly trained personnel, specific PPE, negative air pressure, and other strict safety measures for especially dangerous pathogens.

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    Description

    This quiz tests your knowledge on the classification of microorganisms according to ACDP categories and BSL levels. Questions cover key characteristics, facility classifications, and mutation types related to various organisms. Perfect for microbiology students and professionals.

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