Podcast
Questions and Answers
A dose of ______ grays is considered lethal to human cells.
A dose of ______ grays is considered lethal to human cells.
10
Bacteriophage therapy uses ______ specific viruses to target bacteria.
Bacteriophage therapy uses ______ specific viruses to target bacteria.
bacteria
Maggots therapy involves the use of ______ chemical factories that produce complex mixtures of biologically active molecules.
Maggots therapy involves the use of ______ chemical factories that produce complex mixtures of biologically active molecules.
living
Clostridium perfringens has a radiation resistance of ______ units.
Clostridium perfringens has a radiation resistance of ______ units.
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The Coxsackievirus has a radiation resistance of ______ units.
The Coxsackievirus has a radiation resistance of ______ units.
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Bacteriophage therapy relies on the host ______ and replication machinery to produce new phage particles.
Bacteriophage therapy relies on the host ______ and replication machinery to produce new phage particles.
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Maggots therapy prevents localized ______ and infection through the production of proteolytic enzymes and antibacterial molecules.
Maggots therapy prevents localized ______ and infection through the production of proteolytic enzymes and antibacterial molecules.
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Deinococcus radiodurans has a radiation resistance of ______ units.
Deinococcus radiodurans has a radiation resistance of ______ units.
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FMDV has a radiation resistance of ______ units.
FMDV has a radiation resistance of ______ units.
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The unit of absorbed radiation is measured in ______.
The unit of absorbed radiation is measured in ______.
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Study Notes
Killing Kinetics
- D value (Decimal reduction time) is the time required to reduce the viable population by a factor of 10 (time to kill 90%) under defined conditions.
- D value is used to quantify the probability of an item/area remaining unsterile despite undergoing sterilization process.
Sterilization Methods
- Heat causes loss of structure and function to the fundamental macromolecules of bacterial cells (proteins, DNA, cell membrane).
- Thermal death time is the time it takes to kill all cells at a given temperature.
- Condensation onto cooler surfaces releases latent heat.
- Dry heat vs Moist heat: Most effective after prior evacuation (autoclave).
Media and Growth
- Mannitol Salt Agar is a selective and differential media that differentiates Staphylococcus aureus and Staphylococcus epidermidis based on mannitol fermentation and salt tolerance.
- Blood Agar is an enrichment media (Sheep blood) that may also act as a differential agar, differentiating bacteria based on haemolytic activity.
- Chocolate Agar is a variation of blood agar, heated to >80℃ to lyse red blood cells, providing growth factors and co-enzymes for fastidious organisms like Haemophilus influenzae and Neisseria meningitidis.
Biological Control
- Bacteriophage Therapy uses bacteria-specific viruses that replicate within host cells to produce new phage particles.
- Maggots Therapy uses living chemical factories that produce biologically active molecules, including proteolytic enzymes and antibacterial molecules, to prevent localized colonization and infection.
Other Key Facts
- Presence of bile salts inhibits Gram-positive growth.
- High [salt] inhibits Gram-negative growth.
- Human cells can tolerate a lethal dose of 10 Grays of radiation.
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Description
This quiz is about the viable count of different organisms under various treatments, including treatment A, treatment B, and treatment C. It involves understanding the concept of viable count and its relation to time.
